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The map label for this gene is mmsB [H]

Identifier: 154246132

GI number: 154246132

Start: 2448339

End: 2449283

Strand: Reverse

Name: mmsB [H]

Synonym: Xaut_2189

Alternate gene names: 154246132

Gene position: 2449283-2448339 (Counterclockwise)

Preceding gene: 154246133

Following gene: 154246112

Centisome position: 46.14

GC content: 70.16

Gene sequence:

>945_bases
GTGGAGAAGGGGGGAAGCCCCCCTGCGGCATTGGGGAGGAACCACATGACCACCATCGCATTCATCGGCCTCGGCAACAT
GGGCGGACCCATGGCGGGCAATCTGGTGAAGGCCGGCCACAGCGTGCTCGGCTTCGACCTCGCCCCGGCGGCGCTGGAGG
GGGCGAAAGCGCGGGGGGTCGTGCTGGCGGACAGTGCCAGGGCGGCCGTGGCGGAAGCCGACGTGGTGGTCACCATGCTG
CCCTCAGGCAAGCATGTGGTTGCCGCCACCAGCGGTTCCGACGGCCTGTTCGCGGCGGCCAGGCCCGGCACCCTGTTCAT
CGACAGCTCCACCATCGACGTGGCCTCCGCCCGCGCCTTCCACGAGGCGGCTAAGACGGCGGGGCATGCCAGCGTGGATG
CCCCCGTCTCCGGCGGCGTGGGCGGGGCGGAAGCGGGCACCCTCACCTTCATGGTGGGCGGCACCGACGACGCCTTCGGC
GCAGCGCGGCCCCTGCTGGAGCTGATGGGCAAGAAGATCGTCCATTGCGGCGGCGCGGGCTCAGGTCAGGCGGCGAAGAT
CTGCAACAACATGATCCTCGGCATCTCGATGATCGCGGTGTCGGAAGCCTTCGTGCTTGGCGAGAAGCTGGGCCTGTCCC
ACCAGGCGCTCTATGACGTGGCCTCCACCTCGTCGGGCCAGTGCTGGTCGCTCACCACCTACTGCCCGGTGCCCGGCCCG
GTGCCCACCAGCCCCGCCAACCGCAACTATGTGCCGGGCTTCGCGGCGGCGCTGATGCTGAAAGACCTGAAGCTGTCGCA
GGACGCCGCCGAGACCTCCGGCGCTGCCACGGCGCTGGGCGCGGCGGCCACCGCCCTCTACACCGCCTTCGCGGCGGAGG
GGAACGAAGGCAAGGATTTCTCCGGCATCGTGGAGTGGATCCGCACCCGCGCCGACGAGACCTGA

Upstream 100 bases:

>100_bases
TCGCGCAGGGGAACTGAGCGGGCCGGCTGGCGCTGCCGCCGGCCGTGGGCAAGTCATTCAGGCCGGAAGAACCGGTCCGC
CCCCCTCTGCCGCTTGGGGG

Downstream 100 bases:

>100_bases
GGGCCGTTTCAAAGATCGTCCGCATTTCCTGCGGACGCTACGGCCAGGCCCGACGCCCTTCAGGCGAAGCAGGCACCGGT
TTGCATGGAAAATGCGACCA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEKGGSPPAALGRNHMTTIAFIGLGNMGGPMAGNLVKAGHSVLGFDLAPAALEGAKARGVVLADSARAAVAEADVVVTML
PSGKHVVAATSGSDGLFAAARPGTLFIDSSTIDVASARAFHEAAKTAGHASVDAPVSGGVGGAEAGTLTFMVGGTDDAFG
AARPLLELMGKKIVHCGGAGSGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALYDVASTSSGQCWSLTTYCPVPGP
VPTSPANRNYVPGFAAALMLKDLKLSQDAAETSGAATALGAAATALYTAFAAEGNEGKDFSGIVEWIRTRADET

Sequences:

>Translated_314_residues
MEKGGSPPAALGRNHMTTIAFIGLGNMGGPMAGNLVKAGHSVLGFDLAPAALEGAKARGVVLADSARAAVAEADVVVTML
PSGKHVVAATSGSDGLFAAARPGTLFIDSSTIDVASARAFHEAAKTAGHASVDAPVSGGVGGAEAGTLTFMVGGTDDAFG
AARPLLELMGKKIVHCGGAGSGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALYDVASTSSGQCWSLTTYCPVPGP
VPTSPANRNYVPGFAAALMLKDLKLSQDAAETSGAATALGAAATALYTAFAAEGNEGKDFSGIVEWIRTRADET
>Mature_314_residues
MEKGGSPPAALGRNHMTTIAFIGLGNMGGPMAGNLVKAGHSVLGFDLAPAALEGAKARGVVLADSARAAVAEADVVVTML
PSGKHVVAATSGSDGLFAAARPGTLFIDSSTIDVASARAFHEAAKTAGHASVDAPVSGGVGGAEAGTLTFMVGGTDDAFG
AARPLLELMGKKIVHCGGAGSGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALYDVASTSSGQCWSLTTYCPVPGP
VPTSPANRNYVPGFAAALMLKDLKLSQDAAETSGAATALGAAATALYTAFAAEGNEGKDFSGIVEWIRTRADET

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=45.2054794520548, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=24.2105263157895, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI145693186, Length=293, Percent_Identity=35.1535836177474, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1786719, Length=298, Percent_Identity=33.5570469798658, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790315, Length=301, Percent_Identity=32.890365448505, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=29.553264604811, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=44.1077441077441, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=43.3898305084746, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=43.3898305084746, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI28574115, Length=294, Percent_Identity=20.7482993197279, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 31319; Mature: 31319

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGGSPPAALGRNHMTTIAFIGLGNMGGPMAGNLVKAGHSVLGFDLAPAALEGAKARGV
CCCCCCCCHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCEEECCCCHHHHCCCCCCCE
VLADSARAAVAEADVVVTMLPSGKHVVAATSGSDGLFAAARPGTLFIDSSTIDVASARAF
EEECCCCHHHEECCEEEEECCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHH
HEAAKTAGHASVDAPVSGGVGGAEAGTLTFMVGGTDDAFGAARPLLELMGKKIVHCGGAG
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEECCCC
SGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALYDVASTSSGQCWSLTTYCPVPGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCC
VPTSPANRNYVPGFAAALMLKDLKLSQDAAETSGAATALGAAATALYTAFAAEGNEGKDF
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCH
SGIVEWIRTRADET
HHHHHHHHHHCCCC
>Mature Secondary Structure
MEKGGSPPAALGRNHMTTIAFIGLGNMGGPMAGNLVKAGHSVLGFDLAPAALEGAKARGV
CCCCCCCCHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCEEECCCCHHHHCCCCCCCE
VLADSARAAVAEADVVVTMLPSGKHVVAATSGSDGLFAAARPGTLFIDSSTIDVASARAF
EEECCCCHHHEECCEEEEECCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHH
HEAAKTAGHASVDAPVSGGVGGAEAGTLTFMVGGTDDAFGAARPLLELMGKKIVHCGGAG
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEECCCC
SGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALYDVASTSSGQCWSLTTYCPVPGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCC
VPTSPANRNYVPGFAAALMLKDLKLSQDAAETSGAATALGAAATALYTAFAAEGNEGKDF
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCH
SGIVEWIRTRADET
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]