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The map label for this gene is insK [C]

Identifier: 154245013

GI number: 154245013

Start: 1219283

End: 1220155

Strand: Reverse

Name: insK [C]

Synonym: Xaut_1064

Alternate gene names: 154245013

Gene position: 1220155-1219283 (Counterclockwise)

Preceding gene: 154245014

Following gene: 154245010

Centisome position: 22.98

GC content: 66.32

Gene sequence:

>873_bases
ATGAGCGAACGGCGGGCGTGTAAAGCCACCGGCTTCTGCCGCATGACCATGAGATACAGAGCCACGCGCGGGAATGACGC
CTCGCTGCGTGAGCGCATGAAGGCCATCGCCCAGGAGCGACGGCGGTTTGGCTACCGCCGTCTCCACGTCCTGCTCAGGC
GGGAGGGCTTCCGGGTCAACCACAAGCGGCTGTTCCGGCTCTACCGTGAGGAACGGCTGATGGTCCGCCGGCGAGGCGGT
CGCAAGCGGGCGATCGGGACACGGGCGCCGATGATGATCCCGATGGGACCGAACGAGCGCTGGTCACTCGACTTCGTCGC
CGACCAGATGACCGATGGCCGCCGCTTCCGGATGCTGGCCATCGTGGACGACTGCACCCGCGAGTGCCTGGCGCTGGTGG
CGGACACCTCGCTGTCCGGGGTCAGGGTCGCTCGCGAACTCGACAGGCTTCTGGCAGAGCGCGGCCGGCCCAAGATGATC
GTCAGCGACAATGGCAGCGAGTTCACCTCCAATGCCATTCTGGCCTGGACCGACGCGGCCCGGGTCGAGTGGCACTACAC
CGCGCCCGGCAAGCCGATGCAGAATGGCTTCATCGAGAGCTTCAACGGCCGCCTTCGGGACGAGCTGTTGAACGAGACGC
TGTTCTCCAGCCTGTCCCAGGCCAGGGCCGCCCTCGCCCGATGGCAGGTCGATTACAACACCGACCGACCGCACTCGAAG
CTCGAATGGCAGACGCCAAGCGCCTTCGCCTCAACGTTCCACCCGCGGCGGGATCAGACGCTCCACAACATGAGCGGCTC
CGCGTCTGCCCCCGCCGCCCCGCACACCCGAAAGGGCAAATCCAACCGCCAGAACGAACTCAAAGCTGGATAG

Upstream 100 bases:

>100_bases
CCATGCTGGACAACGTCGCGCTGAAGGATCTCCTGGGAAAGAAGTGGTGACGCCCGCTGCCGAGCGCAGAGCTGTCGCCC
ATCTCGTGGAGCATCACGGG

Downstream 100 bases:

>100_bases
AAGTTGGGGGCAACGTCACACGGCCACTGGCTGGCAATGGGCCGCCCAGGCCGTCATCAGCTCGCGGCGCTTCTCCAAGG
CATCACCCCGGCGATAGGCC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSERRACKATGFCRMTMRYRATRGNDASLRERMKAIAQERRRFGYRRLHVLLRREGFRVNHKRLFRLYREERLMVRRRGG
RKRAIGTRAPMMIPMGPNERWSLDFVADQMTDGRRFRMLAIVDDCTRECLALVADTSLSGVRVARELDRLLAERGRPKMI
VSDNGSEFTSNAILAWTDAARVEWHYTAPGKPMQNGFIESFNGRLRDELLNETLFSSLSQARAALARWQVDYNTDRPHSK
LEWQTPSAFASTFHPRRDQTLHNMSGSASAPAAPHTRKGKSNRQNELKAG

Sequences:

>Translated_290_residues
MSERRACKATGFCRMTMRYRATRGNDASLRERMKAIAQERRRFGYRRLHVLLRREGFRVNHKRLFRLYREERLMVRRRGG
RKRAIGTRAPMMIPMGPNERWSLDFVADQMTDGRRFRMLAIVDDCTRECLALVADTSLSGVRVARELDRLLAERGRPKMI
VSDNGSEFTSNAILAWTDAARVEWHYTAPGKPMQNGFIESFNGRLRDELLNETLFSSLSQARAALARWQVDYNTDRPHSK
LEWQTPSAFASTFHPRRDQTLHNMSGSASAPAAPHTRKGKSNRQNELKAG
>Mature_289_residues
SERRACKATGFCRMTMRYRATRGNDASLRERMKAIAQERRRFGYRRLHVLLRREGFRVNHKRLFRLYREERLMVRRRGGR
KRAIGTRAPMMIPMGPNERWSLDFVADQMTDGRRFRMLAIVDDCTRECLALVADTSLSGVRVARELDRLLAERGRPKMIV
SDNGSEFTSNAILAWTDAARVEWHYTAPGKPMQNGFIESFNGRLRDELLNETLFSSLSQARAALARWQVDYNTDRPHSKL
EWQTPSAFASTFHPRRDQTLHNMSGSASAPAAPHTRKGKSNRQNELKAG

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=247, Percent_Identity=25.5060728744939, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790724, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789424, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789224, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788305, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787669, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786558, Length=245, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786462, Length=180, Percent_Identity=27.2222222222222, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33488; Mature: 33357

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERRACKATGFCRMTMRYRATRGNDASLRERMKAIAQERRRFGYRRLHVLLRREGFRVN
CCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKRLFRLYREERLMVRRRGGRKRAIGTRAPMMIPMGPNERWSLDFVADQMTDGRRFRMLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHCCCCCEEEEEH
IVDDCTRECLALVADTSLSGVRVARELDRLLAERGRPKMIVSDNGSEFTSNAILAWTDAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCEEEEEECCC
RVEWHYTAPGKPMQNGFIESFNGRLRDELLNETLFSSLSQARAALARWQVDYNTDRPHSK
EEEEEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
LEWQTPSAFASTFHPRRDQTLHNMSGSASAPAAPHTRKGKSNRQNELKAG
CCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SERRACKATGFCRMTMRYRATRGNDASLRERMKAIAQERRRFGYRRLHVLLRREGFRVN
CCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKRLFRLYREERLMVRRRGGRKRAIGTRAPMMIPMGPNERWSLDFVADQMTDGRRFRMLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHCCCCCEEEEEH
IVDDCTRECLALVADTSLSGVRVARELDRLLAERGRPKMIVSDNGSEFTSNAILAWTDAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCEEEEEECCC
RVEWHYTAPGKPMQNGFIESFNGRLRDELLNETLFSSLSQARAALARWQVDYNTDRPHSK
EEEEEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
LEWQTPSAFASTFHPRRDQTLHNMSGSASAPAAPHTRKGKSNRQNELKAG
CCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]