Click here to switch to the map view.

The map label for this gene is nirA

Identifier: 154246440

GI number: 154246440

Start: 2792984

End: 2794744

Strand: Reverse

Name: nirA

Synonym: Xaut_2499

Alternate gene names: 154246440

Gene position: 2794744-2792984 (Counterclockwise)

Preceding gene: 154246441

Following gene: 154246439

Centisome position: 52.64

GC content: 69.28

Gene sequence:

>1761_bases
ATGGGCAGCGACTTTACCATCGACCAGAAACGCTATCTGGAAGGCTTCGTCTCCGGCGCCAAGGCGGTGCAGATCACCCG
GGGCGCTGCGCCCGCAGGCGCGCCTGCCGCTGCCGCCGAGCCCACCGGCCCCGACGCAGCGAGCCTGAAGGCCATGGCTC
GCTTCGAGGCGGAGGGCAAAAAGCTCTCGCCGGAGGAGAAGGGCAAGCGCGAGGAACTGGGCCTTGATGCCTACCCCCGC
ATGAGGACCGCCGCCCACGAGGGCGTCTTTCCCAAGGGCCCGGACGTGCTCCGCTGGAAGTACCACGGTCTGTTCCATGT
GGCGCCGGCGCAGGACAGCTTCATGTGCCGCCTGCGCATTCCCAATGGCATCCTCACCCACTGGCAGTTTCGCGGCGTCG
CCGACATCGCGTCCGCCCACGGCGGCAACTACACCGACGTGACCACGCGGGCCAATCTGCAGATCCGCGAGATCCCGGCC
ACCGACGGCATCGCCGTGCTGGAAAAACTGGTGGACCTCGGCCTCACCGCCAAGGGCTCTGGGGCGGACAATATCCGCAA
CGTGACCGGCACCCCCACCGCCGGCATCGACCCGCAGGAGTTGCTCGACACCCGGCCGCTGGCGAAGGAGTGGCACCACT
ACATCCTCAACAATCGCGACATGTACGGCCTGCCGCGCAAGTTCAACGTGGCCTTCGACGGCGCCGGCACCATCGGCGCG
CTGGAGGACACCAATGACATCGGCTTCCAGGCGGTGGAGGTGAAGGACGGGTTCGGCGTGGCGCCGGGCATCTGGCTGCG
GCTCGCCCTGGGCGGCATCACCGGCCACAAGGATTTCGCCCGCGACACCGGCATCGTGGTGCGCCCCGACGAGGCGAGCG
ACGTGGCCGCCGCCATCGTGCGCGCCTTCATCGACACCGGCGACCGCACCGACCGCAAGAAGGCGCGGCTGAAATACGTG
CTCGACGCCATGGGCTTCGACGGTTTCCTGACGCTGGTGGAAAAGAAGCTGGGCCGTCCCTTCACCCGCCTGCCGGCGGA
AGCGGTGGCACCCCGCGCCGAGGCCGACCGCCTCGCCCATCTCGGCGTTCACGCCCAGAAGCAGCCGGGGCTGAATTACA
TCGGCGTCGGCCTGCCGGTGGGCCGCATGAGCGTGGAGCAGATGCACGGCCTCGCTGACATCGCCGCGCGCCTCGGCGAC
GGCGACATCCGCCTCACGGTCTGGCAGAACCTGCTCATCTCGGGCGTTGCCGACGCGGACGTGGCGCAGGCGTGCACCGC
CATCACGGCGCTCGGGCTGTCCACCCAGGCGACGCCGCTCCAGGCCGGCCTTGTGGCCTGCACGGGATCCAAGGGCTGCC
GCTTCGCCGCCGCCGACACCAAGGGCACGGCCCGCGCCATCCTCGCCCATTGCGAGGAACGCCTCGACCTCGACGGGCCG
GTGAACATCCATGTCACCGGCTGCCACAATTCCTGCGCCCAGCATTATATCGGCGACATCGGCCTCATCGGCGCGCGGGT
GGCCATCAATGACGATGGCGACACGGTGGACGGCTATCACCTGTTCGTCGGCGGCGGATTCGGCGCGGACGCCGCCATCG
CCCGCGAACTCTATCGCGACGTGAAGGCGGAAGACGCCCCCGCGGTGGTGGAGCGCCTGCTGAAGAGCTGGCTCGCCCAC
CGCGAGGAGGGCGAGGCATTCGCGCACTTCACCCGCCGCATGGACCCCGACGCGCTGAAGGCGCTGGCGGAGGTGGCATG
A

Upstream 100 bases:

>100_bases
ATCTCGACCTCATCACCCAACGCACCCCCATCACCGCCATGCGCGCCGATCTCGCCTTCGGGCGGGACGCCATCGCCGCG
GCGGCATGAGGAGAGACCCC

Downstream 100 bases:

>100_bases
CGCCCAACCAGCCGGGCCGCACTGCGGCCTGACGCTCGATTTCGCGCCCAAGGCGCGCCACCCGACACCGAGACCACAGC
CATGAGCCTCCAGACCCGCA

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MGSDFTIDQKRYLEGFVSGAKAVQITRGAAPAGAPAAAAEPTGPDAASLKAMARFEAEGKKLSPEEKGKREELGLDAYPR
MRTAAHEGVFPKGPDVLRWKYHGLFHVAPAQDSFMCRLRIPNGILTHWQFRGVADIASAHGGNYTDVTTRANLQIREIPA
TDGIAVLEKLVDLGLTAKGSGADNIRNVTGTPTAGIDPQELLDTRPLAKEWHHYILNNRDMYGLPRKFNVAFDGAGTIGA
LEDTNDIGFQAVEVKDGFGVAPGIWLRLALGGITGHKDFARDTGIVVRPDEASDVAAAIVRAFIDTGDRTDRKKARLKYV
LDAMGFDGFLTLVEKKLGRPFTRLPAEAVAPRAEADRLAHLGVHAQKQPGLNYIGVGLPVGRMSVEQMHGLADIAARLGD
GDIRLTVWQNLLISGVADADVAQACTAITALGLSTQATPLQAGLVACTGSKGCRFAAADTKGTARAILAHCEERLDLDGP
VNIHVTGCHNSCAQHYIGDIGLIGARVAINDDGDTVDGYHLFVGGGFGADAAIARELYRDVKAEDAPAVVERLLKSWLAH
REEGEAFAHFTRRMDPDALKALAEVA

Sequences:

>Translated_586_residues
MGSDFTIDQKRYLEGFVSGAKAVQITRGAAPAGAPAAAAEPTGPDAASLKAMARFEAEGKKLSPEEKGKREELGLDAYPR
MRTAAHEGVFPKGPDVLRWKYHGLFHVAPAQDSFMCRLRIPNGILTHWQFRGVADIASAHGGNYTDVTTRANLQIREIPA
TDGIAVLEKLVDLGLTAKGSGADNIRNVTGTPTAGIDPQELLDTRPLAKEWHHYILNNRDMYGLPRKFNVAFDGAGTIGA
LEDTNDIGFQAVEVKDGFGVAPGIWLRLALGGITGHKDFARDTGIVVRPDEASDVAAAIVRAFIDTGDRTDRKKARLKYV
LDAMGFDGFLTLVEKKLGRPFTRLPAEAVAPRAEADRLAHLGVHAQKQPGLNYIGVGLPVGRMSVEQMHGLADIAARLGD
GDIRLTVWQNLLISGVADADVAQACTAITALGLSTQATPLQAGLVACTGSKGCRFAAADTKGTARAILAHCEERLDLDGP
VNIHVTGCHNSCAQHYIGDIGLIGARVAINDDGDTVDGYHLFVGGGFGADAAIARELYRDVKAEDAPAVVERLLKSWLAH
REEGEAFAHFTRRMDPDALKALAEVA
>Mature_585_residues
GSDFTIDQKRYLEGFVSGAKAVQITRGAAPAGAPAAAAEPTGPDAASLKAMARFEAEGKKLSPEEKGKREELGLDAYPRM
RTAAHEGVFPKGPDVLRWKYHGLFHVAPAQDSFMCRLRIPNGILTHWQFRGVADIASAHGGNYTDVTTRANLQIREIPAT
DGIAVLEKLVDLGLTAKGSGADNIRNVTGTPTAGIDPQELLDTRPLAKEWHHYILNNRDMYGLPRKFNVAFDGAGTIGAL
EDTNDIGFQAVEVKDGFGVAPGIWLRLALGGITGHKDFARDTGIVVRPDEASDVAAAIVRAFIDTGDRTDRKKARLKYVL
DAMGFDGFLTLVEKKLGRPFTRLPAEAVAPRAEADRLAHLGVHAQKQPGLNYIGVGLPVGRMSVEQMHGLADIAARLGDG
DIRLTVWQNLLISGVADADVAQACTAITALGLSTQATPLQAGLVACTGSKGCRFAAADTKGTARAILAHCEERLDLDGPV
NIHVTGCHNSCAQHYIGDIGLIGARVAINDDGDTVDGYHLFVGGGFGADAAIARELYRDVKAEDAPAVVERLLKSWLAHR
EEGEAFAHFTRRMDPDALKALAEVA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=537, Percent_Identity=24.7672253258845, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322597, Length=536, Percent_Identity=25.1865671641791, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 62535; Mature: 62403

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDFTIDQKRYLEGFVSGAKAVQITRGAAPAGAPAAAAEPTGPDAASLKAMARFEAEGK
CCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
KLSPEEKGKREELGLDAYPRMRTAAHEGVFPKGPDVLRWKYHGLFHVAPAQDSFMCRLRI
CCCCHHCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCEEEEEEC
PNGILTHWQFRGVADIASAHGGNYTDVTTRANLQIREIPATDGIAVLEKLVDLGLTAKGS
CCCCCCCEECCCHHHHHHCCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCC
GADNIRNVTGTPTAGIDPQELLDTRPLAKEWHHYILNNRDMYGLPRKFNVAFDGAGTIGA
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEC
LEDTNDIGFQAVEVKDGFGVAPGIWLRLALGGITGHKDFARDTGIVVRPDEASDVAAAIV
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHHHH
RAFIDTGDRTDRKKARLKYVLDAMGFDGFLTLVEKKLGRPFTRLPAEAVAPRAEADRLAH
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHH
LGVHAQKQPGLNYIGVGLPVGRMSVEQMHGLADIAARLGDGDIRLTVWQNLLISGVADAD
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHH
VAQACTAITALGLSTQATPLQAGLVACTGSKGCRFAAADTKGTARAILAHCEERLDLDGP
HHHHHHHHHHHCCCCCCCCHHCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
VNIHVTGCHNSCAQHYIGDIGLIGARVAINDDGDTVDGYHLFVGGGFGADAAIARELYRD
EEEEEECCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
VKAEDAPAVVERLLKSWLAHREEGEAFAHFTRRMDPDALKALAEVA
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
GSDFTIDQKRYLEGFVSGAKAVQITRGAAPAGAPAAAAEPTGPDAASLKAMARFEAEGK
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
KLSPEEKGKREELGLDAYPRMRTAAHEGVFPKGPDVLRWKYHGLFHVAPAQDSFMCRLRI
CCCCHHCCCHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCEEEEEEC
PNGILTHWQFRGVADIASAHGGNYTDVTTRANLQIREIPATDGIAVLEKLVDLGLTAKGS
CCCCCCCEECCCHHHHHHCCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCC
GADNIRNVTGTPTAGIDPQELLDTRPLAKEWHHYILNNRDMYGLPRKFNVAFDGAGTIGA
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEC
LEDTNDIGFQAVEVKDGFGVAPGIWLRLALGGITGHKDFARDTGIVVRPDEASDVAAAIV
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHHHH
RAFIDTGDRTDRKKARLKYVLDAMGFDGFLTLVEKKLGRPFTRLPAEAVAPRAEADRLAH
HHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHH
LGVHAQKQPGLNYIGVGLPVGRMSVEQMHGLADIAARLGDGDIRLTVWQNLLISGVADAD
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHH
VAQACTAITALGLSTQATPLQAGLVACTGSKGCRFAAADTKGTARAILAHCEERLDLDGP
HHHHHHHHHHHCCCCCCCCHHCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCC
VNIHVTGCHNSCAQHYIGDIGLIGARVAINDDGDTVDGYHLFVGGGFGADAAIARELYRD
EEEEEECCCHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
VKAEDAPAVVERLLKSWLAHREEGEAFAHFTRRMDPDALKALAEVA
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]