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The map label for this gene is proA

Identifier: 154246001

GI number: 154246001

Start: 2317448

End: 2318821

Strand: Direct

Name: proA

Synonym: Xaut_2058

Alternate gene names: 154246001

Gene position: 2317448-2318821 (Clockwise)

Preceding gene: 154245999

Following gene: 154246002

Centisome position: 43.65

GC content: 70.74

Gene sequence:

>1374_bases
GTGAGGCATCATCGTCTTACCCCTCAGCGAGACCCTGCCATGGCCACCGCCTCCGCCGCCCGTACCGATGTTTCCGCCGT
CGCGGGCCGGTCCGTGCCGCCGGTGGAAGGCGGGGCGGATCTTGCCGCTTATATGAGCGACATGGGCCGGCGCGCCCGTG
CCGCCTCCCGTACCCTCGCCCTCACCCCGCCCGAGGCGAAGACGCAGGCGCTCCATGCCGCCGCCGCCGCCATCCGCGCC
GCTGCCGACGAGATCCTCGCGGCCAATGCCGGCGACGTGGCCAAGGCGAAGGCGGACGGCATGACCGGCTCGTTCCTCGA
CCGCCTCACCCTCACCCCCGCCCGCATCGCGGCCATGGCCGAGGGCGTGGAGCAGGTGGCGGCCCTTTCCGATCCCGTGG
GCGAGGTGACCGAGAGCTGGACCCGCCCCAACGGCTTGAAAATCGAGCGCGTGCGCACGCCGCTGGGCGTCATCGGCGTC
ATCTATGAGAGCCGGCCCAATGTGACGGCGGACGCCGCCGCTCTGTGCCTGCGCGCCGGCAACGCCGTGATCCTGCGCGG
CGGCTCCGACAGCATCGCCTCGTCCGGCGCCATCCATCGCGCCTTCGTCTCCGGCCTCAAGTCCGCCGGCCTGCCGGAGG
ATGCGGTGCAGTTCGTCGCCGTGGCCGATCGCGCGGCGGTGGGGCTGATGCTGGCCGGGCTCGACAATAATATGGATGTC
ATCGTGCCGCGTGGCGGCAAGAGCCTGGTGGCACGGGTGCAGAGCGACGCGAGGGTGCCGGTGTTCGCCCATCTGGACGG
CATCAACCACGTGTATGTGGACAAGGCCGCCGACCTCGACATGGCCAAGACCGTGGTGCTGAACGCCAAGATGCGCCGCA
CCGGCGTCTGCGGCGCCGCCGAGACCCTGCTGGTGGACCAGGCGGTGGCCGCCACCCACCTGAAGCCGCTGGTGGAAATG
CTCATCGACGCCGGCTGCGAGGTGCGCGGCGACGAGGCCGTGCAGGCGGTTGATGGGCGGGTTAAGCCCGCCACGCCGCT
GGACTGGGACACGGAATATCTCGACGCCATCATCGCCGCCAAGGTGGTGGACGGGCTGGCCGGAGCCGTCGCCCATATCG
AGGCCCACGGCTCCCACCACACGGACTCCATCGTCACCGAGGACGTGGCGACGGCGGAGCGCTTCCTGGCGGAGGTGGAT
TCCGCCATCGTGCTCCACAATGCCTCCACCCAGTTCGCGGACGGCGGGGAGTTCGGGTTCGGCGGGGAGATCGGCATCGC
CACCGGGCGCATGCATGCCCGCGGCCCGGTGGGCGCGGAACAGCTCACCTCGTTCAAATACCGCATCCACGGCTCGGGGC
AGACCCGTCCGTGA

Upstream 100 bases:

>100_bases
GCCGCTGCTGACAATGCCGCGAGTGGGTCCCTCCGGTACCCTGCTCCGGTCTGTCGCATTTCGCCTCTGACGTGCTAATC
AGGGTGGTGATGCCTATCTT

Downstream 100 bases:

>100_bases
GCTTTCCACCCGCCAGCGGGAGGGCGCGGGACGGCGGCGCGGTGAGCCCCCGCGCCGCGCGCCACATGCCGTGGGTGACC
GACGGCCTGCGCGTGGGCCT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRHHRLTPQRDPAMATASAARTDVSAVAGRSVPPVEGGADLAAYMSDMGRRARAASRTLALTPPEAKTQALHAAAAAIRA
AADEILAANAGDVAKAKADGMTGSFLDRLTLTPARIAAMAEGVEQVAALSDPVGEVTESWTRPNGLKIERVRTPLGVIGV
IYESRPNVTADAAALCLRAGNAVILRGGSDSIASSGAIHRAFVSGLKSAGLPEDAVQFVAVADRAAVGLMLAGLDNNMDV
IVPRGGKSLVARVQSDARVPVFAHLDGINHVYVDKAADLDMAKTVVLNAKMRRTGVCGAAETLLVDQAVAATHLKPLVEM
LIDAGCEVRGDEAVQAVDGRVKPATPLDWDTEYLDAIIAAKVVDGLAGAVAHIEAHGSHHTDSIVTEDVATAERFLAEVD
SAIVLHNASTQFADGGEFGFGGEIGIATGRMHARGPVGAEQLTSFKYRIHGSGQTRP

Sequences:

>Translated_457_residues
MRHHRLTPQRDPAMATASAARTDVSAVAGRSVPPVEGGADLAAYMSDMGRRARAASRTLALTPPEAKTQALHAAAAAIRA
AADEILAANAGDVAKAKADGMTGSFLDRLTLTPARIAAMAEGVEQVAALSDPVGEVTESWTRPNGLKIERVRTPLGVIGV
IYESRPNVTADAAALCLRAGNAVILRGGSDSIASSGAIHRAFVSGLKSAGLPEDAVQFVAVADRAAVGLMLAGLDNNMDV
IVPRGGKSLVARVQSDARVPVFAHLDGINHVYVDKAADLDMAKTVVLNAKMRRTGVCGAAETLLVDQAVAATHLKPLVEM
LIDAGCEVRGDEAVQAVDGRVKPATPLDWDTEYLDAIIAAKVVDGLAGAVAHIEAHGSHHTDSIVTEDVATAERFLAEVD
SAIVLHNASTQFADGGEFGFGGEIGIATGRMHARGPVGAEQLTSFKYRIHGSGQTRP
>Mature_457_residues
MRHHRLTPQRDPAMATASAARTDVSAVAGRSVPPVEGGADLAAYMSDMGRRARAASRTLALTPPEAKTQALHAAAAAIRA
AADEILAANAGDVAKAKADGMTGSFLDRLTLTPARIAAMAEGVEQVAALSDPVGEVTESWTRPNGLKIERVRTPLGVIGV
IYESRPNVTADAAALCLRAGNAVILRGGSDSIASSGAIHRAFVSGLKSAGLPEDAVQFVAVADRAAVGLMLAGLDNNMDV
IVPRGGKSLVARVQSDARVPVFAHLDGINHVYVDKAADLDMAKTVVLNAKMRRTGVCGAAETLLVDQAVAATHLKPLVEM
LIDAGCEVRGDEAVQAVDGRVKPATPLDWDTEYLDAIIAAKVVDGLAGAVAHIEAHGSHHTDSIVTEDVATAERFLAEVD
SAIVLHNASTQFADGGEFGFGGEIGIATGRMHARGPVGAEQLTSFKYRIHGSGQTRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=444, Percent_Identity=36.2612612612613, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI62912457, Length=444, Percent_Identity=36.2612612612613, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1786438, Length=407, Percent_Identity=42.5061425061425, Blast_Score=323, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17569969, Length=431, Percent_Identity=35.7308584686775, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17569967, Length=431, Percent_Identity=35.7308584686775, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=36.2790697674419, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI21357643, Length=408, Percent_Identity=36.0294117647059, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 47359; Mature: 47359

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHHRLTPQRDPAMATASAARTDVSAVAGRSVPPVEGGADLAAYMSDMGRRARAASRTLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
LTPPEAKTQALHAAAAAIRAAADEILAANAGDVAKAKADGMTGSFLDRLTLTPARIAAMA
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHH
EGVEQVAALSDPVGEVTESWTRPNGLKIERVRTPLGVIGVIYESRPNVTADAAALCLRAG
HHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCC
NAVILRGGSDSIASSGAIHRAFVSGLKSAGLPEDAVQFVAVADRAAVGLMLAGLDNNMDV
CEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEE
IVPRGGKSLVARVQSDARVPVFAHLDGINHVYVDKAADLDMAKTVVLNAKMRRTGVCGAA
EEECCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCHHHHCCCCCHH
ETLLVDQAVAATHLKPLVEMLIDAGCEVRGDEAVQAVDGRVKPATPLDWDTEYLDAIIAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KVVDGLAGAVAHIEAHGSHHTDSIVTEDVATAERFLAEVDSAIVLHNASTQFADGGEFGF
HHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
GGEIGIATGRMHARGPVGAEQLTSFKYRIHGSGQTRP
CCCEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure
MRHHRLTPQRDPAMATASAARTDVSAVAGRSVPPVEGGADLAAYMSDMGRRARAASRTLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
LTPPEAKTQALHAAAAAIRAAADEILAANAGDVAKAKADGMTGSFLDRLTLTPARIAAMA
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHH
EGVEQVAALSDPVGEVTESWTRPNGLKIERVRTPLGVIGVIYESRPNVTADAAALCLRAG
HHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCC
NAVILRGGSDSIASSGAIHRAFVSGLKSAGLPEDAVQFVAVADRAAVGLMLAGLDNNMDV
CEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEE
IVPRGGKSLVARVQSDARVPVFAHLDGINHVYVDKAADLDMAKTVVLNAKMRRTGVCGAA
EEECCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCHHHHCCCCCHH
ETLLVDQAVAATHLKPLVEMLIDAGCEVRGDEAVQAVDGRVKPATPLDWDTEYLDAIIAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KVVDGLAGAVAHIEAHGSHHTDSIVTEDVATAERFLAEVDSAIVLHNASTQFADGGEFGF
HHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
GGEIGIATGRMHARGPVGAEQLTSFKYRIHGSGQTRP
CCCEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]