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The map label for this gene is nasD [H]

Identifier: 154246441

GI number: 154246441

Start: 2794756

End: 2795976

Strand: Reverse

Name: nasD [H]

Synonym: Xaut_2500

Alternate gene names: 154246441

Gene position: 2795976-2794756 (Counterclockwise)

Preceding gene: 154246442

Following gene: 154246440

Centisome position: 52.67

GC content: 70.68

Gene sequence:

>1221_bases
ATGAGCGAGCCCCTCCTCATCATCGGCAACGGCATGGCGGCGGCAAAGCTCTGCGAGGAGCTGAGCCAGCGCGCGCTCGG
CCGCTATGCGGTCGCGGTGATCGGGGCGGAGCCGCACCTCGCCTACAACCGGGTGCTGCTGTCGGAGGTGCTCGCCGGCC
ACATGACCGCCGCGGACGCGCAGATGAAGCCCGCCTCCTGGTGGCGCGATCGCGGCGTGACCCTGCAATACGGCACCCCC
GCCGTCGCCATCGACCGCACCGAGCGCGCCGTCGTGCTAGCGGATGGGCGGCGCCTGTCCTATGCGCGCCTCGTGCTCGC
CACCGGCTCGCATCCCATCCGCCTGCCGTTGCCGGGGATGGACCTGAACGGGGTCCACACCTTCCGCGACCTGAAGGATG
TGGAGGCGCTGCTCGCCGCCGCCGCCGTTCCCGGCACAAGGGCGGTGGTAATCGGCGGCGGGCTGCTGGGGCTTGAGGCC
GCCGTGGGCCTTGCCGGGGCGGGAGTGAAGACAAGCCTCGTCCATCTCATGGACCGGCTGATGGAGCGCCAGCTCGATCC
CGCCGCCGCCGGATTTCTGCGGCGGGCGGTGGAAGGCAAGGACGTCGAGGTGCTGCTTCAGGCCGCCAGCGACGCCATCG
AGGGGGATGAGCAGGGCCGCGTCACTGGCCTCAGACTCAAAGATGGGCGCGTGGTGGAGGCCGACCTCGTGGTGGTGGCC
TGCGGCGTCATCCCCAATGCGGACCTTGCCCGCGCGGCGGGGCTGGAGGTGAAGCGCGGCATCGTGGTGGACGACACCCT
TGCCACCTCCGATCCCGCCATCTGCGCCATCGGCGAATGCGCCGAGCATCGCGGCATCGCCTATGGCCTTGTGGCGCCGG
CCTATGAGCAGGCCCGCGTGCTCGCCCACCGCCTCGCGGGGGAGGAGGCGCGGTACGACGGCTCGGTGATGGCCACCAAT
CTGAAGGTTTCGGGCGTTCCCGTCTTCTCGGCGGGCGACGTCACCGGCGGCGAGGGCACGGAAGACATCGTGCTCACCGA
CCGGGGGCTGGGCGTTTATCGCCGGCTCATCGTGAAGGACGGGCTCCTCGTCGGCGCCGTGCTGTTCGGCGATACGGCGG
ATGGCCTGTTCTATCTCGACCTCATCACCCAACGCACCCCCATCACCGCCATGCGCGCCGATCTCGCCTTCGGGCGGGAC
GCCATCGCCGCGGCGGCATGA

Upstream 100 bases:

>100_bases
CCCGAGGTGAAGGCCGCCTGGGCCGACGCCTACGGCCTGCTCTCCTCCGTCATGATCGCCGCCGCCGAAGCCCCCGAGCC
GGTGGCCTGAGGATCGCGAC

Downstream 100 bases:

>100_bases
GGAGAGACCCCATGGGCAGCGACTTTACCATCGACCAGAAACGCTATCTGGAAGGCTTCGTCTCCGGCGCCAAGGCGGTG
CAGATCACCCGGGGCGCTGC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSEPLLIIGNGMAAAKLCEELSQRALGRYAVAVIGAEPHLAYNRVLLSEVLAGHMTAADAQMKPASWWRDRGVTLQYGTP
AVAIDRTERAVVLADGRRLSYARLVLATGSHPIRLPLPGMDLNGVHTFRDLKDVEALLAAAAVPGTRAVVIGGGLLGLEA
AVGLAGAGVKTSLVHLMDRLMERQLDPAAAGFLRRAVEGKDVEVLLQAASDAIEGDEQGRVTGLRLKDGRVVEADLVVVA
CGVIPNADLARAAGLEVKRGIVVDDTLATSDPAICAIGECAEHRGIAYGLVAPAYEQARVLAHRLAGEEARYDGSVMATN
LKVSGVPVFSAGDVTGGEGTEDIVLTDRGLGVYRRLIVKDGLLVGAVLFGDTADGLFYLDLITQRTPITAMRADLAFGRD
AIAAAA

Sequences:

>Translated_406_residues
MSEPLLIIGNGMAAAKLCEELSQRALGRYAVAVIGAEPHLAYNRVLLSEVLAGHMTAADAQMKPASWWRDRGVTLQYGTP
AVAIDRTERAVVLADGRRLSYARLVLATGSHPIRLPLPGMDLNGVHTFRDLKDVEALLAAAAVPGTRAVVIGGGLLGLEA
AVGLAGAGVKTSLVHLMDRLMERQLDPAAAGFLRRAVEGKDVEVLLQAASDAIEGDEQGRVTGLRLKDGRVVEADLVVVA
CGVIPNADLARAAGLEVKRGIVVDDTLATSDPAICAIGECAEHRGIAYGLVAPAYEQARVLAHRLAGEEARYDGSVMATN
LKVSGVPVFSAGDVTGGEGTEDIVLTDRGLGVYRRLIVKDGLLVGAVLFGDTADGLFYLDLITQRTPITAMRADLAFGRD
AIAAAA
>Mature_405_residues
SEPLLIIGNGMAAAKLCEELSQRALGRYAVAVIGAEPHLAYNRVLLSEVLAGHMTAADAQMKPASWWRDRGVTLQYGTPA
VAIDRTERAVVLADGRRLSYARLVLATGSHPIRLPLPGMDLNGVHTFRDLKDVEALLAAAAVPGTRAVVIGGGLLGLEAA
VGLAGAGVKTSLVHLMDRLMERQLDPAAAGFLRRAVEGKDVEVLLQAASDAIEGDEQGRVTGLRLKDGRVVEADLVVVAC
GVIPNADLARAAGLEVKRGIVVDDTLATSDPAICAIGECAEHRGIAYGLVAPAYEQARVLAHRLAGEEARYDGSVMATNL
KVSGVPVFSAGDVTGGEGTEDIVLTDRGLGVYRRLIVKDGLLVGAVLFGDTADGLFYLDLITQRTPITAMRADLAFGRDA
IAAAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=359, Percent_Identity=26.4623955431755, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI65787454, Length=359, Percent_Identity=26.4623955431755, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI226437568, Length=359, Percent_Identity=26.4623955431755, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4757732, Length=251, Percent_Identity=28.6852589641434, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI22202629, Length=251, Percent_Identity=28.6852589641434, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789765, Length=379, Percent_Identity=36.1477572559367, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789065, Length=336, Percent_Identity=29.1666666666667, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=31.8996415770609, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787352, Length=348, Percent_Identity=27.2988505747126, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=279, Percent_Identity=29.0322580645161, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=345, Percent_Identity=27.536231884058, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24639257, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281359715, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281359713, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24639250, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18543267, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24639252, Length=415, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585130, Length=333, Percent_Identity=20.7207207207207, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28573993, Length=209, Percent_Identity=30.622009569378, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581020, Length=209, Percent_Identity=30.622009569378, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 42478; Mature: 42346

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLLIIGNGMAAAKLCEELSQRALGRYAVAVIGAEPHLAYNRVLLSEVLAGHMTAADA
CCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
QMKPASWWRDRGVTLQYGTPAVAIDRTERAVVLADGRRLSYARLVLATGSHPIRLPLPGM
CCCCHHHHHCCCEEEEECCCEEEEECCCCEEEEECCCEEEEEEEEEECCCCEEEECCCCC
DLNGVHTFRDLKDVEALLAAAAVPGTRAVVIGGGLLGLEAAVGLAGAGVKTSLVHLMDRL
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHH
MERQLDPAAAGFLRRAVEGKDVEVLLQAASDAIEGDEQGRVTGLRLKDGRVVEADLVVVA
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEE
CGVIPNADLARAAGLEVKRGIVVDDTLATSDPAICAIGECAEHRGIAYGLVAPAYEQARV
ECCCCCCHHHHHCCCEECCCEEEECCCCCCCCCEEEECHHHHHCCEEEEECCCCHHHHHH
LAHRLAGEEARYDGSVMATNLKVSGVPVFSAGDVTGGEGTEDIVLTDRGLGVYRRLIVKD
HHHHHCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHC
GLLVGAVLFGDTADGLFYLDLITQRTPITAMRADLAFGRDAIAAAA
CCEEEEEEECCCCCCEEEEEEHHCCCCCHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
SEPLLIIGNGMAAAKLCEELSQRALGRYAVAVIGAEPHLAYNRVLLSEVLAGHMTAADA
CCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
QMKPASWWRDRGVTLQYGTPAVAIDRTERAVVLADGRRLSYARLVLATGSHPIRLPLPGM
CCCCHHHHHCCCEEEEECCCEEEEECCCCEEEEECCCEEEEEEEEEECCCCEEEECCCCC
DLNGVHTFRDLKDVEALLAAAAVPGTRAVVIGGGLLGLEAAVGLAGAGVKTSLVHLMDRL
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHH
MERQLDPAAAGFLRRAVEGKDVEVLLQAASDAIEGDEQGRVTGLRLKDGRVVEADLVVVA
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEE
CGVIPNADLARAAGLEVKRGIVVDDTLATSDPAICAIGECAEHRGIAYGLVAPAYEQARV
ECCCCCCHHHHHCCCEECCCEEEECCCCCCCCCEEEECHHHHHCCEEEEECCCCHHHHHH
LAHRLAGEEARYDGSVMATNLKVSGVPVFSAGDVTGGEGTEDIVLTDRGLGVYRRLIVKD
HHHHHCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHC
GLLVGAVLFGDTADGLFYLDLITQRTPITAMRADLAFGRDAIAAAA
CCEEEEEEECCCCCCEEEEEEHHCCCCCHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]