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The map label for this gene is proB [H]

Identifier: 154245999

GI number: 154245999

Start: 2315265

End: 2316425

Strand: Direct

Name: proB [H]

Synonym: Xaut_2056

Alternate gene names: 154245999

Gene position: 2315265-2316425 (Clockwise)

Preceding gene: 154245998

Following gene: 154246001

Centisome position: 43.61

GC content: 71.49

Gene sequence:

>1161_bases
ATGACATCGACAGATTCGACATCAGATCCGACGGCTCCGAAGACCCCCGTCTTCACCGACTTCCGCCGCATCGTCATCAA
GGTGGGCTCCGCCCTGCTGGTGGACCGCGACCACGGGCGGGTGAAGCTCGCCTGGCTCACCTCGCTGGTGGAGGACATCG
CGACGCTCCATGCCCGCGGCTGCGAGGTGATGGTGGTGTCTTCGGGCGCTATCGCTTTGGGGCGCACCGTGCTCGGCCTG
CCCAAGGGGCCGCTGAAGCTGGAGGACAGCCAGGCCGCCGCCGCCGTGGGCCAGATCACCCTGTCGCGCACCTGGGCGCA
GATTCTCGGGCATTACGGCATTACCGCCGGCCAGGTGCTGCTGACCCTGGGCGACACCGAGGAGCGCCGGCGCTATCTCA
ACGCCCGCTCCACCCTCGGCCGGCTGATGGACCTCAAGGCGGTACCCATCATCAACGAGAACGACACGGTGGCCACCACC
GAGATCCGCTACGGCGACAACGACCGCCTCGCCGCCCGGGTCGCCACCATGGCCAGCGCCGACCTGCTGGTGCTGCTCTC
GGACATCGACGGGCTCTATACCGCCCCGCCGGCGCAGGACCCCTCCGCCACCCTCATCCCGGTGGTGCCGCGCATCGGCG
CGGAGATCGAGGCCATCGCCGGGGATGCCGGCTCCGAGCTGTCGCGCGGCGGCATGCGCACCAAGATCGAGGCCGGCAAG
ATCGCCACCTCCGCCGGCACCCACATGGTCATCGCCTCCGGCCATGCGAAGAACCCGCTGAAGCGGATCCGGGAGGGCGG
GCGCTGCACCTGGTTCCTCACCTCCGCCAATCCGGTGCGCGCCCGAAAGAGCTGGATCGCCGGGGCGCTGGAGCCGCGCG
GCACGCTGCACCTGGACGACGGGGCCGTGGCGGCGCTGCGCCGGGGCGCCAGCCTTCTGCCGGCCGGGGTGAAACGGGTG
GAAGGCCTGTTCGACCGCGGCGACGCGGTGGTGCTGCGCGGCCCCGACGGCGCCGAGGTGGGCCGGGGGCTGGTGGCCTA
CGACTCCACCCACGCCGCCCGCATCATGGGCCGCTCCACCCACGAGATCGAGGCGCTGCTGGGCGTCCCCGGCCGCTCCG
AGATGGTGCACCGGGACGACATGGCCCTCGGGGTGGAGTGA

Upstream 100 bases:

>100_bases
CCGGGGCGCATGTCGCGCCGCTTCTCTTCAAGGGGGATGACCTCTCACGCACCGGCGCATTCCGCCCGTCCGCCGGCGTG
CTGAAGGTTCCAGAACTTCG

Downstream 100 bases:

>100_bases
GGGGGAGAGCGTCTCAGGGCGAGATCTGCTCCCGCCGTCCGCCAGCGAGGCCCACCTCGACCGCCGGATGGCCTGCGGTC
GCCACCAGGGTGAAATCGGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSTDSTSDPTAPKTPVFTDFRRIVIKVGSALLVDRDHGRVKLAWLTSLVEDIATLHARGCEVMVVSSGAIALGRTVLGL
PKGPLKLEDSQAAAAVGQITLSRTWAQILGHYGITAGQVLLTLGDTEERRRYLNARSTLGRLMDLKAVPIINENDTVATT
EIRYGDNDRLAARVATMASADLLVLLSDIDGLYTAPPAQDPSATLIPVVPRIGAEIEAIAGDAGSELSRGGMRTKIEAGK
IATSAGTHMVIASGHAKNPLKRIREGGRCTWFLTSANPVRARKSWIAGALEPRGTLHLDDGAVAALRRGASLLPAGVKRV
EGLFDRGDAVVLRGPDGAEVGRGLVAYDSTHAARIMGRSTHEIEALLGVPGRSEMVHRDDMALGVE

Sequences:

>Translated_386_residues
MTSTDSTSDPTAPKTPVFTDFRRIVIKVGSALLVDRDHGRVKLAWLTSLVEDIATLHARGCEVMVVSSGAIALGRTVLGL
PKGPLKLEDSQAAAAVGQITLSRTWAQILGHYGITAGQVLLTLGDTEERRRYLNARSTLGRLMDLKAVPIINENDTVATT
EIRYGDNDRLAARVATMASADLLVLLSDIDGLYTAPPAQDPSATLIPVVPRIGAEIEAIAGDAGSELSRGGMRTKIEAGK
IATSAGTHMVIASGHAKNPLKRIREGGRCTWFLTSANPVRARKSWIAGALEPRGTLHLDDGAVAALRRGASLLPAGVKRV
EGLFDRGDAVVLRGPDGAEVGRGLVAYDSTHAARIMGRSTHEIEALLGVPGRSEMVHRDDMALGVE
>Mature_385_residues
TSTDSTSDPTAPKTPVFTDFRRIVIKVGSALLVDRDHGRVKLAWLTSLVEDIATLHARGCEVMVVSSGAIALGRTVLGLP
KGPLKLEDSQAAAAVGQITLSRTWAQILGHYGITAGQVLLTLGDTEERRRYLNARSTLGRLMDLKAVPIINENDTVATTE
IRYGDNDRLAARVATMASADLLVLLSDIDGLYTAPPAQDPSATLIPVVPRIGAEIEAIAGDAGSELSRGGMRTKIEAGKI
ATSAGTHMVIASGHAKNPLKRIREGGRCTWFLTSANPVRARKSWIAGALEPRGTLHLDDGAVAALRRGASLLPAGVKRVE
GLFDRGDAVVLRGPDGAEVGRGLVAYDSTHAARIMGRSTHEIEALLGVPGRSEMVHRDDMALGVE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=31.6151202749141, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=31.8339100346021, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=44.5355191256831, Blast_Score=275, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17569967, Length=254, Percent_Identity=35.0393700787402, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569969, Length=254, Percent_Identity=35.0393700787402, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=33.3333333333333, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321822, Length=413, Percent_Identity=32.4455205811138, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21357643, Length=298, Percent_Identity=33.2214765100671, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40860; Mature: 40729

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTDSTSDPTAPKTPVFTDFRRIVIKVGSALLVDRDHGRVKLAWLTSLVEDIATLHARG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHHHHHHCC
CEVMVVSSGAIALGRTVLGLPKGPLKLEDSQAAAAVGQITLSRTWAQILGHYGITAGQVL
CEEEEEECCCHHHCHHEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LTLGDTEERRRYLNARSTLGRLMDLKAVPIINENDTVATTEIRYGDNDRLAARVATMASA
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCC
DLLVLLSDIDGLYTAPPAQDPSATLIPVVPRIGAEIEAIAGDAGSELSRGGMRTKIEAGK
CEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHCCCCEEEEECCC
IATSAGTHMVIASGHAKNPLKRIREGGRCTWFLTSANPVRARKSWIAGALEPRGTLHLDD
EEECCCCEEEEECCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECC
GAVAALRRGASLLPAGVKRVEGLFDRGDAVVLRGPDGAEVGRGLVAYDSTHAARIMGRST
CHHHHHHCCCHHCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEEECCCHHHHHHCCCH
HEIEALLGVPGRSEMVHRDDMALGVE
HHHHHHHCCCCCCCCEECCCEEECCC
>Mature Secondary Structure 
TSTDSTSDPTAPKTPVFTDFRRIVIKVGSALLVDRDHGRVKLAWLTSLVEDIATLHARG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHHHHHHHHHCC
CEVMVVSSGAIALGRTVLGLPKGPLKLEDSQAAAAVGQITLSRTWAQILGHYGITAGQVL
CEEEEEECCCHHHCHHEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LTLGDTEERRRYLNARSTLGRLMDLKAVPIINENDTVATTEIRYGDNDRLAARVATMASA
EEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCC
DLLVLLSDIDGLYTAPPAQDPSATLIPVVPRIGAEIEAIAGDAGSELSRGGMRTKIEAGK
CEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHCCCCEEEEECCC
IATSAGTHMVIASGHAKNPLKRIREGGRCTWFLTSANPVRARKSWIAGALEPRGTLHLDD
EEECCCCEEEEECCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEECC
GAVAALRRGASLLPAGVKRVEGLFDRGDAVVLRGPDGAEVGRGLVAYDSTHAARIMGRST
CHHHHHHCCCHHCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEEECCCHHHHHHCCCH
HEIEALLGVPGRSEMVHRDDMALGVE
HHHHHHHCCCCCCCCEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA