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The map label for this gene is grpE [H]

Identifier: 154246474

GI number: 154246474

Start: 2829943

End: 2830596

Strand: Reverse

Name: grpE [H]

Synonym: Xaut_2533

Alternate gene names: 154246474

Gene position: 2830596-2829943 (Counterclockwise)

Preceding gene: 154246475

Following gene: 154246471

Centisome position: 53.32

GC content: 69.72

Gene sequence:

>654_bases
ATGAGCGAGCAGAAGCGCGCGCCCGAGACCGGCGGCGACGCAAGCGAAAACGGTGTGAACGCGGGCGTCAACGAGGCGAC
CGCCGAGGCCGAAGCCCTCGCGACCGCCGACCAGGAGATCGGGGGCGACTTCGCTGCGGAAAAGCAGCGCCTGGAAGCCG
AGATCGCGAGCCTGAAGGACAAGTTCCTGCGCGCCTTCGCCGAGGCGGAGAACATCCGTCGCCGCGCCGAGAAGGAAGTG
GTGGACGCCAAGACCTACGGCATCGCCTCCTTCGCCCGCGACGTGCTGAACGTGGCCGACGACCTCGCCCGCGCCCTCGG
CACGGTGGACGAGGAGGCCAAGGCCACCGCCGACGGCGCCGTGAAGGGCCTTTTGGAAGGGCTGGAGCTGACCGAGCGCG
GCCTCGTCAAGGCGCTGGAAAAGCACGGCATCCGCAAGATCGAGCCCAAGGGCGAGAAGTTCGACCCCAACCTGCATCAG
GCCATGTTCGAGGTGCCCGACCCGAGCGTGCCGTCGGGCACGGTGGTGCAGGTGGTGCAGTCCGGCTACGTCATCGGCGA
GCGGGTGCTGCGGCCGGCCATGGTGGGTGTCGCCCGCGGTGGGCCGAAGGCCGAGGCCACATCCGGCAAGGACAAGGAAG
CCGGGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCTCGCGCAGTCTTGATTTTTCCCGCCGTGGCCCCGATATCCGCGTTGACATTAGGCATTTGCCCCTGCGGGGGCGACAT
TCATGGCAGGGGATAACCGG

Downstream 100 bases:

>100_bases
CCCTCTGGGCCTGTCGCCGTTATTGTCCCCTACACGAAGGGCCGGAGCACCTTGATCGACGGCCTTTGGTCCAGCCCGAT
TGGCGAAGCCTTAGTCGGAA

Product: ribulose-phosphate 3-epimerase

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSEQKRAPETGGDASENGVNAGVNEATAEAEALATADQEIGGDFAAEKQRLEAEIASLKDKFLRAFAEAENIRRRAEKEV
VDAKTYGIASFARDVLNVADDLARALGTVDEEAKATADGAVKGLLEGLELTERGLVKALEKHGIRKIEPKGEKFDPNLHQ
AMFEVPDPSVPSGTVVQVVQSGYVIGERVLRPAMVGVARGGPKAEATSGKDKEAGAA

Sequences:

>Translated_217_residues
MSEQKRAPETGGDASENGVNAGVNEATAEAEALATADQEIGGDFAAEKQRLEAEIASLKDKFLRAFAEAENIRRRAEKEV
VDAKTYGIASFARDVLNVADDLARALGTVDEEAKATADGAVKGLLEGLELTERGLVKALEKHGIRKIEPKGEKFDPNLHQ
AMFEVPDPSVPSGTVVQVVQSGYVIGERVLRPAMVGVARGGPKAEATSGKDKEAGAA
>Mature_216_residues
SEQKRAPETGGDASENGVNAGVNEATAEAEALATADQEIGGDFAAEKQRLEAEIASLKDKFLRAFAEAENIRRRAEKEVV
DAKTYGIASFARDVLNVADDLARALGTVDEEAKATADGAVKGLLEGLELTERGLVKALEKHGIRKIEPKGEKFDPNLHQA
MFEVPDPSVPSGTVVQVVQSGYVIGERVLRPAMVGVARGGPKAEATSGKDKEAGAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=153, Percent_Identity=42.483660130719, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI40255109, Length=155, Percent_Identity=40, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788967, Length=154, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=42, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324806, Length=162, Percent_Identity=35.8024691358025, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653432, Length=145, Percent_Identity=42.0689655172414, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22854; Mature: 22723

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKRAPETGGDASENGVNAGVNEATAEAEALATADQEIGGDFAAEKQRLEAEIASLKD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KFLRAFAEAENIRRRAEKEVVDAKTYGIASFARDVLNVADDLARALGTVDEEAKATADGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VKGLLEGLELTERGLVKALEKHGIRKIEPKGEKFDPNLHQAMFEVPDPSVPSGTVVQVVQ
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
SGYVIGERVLRPAMVGVARGGPKAEATSGKDKEAGAA
HCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEQKRAPETGGDASENGVNAGVNEATAEAEALATADQEIGGDFAAEKQRLEAEIASLKD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KFLRAFAEAENIRRRAEKEVVDAKTYGIASFARDVLNVADDLARALGTVDEEAKATADGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VKGLLEGLELTERGLVKALEKHGIRKIEPKGEKFDPNLHQAMFEVPDPSVPSGTVVQVVQ
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
SGYVIGERVLRPAMVGVARGGPKAEATSGKDKEAGAA
HCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA