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The map label for this gene is kefA [H]

Identifier: 154246037

GI number: 154246037

Start: 2354962

End: 2357544

Strand: Direct

Name: kefA [H]

Synonym: Xaut_2094

Alternate gene names: 154246037

Gene position: 2354962-2357544 (Clockwise)

Preceding gene: 154246033

Following gene: 154246038

Centisome position: 44.36

GC content: 70.58

Gene sequence:

>2583_bases
ATGCATGTAACGAAGCCCGCGGCCGTTCTGGTCGCGCTCTTCCTGTCGATGGTTGTGGCGAGCGTTCCCCTGGCCCAGGC
CGGGGCGCAGACGGCGCAGGGGCAGGCCGCCCCGCACCAGAGCGCGCCCGCCGCGGGCGCGCCGGCTGCGCCTGCGCCCG
TCGCCGGAGCCCCTGCTGCTGCTCCGCCTGCTGCCGCCGCTCCGGAAGCGGCGAAGCCGGTGGTGGTGCCGCCCGATCCC
ATGATCACCGCCGCCCGCGAGAAGCTGGAAGCGGCACGGGCCACCCTCGACGCCCTCGACGCGACGCTGGCGCTGGAGGG
GCTGCGCCCCAACGACCTCGACGACGTGCGCCAGCGGCTCGACCCCGCCCGCCGCGACCTTGCGACGGTGAGCGACCAGA
TCTCCCCGCGCGTCGCCGACACGGAGAGCCGGCTGAGCGGCCTCGGCGCCAAGCCCGCCGAGGGCGCCACCGAGGACCCG
GCGGTGACCGCCGACCGCGAGCGCCTGCAGAAGATCAGCTCCGACCTTGGCGGCGTCCTGAAGCAGAACCGCGCCCTGTT
GCTGCGCGCCGACCAGATCTCCGACCGCATCAGTTCCCGCCGCCGGGCGCTGTTCACCGGCCAGCTGTTCGAGCGCTCGG
ACTCCATCCTCGATCCCGATCTCTGGGGCAACGCGTTGTCCTCGGTGTCGTCCGAGTGGCGGGCCGTCAAGTATTTCGTG
AACGACTGGTCCTCGTTCGCGCAGCGCCGCCAGGGCTCCCTCGCCTTGCTCGGCATCGTGCTGGGCGGCTTCGGCATCGG
CGCCGCTGTGGTGCTGGGCGGGCGTCTGCTGCGCCGCCGGCTGCGCACGCCGCCGCCGGGGTCGGGCGAGAGCTTCACAA
GGCTGCGCTCCGCCCGCGAGGCGCTGAAGGTGCTCGTCCTCAACGCCTGCGTGGCGCCCGCCGCCACCATGGCCGCCCTT
GCCTTTCTGGGCGGGTTCGACGTGGTGCCGCCGCGCGCCGCGGACTTGCTGCACGGGCTGGTGGTGGCGGTCTTCATCAA
GGCCATCGGCAATGCGGTGGCGCGCGCGCTGCTCGCTCCCGACGAGCCGTGGCGCCGCCTGCCGCCGCTCTCGGACGCCG
CCGCAGCGCTGGCTTTCCGCTACTTCTCGTTCGCGGTGTGGACGCTGGCCATCGCCGCCTTCCTCAACGCCCTGCACAAG
GTGCTGTTTGCCCCCGTGGGCCTGACGGTGCTGACCAGCGCGCTGCTTGCCCTGTTCATCGCCGGCTTCATCGCCCGCTT
CCTGGTGGGTGTCGCGCGCTCGGAGGATGGCGGCATCGAGACCGAGGGCGAGGCCTCCCCGGCGGAGCAGGCGGCGTCCG
GCAGCCGCCCGCAATGGCTGCGCCTTGTCATCTGGATGTCGGTGATCCTGCTCATCGGCGCCCTGGTGGGCGGCTATGTG
AGCTTCGCCGCCTTCGTCGCCGCCCGCATGATCGTGGCCGCAGCGGTGCTGGGTGGGCTCTACCTGCTCTACGCCCTCAT
CGATTCCTTCTTCGGCGAGGGGCTTTCGGCCGAGACGCCGCGCGCGCGGCATATGTCCAAGACCCTCGGCCTCCAGCCCA
CCAGCCTCGAGCTGATCGGGGTGGTGCTCTCCGGCCTGCTGAAGCTGCTGCTGTTCGTGGTGGCCGCCTTCCTCATCGCC
GGCAGCTGGGGCACCTCCACCGCCGACGTGATGGACACGGTGGAGCGCGCCTCCTTCGGCGTGCGCATCGGCACCGCCAC
CATCACCCTGTGGAGCGTGTTCTACGCGGTGTTGCTGCTGCTGGTGAGCCTGGTGGCCGCCCGCGCCTTCCAACGCTGGG
TTTCCACCGCCCTGTTGCCGCGCTCGGGGCTGGAGCCGTCGCTGCAATCCTCCATCGCCACCATCGTCAGCTATGTGGGC
ACCATCATCGCCATCATGATCGCCATGAGCGAGGTGGGACTGAACCTGGAGAACATCGCGCTGGTGGCCGGCGCGCTCTC
GGTGGGTATCGGCTTCGGCCTCCAGGCCATCGTGTCCAACTTCGTGTCGGGCCTGATCCTGCTCGCCGAGCGGCCCATAA
GGGTGGGCGACACCATCAATGTGAAGGGCGAGGAGGGCTATGTGCGGCGCATCTCGGTGCGCTCCACCGAGATCGAGACC
TTCGAGCGCGCCACGGTGATCGTGCCCAATTCCGACCTCATCACCGGCATGGTGAAGAACTGGACGCACTCCAACACCAC
CGGCCGCATCATCGTGGCGGTGAACGTGTCCTACGATTGCGATGCGGAGGAGGTGCGCGACATCCTCGTCGGCTGCGCCT
GCGACCATCCGCAGGTGCTGCAGACGCCGCCGCCGCGGGTGTTCCTCACCAAGTTCGCCGATGCGGGCATGGCATTCGAG
CTGCGCTGCGTGGTGGCCAATGTGGACTACGGCCTGACGGTGAAGAGCGACCTGCATTTTCAGGTGCTCGCGCGCTTCCG
CAAGGCCGGCATCGGCATGGCGATCCAGCCCTGGGCCTCGCTGTCGCGCGCGCCCGCCGACATGGTGCCGCCGCCGCCGG
CCCCGCCAGATCCGCTCCAGTGA

Upstream 100 bases:

>100_bases
GGTTTTCGCGATTTCGAAGCCCCGCCGCGCCGCCTTTCTCTCCCATCCGCCACGAACATGCCGCCTTCGGGCGACAGGAA
CGCCGCCGCGGAGGTCCGAT

Downstream 100 bases:

>100_bases
GGGCGGCGAGGGGCTTCGGCTCCCCTCCGCTAACGCTTCTTCAACCCTGAGGGCGCACAAGGCGGGCGGTCCCTCGCGCG
CCGGATCCGCCGAGATGGCG

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MHVTKPAAVLVALFLSMVVASVPLAQAGAQTAQGQAAPHQSAPAAGAPAAPAPVAGAPAAAPPAAAAPEAAKPVVVPPDP
MITAAREKLEAARATLDALDATLALEGLRPNDLDDVRQRLDPARRDLATVSDQISPRVADTESRLSGLGAKPAEGATEDP
AVTADRERLQKISSDLGGVLKQNRALLLRADQISDRISSRRRALFTGQLFERSDSILDPDLWGNALSSVSSEWRAVKYFV
NDWSSFAQRRQGSLALLGIVLGGFGIGAAVVLGGRLLRRRLRTPPPGSGESFTRLRSAREALKVLVLNACVAPAATMAAL
AFLGGFDVVPPRAADLLHGLVVAVFIKAIGNAVARALLAPDEPWRRLPPLSDAAAALAFRYFSFAVWTLAIAAFLNALHK
VLFAPVGLTVLTSALLALFIAGFIARFLVGVARSEDGGIETEGEASPAEQAASGSRPQWLRLVIWMSVILLIGALVGGYV
SFAAFVAARMIVAAAVLGGLYLLYALIDSFFGEGLSAETPRARHMSKTLGLQPTSLELIGVVLSGLLKLLLFVVAAFLIA
GSWGTSTADVMDTVERASFGVRIGTATITLWSVFYAVLLLLVSLVAARAFQRWVSTALLPRSGLEPSLQSSIATIVSYVG
TIIAIMIAMSEVGLNLENIALVAGALSVGIGFGLQAIVSNFVSGLILLAERPIRVGDTINVKGEEGYVRRISVRSTEIET
FERATVIVPNSDLITGMVKNWTHSNTTGRIIVAVNVSYDCDAEEVRDILVGCACDHPQVLQTPPPRVFLTKFADAGMAFE
LRCVVANVDYGLTVKSDLHFQVLARFRKAGIGMAIQPWASLSRAPADMVPPPPAPPDPLQ

Sequences:

>Translated_860_residues
MHVTKPAAVLVALFLSMVVASVPLAQAGAQTAQGQAAPHQSAPAAGAPAAPAPVAGAPAAAPPAAAAPEAAKPVVVPPDP
MITAAREKLEAARATLDALDATLALEGLRPNDLDDVRQRLDPARRDLATVSDQISPRVADTESRLSGLGAKPAEGATEDP
AVTADRERLQKISSDLGGVLKQNRALLLRADQISDRISSRRRALFTGQLFERSDSILDPDLWGNALSSVSSEWRAVKYFV
NDWSSFAQRRQGSLALLGIVLGGFGIGAAVVLGGRLLRRRLRTPPPGSGESFTRLRSAREALKVLVLNACVAPAATMAAL
AFLGGFDVVPPRAADLLHGLVVAVFIKAIGNAVARALLAPDEPWRRLPPLSDAAAALAFRYFSFAVWTLAIAAFLNALHK
VLFAPVGLTVLTSALLALFIAGFIARFLVGVARSEDGGIETEGEASPAEQAASGSRPQWLRLVIWMSVILLIGALVGGYV
SFAAFVAARMIVAAAVLGGLYLLYALIDSFFGEGLSAETPRARHMSKTLGLQPTSLELIGVVLSGLLKLLLFVVAAFLIA
GSWGTSTADVMDTVERASFGVRIGTATITLWSVFYAVLLLLVSLVAARAFQRWVSTALLPRSGLEPSLQSSIATIVSYVG
TIIAIMIAMSEVGLNLENIALVAGALSVGIGFGLQAIVSNFVSGLILLAERPIRVGDTINVKGEEGYVRRISVRSTEIET
FERATVIVPNSDLITGMVKNWTHSNTTGRIIVAVNVSYDCDAEEVRDILVGCACDHPQVLQTPPPRVFLTKFADAGMAFE
LRCVVANVDYGLTVKSDLHFQVLARFRKAGIGMAIQPWASLSRAPADMVPPPPAPPDPLQ
>Mature_860_residues
MHVTKPAAVLVALFLSMVVASVPLAQAGAQTAQGQAAPHQSAPAAGAPAAPAPVAGAPAAAPPAAAAPEAAKPVVVPPDP
MITAAREKLEAARATLDALDATLALEGLRPNDLDDVRQRLDPARRDLATVSDQISPRVADTESRLSGLGAKPAEGATEDP
AVTADRERLQKISSDLGGVLKQNRALLLRADQISDRISSRRRALFTGQLFERSDSILDPDLWGNALSSVSSEWRAVKYFV
NDWSSFAQRRQGSLALLGIVLGGFGIGAAVVLGGRLLRRRLRTPPPGSGESFTRLRSAREALKVLVLNACVAPAATMAAL
AFLGGFDVVPPRAADLLHGLVVAVFIKAIGNAVARALLAPDEPWRRLPPLSDAAAALAFRYFSFAVWTLAIAAFLNALHK
VLFAPVGLTVLTSALLALFIAGFIARFLVGVARSEDGGIETEGEASPAEQAASGSRPQWLRLVIWMSVILLIGALVGGYV
SFAAFVAARMIVAAAVLGGLYLLYALIDSFFGEGLSAETPRARHMSKTLGLQPTSLELIGVVLSGLLKLLLFVVAAFLIA
GSWGTSTADVMDTVERASFGVRIGTATITLWSVFYAVLLLLVSLVAARAFQRWVSTALLPRSGLEPSLQSSIATIVSYVG
TIIAIMIAMSEVGLNLENIALVAGALSVGIGFGLQAIVSNFVSGLILLAERPIRVGDTINVKGEEGYVRRISVRSTEIET
FERATVIVPNSDLITGMVKNWTHSNTTGRIIVAVNVSYDCDAEEVRDILVGCACDHPQVLQTPPPRVFLTKFADAGMAFE
LRCVVANVDYGLTVKSDLHFQVLARFRKAGIGMAIQPWASLSRAPADMVPPPPAPPDPLQ

