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The map label for this gene is puuA [H]

Identifier: 154245882

GI number: 154245882

Start: 2194995

End: 2196398

Strand: Direct

Name: puuA [H]

Synonym: Xaut_1939

Alternate gene names: 154245882

Gene position: 2194995-2196398 (Clockwise)

Preceding gene: 154245881

Following gene: 154245883

Centisome position: 41.35

GC content: 66.24

Gene sequence:

>1404_bases
ATGGCGAAACGCAGCAAGGGCGCGCAGAAGATTCCGGTGACGGCGCCGCGGGGTGTCAATTCCCTCGCCGAGGCCAAGGC
CTGGATGGCCAGCCGGGGCATCACCGAGATCGAGTGCACCGTTCCCGATCTTGCGGGTGTCGCCCGCGGCAAGATCATGC
CGGCCTCGAAATTCTTCTCCTCCCCGGTGATGAACCTGCCCCTGTCGGTCTTCTTCCAGACCATTTCGGGCGAGTATCCG
GACTATGAGGGCCTGGTGGATTCGGTGGTCGCCGACAGCGACCTGGTGATGGAGCCGGACCTCGCCACCCTGTGCTCGGT
GCCGTGGGCGCAGGACCCCACCGCCCAGGTGATCCACGACGCCTTCCACCGCGACGGGCGTCCGGTGGAGCTGGCGCCGC
GGCAGGTGCTGCGCCACGTCATCTCGCTCTACGAGAAGAAGGGCTGGAAGCCGGTGGTGGCGCCGGAAATCGAATTCTAC
CTGGTGGAGCCCAACACCGATCCCGACTATCCGCTGAAGCCGCCGGTGGGCCGCTCGGGGCGTCCCGAGATCGGCCGCCA
GTCCTATTCCATCCAGGCGGTGAACGAGTTCGATGCCCTGTTCGAGGACATCTACGACTATTCGGAAGCCCAGGGGCTGG
AGATCGACACGCTGATCCACGAGGACGGCGCCGCGCAGATGGAAATCAACCTGCGCCACGGCGATCCGCTCACGCTGGCC
GACCAGGTGTTCCTGTTCAAGCGCACCATCCGCGAGGCGGCGCTGGCGCACAAGATCTACGCCACCTTCATGGCCAAGCC
CATCGCCAACGAGCCGGGCAGCGCCATGCACATCCACCAGTCGGTGATCTCGGCGCAGACCGGCAAGAACATCTTCTCGG
ACGAGGACGGCGACCCGACGCCGGAATTCTTCTCCTTCATCGCCGGCCACCAGAAATACCTGCCGTCGGTCATGTGCATC
ATGGCGCCCTATGTGAATTCCTACCGGCGGCTGACGCGCGACTCCATGGCGCCCATCAACCTGCAATGGGGCTACGACAA
CCGCACCGCAGGCCTGCGCGTGCCGCCCTCCACCCCCGAGGCGCGGCGGCTGGAGAACCGGGTGCCGTCCTCCGACGCCA
ATCCCTATCTCGTCATCGCCGCCTCCCTGGCCTGCGGCTATCTCGGCCTCACCGAGAACCTGCGCGCCACCGATCCGCTG
GAGGGCGACGCCAAGAACCTCGACTTCGACCTGCCGCGCGGCCTCCTGGAGGCGGTGGCGGCCTTCTCGGACAGCGAGCC
CCTCGCCGAGGTGCTCGGCCATCGCTTCGTTGCCGCCTATGCCGCGGTGAAGCAGGCCGAGTTCGAGACCTTCATGCGGG
TGATCTCGCCGTGGGAGCGGGAATATCTGCTGCTGAACGTCTGA

Upstream 100 bases:

>100_bases
GGGATGCAGCGGGAGCGCGGCCGCGACGGCGCCGCGTTGACACCCGCCATGGCGCAGGTGTTCCCTCATCAGGCGTGAGC
GGTTCACGCGTGAGGGCATC

Downstream 100 bases:

>100_bases
GCGCGCGCATGGCAGGCGAGGCTCAATCCAGGCCGCCGACGGGGCTCGATCCCGCCGGGCACGCGCCGTCCTGGTACGCG
GCGACGGCCATCGCCTGTCC

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAKRSKGAQKIPVTAPRGVNSLAEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFSSPVMNLPLSVFFQTISGEYP
DYEGLVDSVVADSDLVMEPDLATLCSVPWAQDPTAQVIHDAFHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFY
LVEPNTDPDYPLKPPVGRSGRPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLTLA
DQVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVISAQTGKNIFSDEDGDPTPEFFSFIAGHQKYLPSVMCI
MAPYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPEARRLENRVPSSDANPYLVIAASLACGYLGLTENLRATDPL
EGDAKNLDFDLPRGLLEAVAAFSDSEPLAEVLGHRFVAAYAAVKQAEFETFMRVISPWEREYLLLNV

Sequences:

>Translated_467_residues
MAKRSKGAQKIPVTAPRGVNSLAEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFSSPVMNLPLSVFFQTISGEYP
DYEGLVDSVVADSDLVMEPDLATLCSVPWAQDPTAQVIHDAFHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFY
LVEPNTDPDYPLKPPVGRSGRPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLTLA
DQVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVISAQTGKNIFSDEDGDPTPEFFSFIAGHQKYLPSVMCI
MAPYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPEARRLENRVPSSDANPYLVIAASLACGYLGLTENLRATDPL
EGDAKNLDFDLPRGLLEAVAAFSDSEPLAEVLGHRFVAAYAAVKQAEFETFMRVISPWEREYLLLNV
>Mature_466_residues
AKRSKGAQKIPVTAPRGVNSLAEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFSSPVMNLPLSVFFQTISGEYPD
YEGLVDSVVADSDLVMEPDLATLCSVPWAQDPTAQVIHDAFHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFYL
VEPNTDPDYPLKPPVGRSGRPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLTLAD
QVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVISAQTGKNIFSDEDGDPTPEFFSFIAGHQKYLPSVMCIM
APYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPEARRLENRVPSSDANPYLVIAASLACGYLGLTENLRATDPLE
GDAKNLDFDLPRGLLEAVAAFSDSEPLAEVLGHRFVAAYAAVKQAEFETFMRVISPWEREYLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=456, Percent_Identity=25, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081870, Length=458, Percent_Identity=34.4978165938865, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790301, Length=358, Percent_Identity=27.9329608938547, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51715; Mature: 51584

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRSKGAQKIPVTAPRGVNSLAEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCHHHHHC
SPVMNLPLSVFFQTISGEYPDYEGLVDSVVADSDLVMEPDLATLCSVPWAQDPTAQVIHD
CCHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCHHHHHCCCCCCCCHHHHHHH
AFHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFYLVEPNTDPDYPLKPPVGRSG
HHHCCCCCEECCHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCC
RPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLTLA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCHHH
DQVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVISAQTGKNIFSDEDGDPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
PEFFSFIAGHQKYLPSVMCIMAPYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCH
ARRLENRVPSSDANPYLVIAASLACGYLGLTENLRATDPLEGDAKNLDFDLPRGLLEAVA
HHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AFSDSEPLAEVLGHRFVAAYAAVKQAEFETFMRVISPWEREYLLLNV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
AKRSKGAQKIPVTAPRGVNSLAEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCHHHHHC
SPVMNLPLSVFFQTISGEYPDYEGLVDSVVADSDLVMEPDLATLCSVPWAQDPTAQVIHD
CCHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCHHHHHCCCCCCCCHHHHHHH
AFHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFYLVEPNTDPDYPLKPPVGRSG
HHHCCCCCEECCHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCC
RPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLTLA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCCHHH
DQVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVISAQTGKNIFSDEDGDPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
PEFFSFIAGHQKYLPSVMCIMAPYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCH
ARRLENRVPSSDANPYLVIAASLACGYLGLTENLRATDPLEGDAKNLDFDLPRGLLEAVA
HHHHHHCCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AFSDSEPLAEVLGHRFVAAYAAVKQAEFETFMRVISPWEREYLLLNV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]