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The map label for this gene is ispG

Identifier: 154245833

GI number: 154245833

Start: 2128068

End: 2129414

Strand: Direct

Name: ispG

Synonym: Xaut_1889

Alternate gene names: 154245833

Gene position: 2128068-2129414 (Clockwise)

Preceding gene: 154245832

Following gene: 154245834

Centisome position: 40.08

GC content: 70.3

Gene sequence:

>1347_bases
ATGCCCATGACCACCCTTCAGTCCACGCCTGCGACCCAGGTTCCCCCGGCCACCACTGCCGTGGTCCCCGCCGAGCTGCT
TGAGGCGGGTCCCGCCGCGCGGCGTCCGACGGTGGGCGTTCCGGTGGGCCGGGTGATGGTGGGCGGCGGCGCGCCGGTGG
TGGTGCAGTCCATGACCAACACCGACACCGCCGACATCGAGGGCACCGTGCGCCAGGTGGCCGCGCTGGCCCGCGCCGGC
TCGGAAATCGTGCGCATCACGGTGGACCGCAACGAGGCCGCCGCCGCCGTGCCGCACATCAAGGAAAAGCTGCTGGCGCA
GGGCATCGACGTGCCGCTGGTGGGCGATTTCCATTACATCGGCCACAAGCTGCTGGCCGATTATCCGGCCTGCGCCGAGG
CGCTCGACAAATACCGCATCAATCCGGGCAACGTGGGCTTCGGCGACAAGAAGGACCGGCAGTTCGCCGCCATCGTCGAG
ACCGCCATCCGCCACGACAAGCCGGTGCGCATCGGCGCCAACTGGGGCTCCCTCGATGGTGAGCTGCTGACCCGCCTGAT
GGACGAGAACGCCTTGCTGCCACAGCCGGCGGAAGCCCGCGCCGTGACCCGCGAGGCGCTGGTGCGCTCGGCCCTGCTCT
CGGCGGGGCTGGCGGAGGAGATCGGCCTTCCCCGCAACCGCATCATCCTCTCGGCCAAGGTCTCGGCGGTGCAGGACCTC
ATCGCCGTCTATGGCGAGCTGTCCCGCCGCGCCGACTATGCGCTGCATCTCGGCCTGACCGAGGCGGGCATGGGCTCGAA
GGGCATCGTTGCTTCCACGGCGGCCATGGGGGTGGTGTTGCAGGCCGGCATCGGCGACACCATCCGCGTCTCCCTGACCC
CGGAGCCCAACGGCGATCGCACCCGCGAGGTGCAGGTGGCGCAGGAAATACTGCAGACCATGGGCCTGCGCACCTTCGTG
CCGCTGGTGGCCGCCTGCCCCGGCTGCGGGCGCACCACCTCCACCGTGTTCCAGGAGCTGGCGCAGAGCGTGCAGGAGAT
GATCCGCACCCGCATGCCGGTCTGGAAGGCGAAATTTCCCGGCGTCGAGACCCTGAACGTGGCGGTGATGGGCTGCATCG
TGAACGGCCCGGGAGAGAGCAAGCATGCCGACATCGGCATCTCCCTGCCCGGCACCGGGGAGACCCCGTCCGCCCCGGTC
TTCATCGACGGCAAGAAGGTGGCGACCCTGCGCGGCGCCAACATCCAGGACGAGTTCCGCCGGATGGTGGAGGCCTATGT
GGAGCAGCGCTTCGGCGTCGGCGGCACGGCGGCGGCAGGGGCATCCCCGGCGGACGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GGCCTACTCGAAACCCGTCCGTGCCCCCTCCCGCGCCGTATCAGGGCCGGACGTGCCGGTCGCGGACGTGTCTGCGTGCG
GACGATTTGCCGAGGATGGA

Downstream 100 bases:

>100_bases
GGCGAAGGAAGCCGTGAGCCGGCCCATGGGCCTTCGGCCAAGCCCGCGCGGCGCCTGAGCGTGACCCACCTGGCGCCGGT
CGAAGACCGGGGCCGATGGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPMTTLQSTPATQVPPATTAVVPAELLEAGPAARRPTVGVPVGRVMVGGGAPVVVQSMTNTDTADIEGTVRQVAALARAG
SEIVRITVDRNEAAAAVPHIKEKLLAQGIDVPLVGDFHYIGHKLLADYPACAEALDKYRINPGNVGFGDKKDRQFAAIVE
TAIRHDKPVRIGANWGSLDGELLTRLMDENALLPQPAEARAVTREALVRSALLSAGLAEEIGLPRNRIILSAKVSAVQDL
IAVYGELSRRADYALHLGLTEAGMGSKGIVASTAAMGVVLQAGIGDTIRVSLTPEPNGDRTREVQVAQEILQTMGLRTFV
PLVAACPGCGRTTSTVFQELAQSVQEMIRTRMPVWKAKFPGVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPV
FIDGKKVATLRGANIQDEFRRMVEAYVEQRFGVGGTAAAGASPADAAE