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=281, Percent_Identity=32.3843416370107, Blast_Score=162, Evalue=9e-41,
Organism=Escherichia coli, GI2367355, Length=308, Percent_Identity=33.1168831168831, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789291, Length=237, Percent_Identity=22.7848101265823, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787591, Length=224, Percent_Identity=26.7857142857143, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 90680; Mature: 90680

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTKPAAVLVALFLSMVVASVPLAQAGAQTAQGQAAPHQSAPAAGAPAAPAPVAGAPAA
CCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPAAAAPEAAKPVVVPPDPMITAAREKLEAARATLDALDATLALEGLRPNDLDDVRQRL
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DPARRDLATVSDQISPRVADTESRLSGLGAKPAEGATEDPAVTADRERLQKISSDLGGVL
CHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KQNRALLLRADQISDRISSRRRALFTGQLFERSDSILDPDLWGNALSSVSSEWRAVKYFV
HCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NDWSSFAQRRQGSLALLGIVLGGFGIGAAVVLGGRLLRRRLRTPPPGSGESFTRLRSARE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ALKVLVLNACVAPAATMAALAFLGGFDVVPPRAADLLHGLVVAVFIKAIGNAVARALLAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEPWRRLPPLSDAAAALAFRYFSFAVWTLAIAAFLNALHKVLFAPVGLTVLTSALLALFI
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AGFIARFLVGVARSEDGGIETEGEASPAEQAASGSRPQWLRLVIWMSVILLIGALVGGYV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFAAFVAARMIVAAAVLGGLYLLYALIDSFFGEGLSAETPRARHMSKTLGLQPTSLELIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
VVLSGLLKLLLFVVAAFLIAGSWGTSTADVMDTVERASFGVRIGTATITLWSVFYAVLLL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHH
LVSLVAARAFQRWVSTALLPRSGLEPSLQSSIATIVSYVGTIIAIMIAMSEVGLNLENIA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAGALSVGIGFGLQAIVSNFVSGLILLAERPIRVGDTINVKGEEGYVRRISVRSTEIET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCEEEEEECHHHHHH
FERATVIVPNSDLITGMVKNWTHSNTTGRIIVAVNVSYDCDAEEVRDILVGCACDHPQVL
HCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
QTPPPRVFLTKFADAGMAFELRCVVANVDYGLTVKSDLHFQVLARFRKAGIGMAIQPWAS
CCCCCHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCCEECCHHH
LSRAPADMVPPPPAPPDPLQ
HCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHVTKPAAVLVALFLSMVVASVPLAQAGAQTAQGQAAPHQSAPAAGAPAAPAPVAGAPAA
CCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPAAAAPEAAKPVVVPPDPMITAAREKLEAARATLDALDATLALEGLRPNDLDDVRQRL
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DPARRDLATVSDQISPRVADTESRLSGLGAKPAEGATEDPAVTADRERLQKISSDLGGVL
CHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KQNRALLLRADQISDRISSRRRALFTGQLFERSDSILDPDLWGNALSSVSSEWRAVKYFV
HCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NDWSSFAQRRQGSLALLGIVLGGFGIGAAVVLGGRLLRRRLRTPPPGSGESFTRLRSARE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ALKVLVLNACVAPAATMAALAFLGGFDVVPPRAADLLHGLVVAVFIKAIGNAVARALLAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEPWRRLPPLSDAAAALAFRYFSFAVWTLAIAAFLNALHKVLFAPVGLTVLTSALLALFI
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AGFIARFLVGVARSEDGGIETEGEASPAEQAASGSRPQWLRLVIWMSVILLIGALVGGYV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFAAFVAARMIVAAAVLGGLYLLYALIDSFFGEGLSAETPRARHMSKTLGLQPTSLELIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
VVLSGLLKLLLFVVAAFLIAGSWGTSTADVMDTVERASFGVRIGTATITLWSVFYAVLLL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHH
LVSLVAARAFQRWVSTALLPRSGLEPSLQSSIATIVSYVGTIIAIMIAMSEVGLNLENIA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LVAGALSVGIGFGLQAIVSNFVSGLILLAERPIRVGDTINVKGEEGYVRRISVRSTEIET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCEEEEEECHHHHHH
FERATVIVPNSDLITGMVKNWTHSNTTGRIIVAVNVSYDCDAEEVRDILVGCACDHPQVL
HCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
QTPPPRVFLTKFADAGMAFELRCVVANVDYGLTVKSDLHFQVLARFRKAGIGMAIQPWAS
CCCCCHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCCEECCHHH
LSRAPADMVPPPPAPPDPLQ
HCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]