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The map label for this gene is rpoH [H]

Identifier: 154245929

GI number: 154245929

Start: 2243284

End: 2244186

Strand: Direct

Name: rpoH [H]

Synonym: Xaut_1986

Alternate gene names: 154245929

Gene position: 2243284-2244186 (Clockwise)

Preceding gene: 154245928

Following gene: 154245932

Centisome position: 42.25

GC content: 66.45

Gene sequence:

>903_bases
ATGGCCCAGCCGAAGTCCATGTCCATTCCGTCCGCCGAAGGCGGGCTCTCCCGGTATCTTGAAGAGATCCGGCGGTTTCC
CATGCTGGAGCCGCAGGAGGAATATATGCTCGCCAAGAGCTGGCGCGAGCACGGGGACCGCGATGCCGCGCACAGGCTGG
TGACGAGCCACCTGCGCCTCGTGGCGAAGATCGCCATGGGCTACCGCGGTTATGGGCTGCCCATCTCCGAGGTGATTTCC
GAGGGCAACGTGGGCCTCATGCAGGCGGTGAAGCGGTTCGAGCCCGAGAAGGGCTTCCGCCTCGCCACCTATGCCATGTG
GTGGATCCGCGCCTCCATCCAGGAATATATCCTGCGCTCTTGGAGCCTCGTGAAGATGGGCACCACGGCGGCGCAGAAGA
AGCTGTTCTTCAACCTGCGCAAGGCCAAGAGCCAGATCTCGGCGCTGGAGGACGGCGACCTCAAGCCCGAGCACGTCAAG
CAGATCGCCACCAAGCTCGGCGTCACCGAGCAGGACGTGGTGGACATGAACCGCCGCCTGTCCGGCGATGCCTCCCTGAA
CTCCCCCATCCGGGAGGATTCCGAGGGCGGGGAGTGGCAGGACTGGCTGGTGGACGAGCGCCTCGACCAGGAGGAGCAAC
TCGCCGAGAGCGAGGAGCTGGACAATCGCCGCGCCGCGTTGTCGGGCGCTCTGTCGGTGCTGAATGACCGCGAGCGCCGC
ATTTTCGAGGCCCGCCGCCTCTCCGAGGATCCCATGACCCTGGAGGACCTCGCCTCCGAGTTCGGCGTCTCCCGCGAGCG
GGTGCGCCAGATCGAGGTGCGCGCCTTCGAGAAGGTGCAGAAGGCGGTCATGGACAAGGTCCGCCAGGTCGAGGCCCCGG
AGGCGGAAGCCGTGGGGGCGTGA

Upstream 100 bases:

>100_bases
GCATTCCGCTCGTATCTATATCATGATGCGTCGCGGTCGGCGTCATGCGGCCGCTGAGGCGGTCGCCCGGACGGGGGCCG
CGGGAGAAGGAGGCCTCGTC

Downstream 100 bases:

>100_bases
GGTCGGCTGTCGAAGTTGCCAATCCGGCAGAATGAAGAGGGACGGTGTCCGATGCCGTCCCCTTTTTGCGGTGTCATGCG
CGGGGGCCTCGGTGGGATCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAQPKSMSIPSAEGGLSRYLEEIRRFPMLEPQEEYMLAKSWREHGDRDAAHRLVTSHLRLVAKIAMGYRGYGLPISEVIS
EGNVGLMQAVKRFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQISALEDGDLKPEHVK
QIATKLGVTEQDVVDMNRRLSGDASLNSPIREDSEGGEWQDWLVDERLDQEEQLAESEELDNRRAALSGALSVLNDRERR
IFEARRLSEDPMTLEDLASEFGVSRERVRQIEVRAFEKVQKAVMDKVRQVEAPEAEAVGA

Sequences:

>Translated_300_residues
MAQPKSMSIPSAEGGLSRYLEEIRRFPMLEPQEEYMLAKSWREHGDRDAAHRLVTSHLRLVAKIAMGYRGYGLPISEVIS
EGNVGLMQAVKRFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQISALEDGDLKPEHVK
QIATKLGVTEQDVVDMNRRLSGDASLNSPIREDSEGGEWQDWLVDERLDQEEQLAESEELDNRRAALSGALSVLNDRERR
IFEARRLSEDPMTLEDLASEFGVSRERVRQIEVRAFEKVQKAVMDKVRQVEAPEAEAVGA
>Mature_299_residues
AQPKSMSIPSAEGGLSRYLEEIRRFPMLEPQEEYMLAKSWREHGDRDAAHRLVTSHLRLVAKIAMGYRGYGLPISEVISE
GNVGLMQAVKRFEPEKGFRLATYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRKAKSQISALEDGDLKPEHVKQ
IATKLGVTEQDVVDMNRRLSGDASLNSPIREDSEGGEWQDWLVDERLDQEEQLAESEELDNRRAALSGALSVLNDRERRI
FEARRLSEDPMTLEDLASEFGVSRERVRQIEVRAFEKVQKAVMDKVRQVEAPEAEAVGA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=42.7536231884058, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=34.6570397111913, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=30.8016877637131, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34208; Mature: 34077

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPKSMSIPSAEGGLSRYLEEIRRFPMLEPQEEYMLAKSWREHGDRDAAHRLVTSHLRL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPISEVISEGNVGLMQAVKRFEPEKGFRLATYAMWWIRASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
WSLVKMGTTAAQKKLFFNLRKAKSQISALEDGDLKPEHVKQIATKLGVTEQDVVDMNRRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
SGDASLNSPIREDSEGGEWQDWLVDERLDQEEQLAESEELDNRRAALSGALSVLNDRERR
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFEARRLSEDPMTLEDLASEFGVSRERVRQIEVRAFEKVQKAVMDKVRQVEAPEAEAVGA
HHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
AQPKSMSIPSAEGGLSRYLEEIRRFPMLEPQEEYMLAKSWREHGDRDAAHRLVTSHLRL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]