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The map label for this gene is cysW [H]

Identifier: 154246160

GI number: 154246160

Start: 2479035

End: 2479907

Strand: Direct

Name: cysW [H]

Synonym: Xaut_2217

Alternate gene names: 154246160

Gene position: 2479035-2479907 (Clockwise)

Preceding gene: 154246159

Following gene: 154246166

Centisome position: 46.7

GC content: 69.76

Gene sequence:

>873_bases
ATGGCGGCCCCGGTCAACCGCAACCGTCGCCGGGTGGGCGATGGTGCGCGCACGCGGGCGGTGCTGCTCACCCTGGTGCT
CGCCACCACGGCGGTGGCCCTCATCGCGCCGCTCGCGGTCATCTTCTCCGAGGCCTTCGCGCGCGGCTTTTCCGCTTATG
CCGAGGCGCTGGTGCGACCGGACACGCTGCACGCCATCGGCCTCACGATTATCACCGCGCTCGTCTGCGTGCCGGTGAAC
GTCGGCTTCGGCATCGCCGCCGCCTGGGCGGTCACCAAGTTCAGCTTCCCCGGCCGCAACCTGCTGCTGGCGGTGGTGGA
ACTGCCCTTCTCCATCTCGCCCATCGTGGCCGGTGTCGCCTATCTGTTCGTCTATGGCGGGCAGGGGCTGTTCGGCCCGT
TCCTGCAGGCCCACGACATCAAGATCGTGTTCGCCCTGCCGGCCATCATCCTCGCCAGCATGTTCGTCACCGCGCCGTTC
GTGGTGCGCGAGCTGGTGCCGCTGATGATGGCGCAGGGGCGCGACGAGGAAGAGGCGGCGGTGAGCCTGGGCGCCAACGG
CTTCGACGTGCTGCGGCTGGTCACCCTGCCCAACGTGCGCTTCGCCCTGCTCTATGGCGCGGCCTTGTGCAACGCGCGGG
TGATGGGTGAGTTCGGCGCGGTGTCGGTGGTGTCGGGCAACATCCGCGGGCAGACCTCGACCTTGGCCCTGCAGATCGAG
CTTTTGTACCAGGACAGCCACGTCCTCGCCGCCTTCGCCGCCGCGACGGTCCTGGCGGGCGTGGCGCTGTTCACCCTGGT
GGCGAAATATCTCATCGAACGCGCTGAGCGCGCGCGCGAGGAGCGGGCTCCGCCCCGCCGGCCGGGGCACTGA

Upstream 100 bases:

>100_bases
GCCATCGCCGTGACCATGCTGGGGCTCGCCTTCCTTATGCTGCTGGTGGTGAATGCGGTGCAGACGTGGCACCAGCGCAG
TGCGCTGGGGGAGGGCTGAG

Downstream 100 bases:

>100_bases
GGCGGCCGTTTACGGGACTGCGCGCCGCCTCAGTCAGCCGCGCGGGGCGGGCAGGCCGAGGGCGGTGGAGGAGGGCTGCT
GGACGGGTGTCATCCGCTTC

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAAPVNRNRRRVGDGARTRAVLLTLVLATTAVALIAPLAVIFSEAFARGFSAYAEALVRPDTLHAIGLTIITALVCVPVN
VGFGIAAAWAVTKFSFPGRNLLLAVVELPFSISPIVAGVAYLFVYGGQGLFGPFLQAHDIKIVFALPAIILASMFVTAPF
VVRELVPLMMAQGRDEEEAAVSLGANGFDVLRLVTLPNVRFALLYGAALCNARVMGEFGAVSVVSGNIRGQTSTLALQIE
LLYQDSHVLAAFAAATVLAGVALFTLVAKYLIERAERAREERAPPRRPGH

Sequences:

>Translated_290_residues
MAAPVNRNRRRVGDGARTRAVLLTLVLATTAVALIAPLAVIFSEAFARGFSAYAEALVRPDTLHAIGLTIITALVCVPVN
VGFGIAAAWAVTKFSFPGRNLLLAVVELPFSISPIVAGVAYLFVYGGQGLFGPFLQAHDIKIVFALPAIILASMFVTAPF
VVRELVPLMMAQGRDEEEAAVSLGANGFDVLRLVTLPNVRFALLYGAALCNARVMGEFGAVSVVSGNIRGQTSTLALQIE
LLYQDSHVLAAFAAATVLAGVALFTLVAKYLIERAERAREERAPPRRPGH
>Mature_289_residues
AAPVNRNRRRVGDGARTRAVLLTLVLATTAVALIAPLAVIFSEAFARGFSAYAEALVRPDTLHAIGLTIITALVCVPVNV
GFGIAAAWAVTKFSFPGRNLLLAVVELPFSISPIVAGVAYLFVYGGQGLFGPFLQAHDIKIVFALPAIILASMFVTAPFV
VRELVPLMMAQGRDEEEAAVSLGANGFDVLRLVTLPNVRFALLYGAALCNARVMGEFGAVSVVSGNIRGQTSTLALQIEL
LYQDSHVLAAFAAATVLAGVALFTLVAKYLIERAERAREERAPPRRPGH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=265, Percent_Identity=49.811320754717, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788764, Length=209, Percent_Identity=36.3636363636364, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786980, Length=228, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30782; Mature: 30650

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPVNRNRRRVGDGARTRAVLLTLVLATTAVALIAPLAVIFSEAFARGFSAYAEALVRP
CCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTLHAIGLTIITALVCVPVNVGFGIAAAWAVTKFSFPGRNLLLAVVELPFSISPIVAGVA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHH
YLFVYGGQGLFGPFLQAHDIKIVFALPAIILASMFVTAPFVVRELVPLMMAQGRDEEEAA
HHHHCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VSLGANGFDVLRLVTLPNVRFALLYGAALCNARVMGEFGAVSVVSGNIRGQTSTLALQIE
HHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEE
LLYQDSHVLAAFAAATVLAGVALFTLVAKYLIERAERAREERAPPRRPGH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AAPVNRNRRRVGDGARTRAVLLTLVLATTAVALIAPLAVIFSEAFARGFSAYAEALVRP
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTLHAIGLTIITALVCVPVNVGFGIAAAWAVTKFSFPGRNLLLAVVELPFSISPIVAGVA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHH
YLFVYGGQGLFGPFLQAHDIKIVFALPAIILASMFVTAPFVVRELVPLMMAQGRDEEEAA
HHHHCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VSLGANGFDVLRLVTLPNVRFALLYGAALCNARVMGEFGAVSVVSGNIRGQTSTLALQIE
HHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEE
LLYQDSHVLAAFAAATVLAGVALFTLVAKYLIERAERAREERAPPRRPGH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]