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The map label for this gene is thiO/thiG [H]

Identifier: 154246166

GI number: 154246166

Start: 2483192

End: 2484397

Strand: Direct

Name: thiO/thiG [H]

Synonym: Xaut_2223

Alternate gene names: 154246166

Gene position: 2483192-2484397 (Clockwise)

Preceding gene: 154246160

Following gene: 154246167

Centisome position: 46.77

GC content: 72.31

Gene sequence:

>1206_bases
ATGAGCGCCAGCTTGTCGACCTTCATGTCCAGTTCTTCTTCAGCCGGCTCGGCAACTCCCGGCGCTGCATCTGCGCGCCC
GCGCGTCGCCATCGCGGGCGGCGGGGTCATCGGCCTCGCTCTTGCTTGGCGGCTTGCGCAGGCGGGCTGCCCGGTGGAGG
TCTTCGAGGCGGGGGAAACCGGCCAGGGCGCGAGCCGTGCCGCCGCCGGCATGCTGGCCGCCTGCGCCGAGGCGGAGCCG
GGGGAGGAGGTTCTCCTCGCCCTCAATCGCGCCAGCCAGGTCCTTTGGCCGGCGTTCGCCGCCGATCTGGAGCAGGCGGC
GGGTTCGTCGGTGGACCTGCGCACCGAAGGCACCCTCACCATCGCCCTCACCGCCGACGATCTCGCGCGGCTGCGGCACC
TTTATGCCCTCCAGCAGTCCCTTGGCCTGCCGGTGGAATGGCTCACGGCGGCGCAGGTGCGGCGGCGCGAGCCCTATCTC
TCGCCCAAGCTGGCCGGCGGCATCCTCACCCTTGAGGACCATCAGGTGGACAACCGCAAGGTGGCCGCCGCGCTCAAGGT
GGCGGCGCTGAAGGCCGGCGTGAATATCCAGGAGCACACGCCCGTCGCGCGGGTGGAGACGGCCGGCGGCCGCGTCACCG
GCCTGGTGGCGGGTGATACGCTCCATGCGGCCGACGTGGTGGTGCTCGCCGCCGGGGCCTGGAGCCGGGGCATCGACATC
ACCCCCGCCGCGCCGCTTCCGGTGCGGCCCATCAAGGGCCAGATGCTGGCGCTGCGCATGGACCCCGCCGCGCCCATCCT
CTCCCATGTGCTGTGGGCGCCGGGGGCCTATCTCGTGCCGCGCCACGACGGCCGCCTCATCATCGGTGCCACCACCGAGG
AGAAGGGCTTCGACACCGATCTCACCGCCGGCGGGCAGCTGGCGCTGCTCACCAATGCGTGGCGCGCCCTGCCGACGCTC
GAGGAGCTGACCATCCTCGAGCAATGGGTCGGCTTCCGCCCCGGCAGCCGGGACGATGCCCCCATCCTCGGTCCGAGCGA
GGAGGTGGACGGCCTGATCTACGCCACCGGCCACCACCGCAACGGCATCCTGCTTCTGCCGGTGACCACGCAGGTGATCG
CCGATTATGTCCTGTCGGGCCGCATGGCGGACGTGGCGGCGCCCTTTGGCGCGGCGCGCTTTGCCCCGCGCGCGGCGGCG
GAGTGA

Upstream 100 bases:

>100_bases
CATGGATAATACCAGAACGGCCTGGGGTGCCACGCATCATGCGCGGCTGAGATCACACCCATCGAACCTGTCTGGGTCAT
TCCAGCGTAGGGAGACGCCG

Downstream 100 bases:

>100_bases
GCGCGATGACGCTCGCCCTGAAGGTGAACGGAACGCCCGAGGACCATCAGGCGGCAACGGTCGCCGACCTGCTGCGCGCC
AAGGGCCTCGATCCCGAGCG

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSASLSTFMSSSSSAGSATPGAASARPRVAIAGGGVIGLALAWRLAQAGCPVEVFEAGETGQGASRAAAGMLAACAEAEP
GEEVLLALNRASQVLWPAFAADLEQAAGSSVDLRTEGTLTIALTADDLARLRHLYALQQSLGLPVEWLTAAQVRRREPYL
SPKLAGGILTLEDHQVDNRKVAAALKVAALKAGVNIQEHTPVARVETAGGRVTGLVAGDTLHAADVVVLAAGAWSRGIDI
TPAAPLPVRPIKGQMLALRMDPAAPILSHVLWAPGAYLVPRHDGRLIIGATTEEKGFDTDLTAGGQLALLTNAWRALPTL
EELTILEQWVGFRPGSRDDAPILGPSEEVDGLIYATGHHRNGILLLPVTTQVIADYVLSGRMADVAAPFGAARFAPRAAA
E

Sequences:

>Translated_401_residues
MSASLSTFMSSSSSAGSATPGAASARPRVAIAGGGVIGLALAWRLAQAGCPVEVFEAGETGQGASRAAAGMLAACAEAEP
GEEVLLALNRASQVLWPAFAADLEQAAGSSVDLRTEGTLTIALTADDLARLRHLYALQQSLGLPVEWLTAAQVRRREPYL
SPKLAGGILTLEDHQVDNRKVAAALKVAALKAGVNIQEHTPVARVETAGGRVTGLVAGDTLHAADVVVLAAGAWSRGIDI
TPAAPLPVRPIKGQMLALRMDPAAPILSHVLWAPGAYLVPRHDGRLIIGATTEEKGFDTDLTAGGQLALLTNAWRALPTL
EELTILEQWVGFRPGSRDDAPILGPSEEVDGLIYATGHHRNGILLLPVTTQVIADYVLSGRMADVAAPFGAARFAPRAAA
E
>Mature_400_residues
SASLSTFMSSSSSAGSATPGAASARPRVAIAGGGVIGLALAWRLAQAGCPVEVFEAGETGQGASRAAAGMLAACAEAEPG
EEVLLALNRASQVLWPAFAADLEQAAGSSVDLRTEGTLTIALTADDLARLRHLYALQQSLGLPVEWLTAAQVRRREPYLS
PKLAGGILTLEDHQVDNRKVAAALKVAALKAGVNIQEHTPVARVETAGGRVTGLVAGDTLHAADVVVLAAGAWSRGIDIT
PAAPLPVRPIKGQMLALRMDPAAPILSHVLWAPGAYLVPRHDGRLIIGATTEEKGFDTDLTAGGQLALLTNAWRALPTLE
ELTILEQWVGFRPGSRDDAPILGPSEEVDGLIYATGHHRNGILLLPVTTQVIADYVLSGRMADVAAPFGAARFAPRAAAE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Homo sapiens, GI194306651, Length=404, Percent_Identity=24.2574257425743, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1787438, Length=410, Percent_Identity=25.8536585365854, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 41580; Mature: 41449

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLSTFMSSSSSAGSATPGAASARPRVAIAGGGVIGLALAWRLAQAGCPVEVFEAGET
CCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCC
GQGASRAAAGMLAACAEAEPGEEVLLALNRASQVLWPAFAADLEQAAGSSVDLRTEGTLT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEE
IALTADDLARLRHLYALQQSLGLPVEWLTAAQVRRREPYLSPKLAGGILTLEDHQVDNRK
EEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCHH
VAAALKVAALKAGVNIQEHTPVARVETAGGRVTGLVAGDTLHAADVVVLAAGAWSRGIDI
HHHHHHHHHHHHCCCHHHCCCCEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCC
TPAAPLPVRPIKGQMLALRMDPAAPILSHVLWAPGAYLVPRHDGRLIIGATTEEKGFDTD
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCC
LTAGGQLALLTNAWRALPTLEELTILEQWVGFRPGSRDDAPILGPSEEVDGLIYATGHHR
CCCCCCEEEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCEEEEEECCCC
NGILLLPVTTQVIADYVLSGRMADVAAPFGAARFAPRAAAE
CCEEEEECHHHHHHHHHHCCCHHHHHCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SASLSTFMSSSSSAGSATPGAASARPRVAIAGGGVIGLALAWRLAQAGCPVEVFEAGET
CCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCC
GQGASRAAAGMLAACAEAEPGEEVLLALNRASQVLWPAFAADLEQAAGSSVDLRTEGTLT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEE
IALTADDLARLRHLYALQQSLGLPVEWLTAAQVRRREPYLSPKLAGGILTLEDHQVDNRK
EEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHCCCEEEEECCCCCCHH
VAAALKVAALKAGVNIQEHTPVARVETAGGRVTGLVAGDTLHAADVVVLAAGAWSRGIDI
HHHHHHHHHHHHCCCHHHCCCCEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCC
TPAAPLPVRPIKGQMLALRMDPAAPILSHVLWAPGAYLVPRHDGRLIIGATTEEKGFDTD
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCC
LTAGGQLALLTNAWRALPTLEELTILEQWVGFRPGSRDDAPILGPSEEVDGLIYATGHHR
CCCCCCEEEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCEEEEEECCCC
NGILLLPVTTQVIADYVLSGRMADVAAPFGAARFAPRAAAE
CCEEEEECHHHHHHHHHHCCCHHHHHCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]