Sequences:

>Translated_448_residues
MPMTTLQSTPATQVPPATTAVVPAELLEAGPAARRPTVGVPVGRVMVGGGAPVVVQSMTNTDTADIEGTVRQVAALARAG
SEIVRITVDRNEAAAAVPHIKEKLLAQGIDVPLVGDFHYIGHKLLADYPACAEALDKYRINPGNVGFGDKKDRQFAAIVE
TAIRHDKPVRIGANWGSLDGELLTRLMDENALLPQPAEARAVTREALVRSALLSAGLAEEIGLPRNRIILSAKVSAVQDL
IAVYGELSRRADYALHLGLTEAGMGSKGIVASTAAMGVVLQAGIGDTIRVSLTPEPNGDRTREVQVAQEILQTMGLRTFV
PLVAACPGCGRTTSTVFQELAQSVQEMIRTRMPVWKAKFPGVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPV
FIDGKKVATLRGANIQDEFRRMVEAYVEQRFGVGGTAAAGASPADAAE
>Mature_447_residues
PMTTLQSTPATQVPPATTAVVPAELLEAGPAARRPTVGVPVGRVMVGGGAPVVVQSMTNTDTADIEGTVRQVAALARAGS
EIVRITVDRNEAAAAVPHIKEKLLAQGIDVPLVGDFHYIGHKLLADYPACAEALDKYRINPGNVGFGDKKDRQFAAIVET
AIRHDKPVRIGANWGSLDGELLTRLMDENALLPQPAEARAVTREALVRSALLSAGLAEEIGLPRNRIILSAKVSAVQDLI
AVYGELSRRADYALHLGLTEAGMGSKGIVASTAAMGVVLQAGIGDTIRVSLTPEPNGDRTREVQVAQEILQTMGLRTFVP
LVAACPGCGRTTSTVFQELAQSVQEMIRTRMPVWKAKFPGVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVF
IDGKKVATLRGANIQDEFRRMVEAYVEQRFGVGGTAAAGASPADAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=405, Percent_Identity=33.0864197530864, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 47023; Mature: 46891

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTTLQSTPATQVPPATTAVVPAELLEAGPAARRPTVGVPVGRVMVGGGAPVVVQSMTN
CCCCCCCCCCCCCCCCCCHHHCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEECCC
TDTADIEGTVRQVAALARAGSEIVRITVDRNEAAAAVPHIKEKLLAQGIDVPLVGDFHYI
CCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCHHHH
GHKLLADYPACAEALDKYRINPGNVGFGDKKDRQFAAIVETAIRHDKPVRIGANWGSLDG
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCH
ELLTRLMDENALLPQPAEARAVTREALVRSALLSAGLAEEIGLPRNRIILSAKVSAVQDL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEHHHHHHHHH
IAVYGELSRRADYALHLGLTEAGMGSKGIVASTAAMGVVLQAGIGDTIRVSLTPEPNGDR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
TREVQVAQEILQTMGLRTFVPLVAACPGCGRTTSTVFQELAQSVQEMIRTRMPVWKAKFP
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFIDGKKVATLRGANIQDEFR
CCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHH
RMVEAYVEQRFGVGGTAAAGASPADAAE
HHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMTTLQSTPATQVPPATTAVVPAELLEAGPAARRPTVGVPVGRVMVGGGAPVVVQSMTN
CCCCCCCCCCCCCCCCCHHHCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEECCC
TDTADIEGTVRQVAALARAGSEIVRITVDRNEAAAAVPHIKEKLLAQGIDVPLVGDFHYI
CCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCHHHH
GHKLLADYPACAEALDKYRINPGNVGFGDKKDRQFAAIVETAIRHDKPVRIGANWGSLDG
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCH
ELLTRLMDENALLPQPAEARAVTREALVRSALLSAGLAEEIGLPRNRIILSAKVSAVQDL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEHHHHHHHHH
IAVYGELSRRADYALHLGLTEAGMGSKGIVASTAAMGVVLQAGIGDTIRVSLTPEPNGDR
HHHHHHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
TREVQVAQEILQTMGLRTFVPLVAACPGCGRTTSTVFQELAQSVQEMIRTRMPVWKAKFP
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GVETLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFIDGKKVATLRGANIQDEFR
CCCEEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHH
RMVEAYVEQRFGVGGTAAAGASPADAAE
HHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA