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The map label for this gene is hisH

Identifier: 154246233

GI number: 154246233

Start: 2556201

End: 2556848

Strand: Reverse

Name: hisH

Synonym: Xaut_2291

Alternate gene names: 154246233

Gene position: 2556848-2556201 (Counterclockwise)

Preceding gene: 154246234

Following gene: 154246232

Centisome position: 48.16

GC content: 68.21

Gene sequence:

>648_bases
ATGAGCGTCGCGATCATCGACTATAATTCCGGCAACCTGCACTCCGCCGCCAAGGCCATGGAGCGCGCCGCGCTTGATCT
CGGCGCGGAACGGGTTGTGGTCACCAGCGATCCGGAGATCGTGCGCACCGCCGACCGGGTGGTTCTGCCGGGCGTCGGCG
CCTTCGCCGACTGTCGCGCCGGCCTCGACGCCGTGCCCGGCATGGTGGAAGCCATGACCGAGGTGGTGAAGGAGCGCGGC
CGCCCCTTCCTCGGCATCTGTGTCGGCCTCCAGCTGCTCGCCGAGCGCGGGCTGGAACACGGGGTGACCGAAGGGCTCGG
CTGGATCCGCGGCGAGGTGGAGCGCATCGCCCCCACCGACCCCGAGCTGAAGATCCCGCACATGGGCTGGAACACCCTCC
AGACCGCGCGCGAACATCCCCTCCTTGCCGGCATTCCCACCGGCGAGGGCGGTTTGCACGCCTATTTCGTGCATTCCTAC
CACCTGAAGCCGGCCGACGGCATCGACCTCGTCGCCACCACGGACTATGGCAGCACCATCACCGCCATGGTCGCGCGCGA
CAACGTGGCCGGCACCCAGTTCCATCCCGAGAAGAGCCAGAGGCTCGGCCTCGCGCTCCTCGCGAATTTCCTGAAGTGGC
ATCCGTGA

Upstream 100 bases:

>100_bases
ATCGGCACGCGCCGACCTGCCGACCATCCGCCCCATGGCATCCCCCGCCTTCGCGGGCGGCCAGGCCGGCGTCATCGGCG
CCTTTCCGGGGACCTGAGCG

Downstream 100 bases:

>100_bases
TCCTTTTTCCGGCCATCGACCTCAAGGACGGCCTCGCCGTGCGCCTCGAACAGGGCGACATGGCGCGGGCCACCGTGTTC
AACCGCGATCCCGCCGCCCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSVAIIDYNSGNLHSAAKAMERAALDLGAERVVVTSDPEIVRTADRVVLPGVGAFADCRAGLDAVPGMVEAMTEVVKERG
RPFLGICVGLQLLAERGLEHGVTEGLGWIRGEVERIAPTDPELKIPHMGWNTLQTAREHPLLAGIPTGEGGLHAYFVHSY
HLKPADGIDLVATTDYGSTITAMVARDNVAGTQFHPEKSQRLGLALLANFLKWHP

Sequences:

>Translated_215_residues
MSVAIIDYNSGNLHSAAKAMERAALDLGAERVVVTSDPEIVRTADRVVLPGVGAFADCRAGLDAVPGMVEAMTEVVKERG
RPFLGICVGLQLLAERGLEHGVTEGLGWIRGEVERIAPTDPELKIPHMGWNTLQTAREHPLLAGIPTGEGGLHAYFVHSY
HLKPADGIDLVATTDYGSTITAMVARDNVAGTQFHPEKSQRLGLALLANFLKWHP
>Mature_214_residues
SVAIIDYNSGNLHSAAKAMERAALDLGAERVVVTSDPEIVRTADRVVLPGVGAFADCRAGLDAVPGMVEAMTEVVKERGR
PFLGICVGLQLLAERGLEHGVTEGLGWIRGEVERIAPTDPELKIPHMGWNTLQTAREHPLLAGIPTGEGGLHAYFVHSYH
LKPADGIDLVATTDYGSTITAMVARDNVAGTQFHPEKSQRLGLALLANFLKWHP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=38.2075471698113, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=34.2342342342342, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23075; Mature: 22944

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIIDYNSGNLHSAAKAMERAALDLGAERVVVTSDPEIVRTADRVVLPGVGAFADCRA
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEECCCCCHHHHHC
GLDAVPGMVEAMTEVVKERGRPFLGICVGLQLLAERGLEHGVTEGLGWIRGEVERIAPTD
CHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCC
PELKIPHMGWNTLQTAREHPLLAGIPTGEGGLHAYFVHSYHLKPADGIDLVATTDYGSTI
CCEECCCCCCHHHHHHHCCCCEEECCCCCCCEEEEEEEEEECCCCCCCEEEEECCCCCEE
TAMVARDNVAGTQFHPEKSQRLGLALLANFLKWHP
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVAIIDYNSGNLHSAAKAMERAALDLGAERVVVTSDPEIVRTADRVVLPGVGAFADCRA
EEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEECCCCCHHHHHC
GLDAVPGMVEAMTEVVKERGRPFLGICVGLQLLAERGLEHGVTEGLGWIRGEVERIAPTD
CHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCC
PELKIPHMGWNTLQTAREHPLLAGIPTGEGGLHAYFVHSYHLKPADGIDLVATTDYGSTI
CCEECCCCCCHHHHHHHCCCCEEECCCCCCCEEEEEEEEEECCCCCCCEEEEECCCCCEE
TAMVARDNVAGTQFHPEKSQRLGLALLANFLKWHP
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA