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The map label for this gene is pyk [H]

Identifier: 154246312

GI number: 154246312

Start: 2645858

End: 2647414

Strand: Direct

Name: pyk [H]

Synonym: Xaut_2370

Alternate gene names: 154246312

Gene position: 2645858-2647414 (Clockwise)

Preceding gene: 154246311

Following gene: 154246313

Centisome position: 49.84

GC content: 68.34

Gene sequence:

>1557_bases
ATGCCCACGAAGCGCCAGCAGCCCCTGAAGCGCCAGCGTGCCACCAAGATCGTCGCCACCGTCGGCCCCGCATCCAACAG
CGAGGAGAAGCTGAAGGCGCTGTTCCTCGCCGGCGTGGACGTGTTCCGGCTCAACTTCTCCCATGGCACGCAGGAAAACC
ATGGCGAGGTGCTGGCCCGCATCCGCCGGCTGGAAAAGGAGATGAACCGGCCCATCGGCGTCATCGCCGACATGCAGGGC
CCCAAGCTGCGCATCGGCCGCTTCAAGGACGGCGCCATTGCCATCAAGGCTGGCGACATCCTGCATTTCGATGCCGACGT
GGACCGGCCGGGCGACGAGACCCGCGTGCCGATCCCGCACCCCGATATCCTCGCCGCGCTGAACGTGGGCTCCACCGTGC
TGTGCGACGACGGCAAGGTGCGCCTGAAGGTGGTCAAGAAGGGCGAGGGCTTCCTGGAGGCCCAGGTGGTGGCAGGCACC
CGTCTGTCCAACAACAAGGGCTTCAACATCCCCGACGTGGTGCTGCCGCTGTCGCCGCTCACCGACAAGGATCGCCTGGA
CCTGGAGTTTGCCTGCGAGGCGGGGGTGGAGTGGATCGCCCTCTCCTTCGTGCAGCGGCCGGAGGACATCCACGAGGCCC
GCGCGCTCATCAAGGACCGCGCGGCGGTGATGCTGAAGATGGAGAAGCCGTCGGCGGTGGACTATCTCACCGAGCTGGTG
GAGCTGTCCGATTCCATCATGGTGGCGCGCGGTGACCTCGGCGTGGAAATCTCGCTGGCCGAGGTGCCGGCGCTGCAGAA
GCGCATGATCGCCGAGGCCCGCAAGTTCGGCCGTCCGGTCATCGTCGCCACCCAAATGCTGGAATCCATGATCACCGCCC
CGGTGCCCACCCGTGCCGAGGTGTCGGACGTGGCCACCGCCGTCTATGAGGGTGCCGACTGCGTGATGCTTTCCGCGGAG
ACGGCGGCCGGCCAGTTCCCGGTGGAGGCCGTCACCTTCATGGACGACATCATCCGCCACGTGGAGTGCGATCCCGGCTA
CCGACAGGCGCTGGACGCCACCCAGGCGGAATGGGGCAAGACCATCGGCGACGCGGTCACCAAGGCCACCTACCAGGCCG
CCATCGCGGTGGATGCCTCGGCGCTGGTGGCCTTCACCCTCTCGGGCACCACGGGCCTGCGGGCCGCGCGCGAGCGCCCG
GCCATCCCGATCCTCGGCCTCACCCCGAAGATGGAGACGGCGCGTCGTCTCGCGTTGTCCTATGGCGTGCACGCCGTGCA
CATGAAGGAGGACATCCACTCCTTCGGCGACATGGTGGACACGGCGGTGCGCACCGCCGTGGAGCACGGCCTGGGCAAGC
CCGGGGACCGCCTGGCCATCACCGCCGGCGTGCCCTTCGCCAAGCCCGGCACCACCAACATCATGCGCATCACCACCATC
GGCGCCGACAGCCCGCCCTCCCGCCACGACGCCGACACGCCCGCGGTGCGCTCGACCAAGGCTCATGAGAGCAAGGGGCA
GGAGAAGAAGGCGCACGGCAGCAAGGTGCACGCCTGA

Upstream 100 bases:

>100_bases
ATGCGTACATGAAGGGCGCGCATGATGTCCCTAGAGACGCACGACTCAACAAGACGCACAACCAACGCGTGATCAAACGC
GGGAGCCGGAGGAAGACCCC

Downstream 100 bases:

>100_bases
GGCGATGCGGTGCGGCCGGGCAACTCCCGGCCGCACCAGCCGTTCTCGCATTCCACGATCCTGCCGCCGCCTTCTGCCGC
AGGCGGGCGCACGAAATCGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPTKRQQPLKRQRATKIVATVGPASNSEEKLKALFLAGVDVFRLNFSHGTQENHGEVLARIRRLEKEMNRPIGVIADMQG
PKLRIGRFKDGAIAIKAGDILHFDADVDRPGDETRVPIPHPDILAALNVGSTVLCDDGKVRLKVVKKGEGFLEAQVVAGT
RLSNNKGFNIPDVVLPLSPLTDKDRLDLEFACEAGVEWIALSFVQRPEDIHEARALIKDRAAVMLKMEKPSAVDYLTELV
ELSDSIMVARGDLGVEISLAEVPALQKRMIAEARKFGRPVIVATQMLESMITAPVPTRAEVSDVATAVYEGADCVMLSAE
TAAGQFPVEAVTFMDDIIRHVECDPGYRQALDATQAEWGKTIGDAVTKATYQAAIAVDASALVAFTLSGTTGLRAARERP
AIPILGLTPKMETARRLALSYGVHAVHMKEDIHSFGDMVDTAVRTAVEHGLGKPGDRLAITAGVPFAKPGTTNIMRITTI
GADSPPSRHDADTPAVRSTKAHESKGQEKKAHGSKVHA

Sequences:

>Translated_518_residues
MPTKRQQPLKRQRATKIVATVGPASNSEEKLKALFLAGVDVFRLNFSHGTQENHGEVLARIRRLEKEMNRPIGVIADMQG
PKLRIGRFKDGAIAIKAGDILHFDADVDRPGDETRVPIPHPDILAALNVGSTVLCDDGKVRLKVVKKGEGFLEAQVVAGT
RLSNNKGFNIPDVVLPLSPLTDKDRLDLEFACEAGVEWIALSFVQRPEDIHEARALIKDRAAVMLKMEKPSAVDYLTELV
ELSDSIMVARGDLGVEISLAEVPALQKRMIAEARKFGRPVIVATQMLESMITAPVPTRAEVSDVATAVYEGADCVMLSAE
TAAGQFPVEAVTFMDDIIRHVECDPGYRQALDATQAEWGKTIGDAVTKATYQAAIAVDASALVAFTLSGTTGLRAARERP
AIPILGLTPKMETARRLALSYGVHAVHMKEDIHSFGDMVDTAVRTAVEHGLGKPGDRLAITAGVPFAKPGTTNIMRITTI
GADSPPSRHDADTPAVRSTKAHESKGQEKKAHGSKVHA
>Mature_517_residues
PTKRQQPLKRQRATKIVATVGPASNSEEKLKALFLAGVDVFRLNFSHGTQENHGEVLARIRRLEKEMNRPIGVIADMQGP
KLRIGRFKDGAIAIKAGDILHFDADVDRPGDETRVPIPHPDILAALNVGSTVLCDDGKVRLKVVKKGEGFLEAQVVAGTR
LSNNKGFNIPDVVLPLSPLTDKDRLDLEFACEAGVEWIALSFVQRPEDIHEARALIKDRAAVMLKMEKPSAVDYLTELVE
LSDSIMVARGDLGVEISLAEVPALQKRMIAEARKFGRPVIVATQMLESMITAPVPTRAEVSDVATAVYEGADCVMLSAET
AAGQFPVEAVTFMDDIIRHVECDPGYRQALDATQAEWGKTIGDAVTKATYQAAIAVDASALVAFTLSGTTGLRAARERPA
IPILGLTPKMETARRLALSYGVHAVHMKEDIHSFGDMVDTAVRTAVEHGLGKPGDRLAITAGVPFAKPGTTNIMRITTIG
ADSPPSRHDADTPAVRSTKAHESKGQEKKAHGSKVHA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=36.6598778004073, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=36.6598778004073, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=36.4562118126273, Blast_Score=282, Evalue=7e-76,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.4562118126273, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.4562118126273, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.5853658536585, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.5853658536585, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI310128736, Length=220, Percent_Identity=34.5454545454545, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI310128734, Length=220, Percent_Identity=34.5454545454545, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=31.7535545023697, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=37.6299376299376, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=36.2660944206009, Blast_Score=273, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.4262295081967, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=35.7438016528926, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=35.7438016528926, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=35.7438016528926, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=35.7438016528926, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=511, Percent_Identity=33.8551859099804, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=32.9896907216495, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24648964, Length=496, Percent_Identity=34.8790322580645, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI28571814, Length=496, Percent_Identity=34.8790322580645, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=29.4238683127572, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24648966, Length=442, Percent_Identity=31.6742081447964, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24646914, Length=292, Percent_Identity=33.2191780821918, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 55874; Mature: 55743

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKRQQPLKRQRATKIVATVGPASNSEEKLKALFLAGVDVFRLNFSHGTQENHGEVLAR
CCCCCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
IRRLEKEMNRPIGVIADMQGPKLRIGRFKDGAIAIKAGDILHFDADVDRPGDETRVPIPH
HHHHHHHHCCCEEEEECCCCCCEEEEEECCCEEEEEECCEEEECCCCCCCCCCCCCCCCC
PDILAALNVGSTVLCDDGKVRLKVVKKGEGFLEAQVVAGTRLSNNKGFNIPDVVLPLSPL
CHHHEEECCCCEEEECCCCEEEEEEECCCCCEEEEEEECEEECCCCCCCCCCEEEECCCC
TDKDRLDLEFACEAGVEWIALSFVQRPEDIHEARALIKDRAAVMLKMEKPSAVDYLTELV
CCCCCCCEEEEHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
ELSDSIMVARGDLGVEISLAEVPALQKRMIAEARKFGRPVIVATQMLESMITAPVPTRAE
HCCCCEEEEECCCCEEEEHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCH
VSDVATAVYEGADCVMLSAETAAGQFPVEAVTFMDDIIRHVECDPGYRQALDATQAEWGK
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TIGDAVTKATYQAAIAVDASALVAFTLSGTTGLRAARERPAIPILGLTPKMETARRLALS
HHHHHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
YGVHAVHMKEDIHSFGDMVDTAVRTAVEHGLGKPGDRLAITAGVPFAKPGTTNIMRITTI
HCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEE
GADSPPSRHDADTPAVRSTKAHESKGQEKKAHGSKVHA
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCC
>Mature Secondary Structure 
PTKRQQPLKRQRATKIVATVGPASNSEEKLKALFLAGVDVFRLNFSHGTQENHGEVLAR
CCCCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
IRRLEKEMNRPIGVIADMQGPKLRIGRFKDGAIAIKAGDILHFDADVDRPGDETRVPIPH
HHHHHHHHCCCEEEEECCCCCCEEEEEECCCEEEEEECCEEEECCCCCCCCCCCCCCCCC
PDILAALNVGSTVLCDDGKVRLKVVKKGEGFLEAQVVAGTRLSNNKGFNIPDVVLPLSPL
CHHHEEECCCCEEEECCCCEEEEEEECCCCCEEEEEEECEEECCCCCCCCCCEEEECCCC
TDKDRLDLEFACEAGVEWIALSFVQRPEDIHEARALIKDRAAVMLKMEKPSAVDYLTELV
CCCCCCCEEEEHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
ELSDSIMVARGDLGVEISLAEVPALQKRMIAEARKFGRPVIVATQMLESMITAPVPTRAE
HCCCCEEEEECCCCEEEEHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCH
VSDVATAVYEGADCVMLSAETAAGQFPVEAVTFMDDIIRHVECDPGYRQALDATQAEWGK
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TIGDAVTKATYQAAIAVDASALVAFTLSGTTGLRAARERPAIPILGLTPKMETARRLALS
HHHHHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
YGVHAVHMKEDIHSFGDMVDTAVRTAVEHGLGKPGDRLAITAGVPFAKPGTTNIMRITTI
HCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEE
GADSPPSRHDADTPAVRSTKAHESKGQEKKAHGSKVHA
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA