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The map label for this gene is yafC [H]

Identifier: 154245010

GI number: 154245010

Start: 1216338

End: 1217243

Strand: Reverse

Name: yafC [H]

Synonym: Xaut_1061

Alternate gene names: 154245010

Gene position: 1217243-1216338 (Counterclockwise)

Preceding gene: 154245013

Following gene: 154245007

Centisome position: 22.93

GC content: 66.11

Gene sequence:

>906_bases
ATGGACAACCGCGCCGGCGAGATGATGGTGTTCGTTCGCGTGGTGGAAGCGGGAAGCTTTTCCGAAGCGGCCCGGCTGAT
GCTGATGACACCCTCCACGGTATCCAAGCTGATCGCCCGCCTGGAAGCGCGGCTGGGGGTGCGCCTGATCGAGCGATCGA
CCCGCCGGCTGGCGCTGACCGGAGAGGGCCAGTTCTATTACGAGCGCAGCCAAGCCCTGCTCGGCCAGCTCGACGAAACC
GAGCAACAGATCGCCCAGGGCGGCGCCGAGGCCGAGGGCGTTGTGCGCGTAACCTCATCGGTGAGCTTCGGCTCCGCGGC
CCTGGAACCGGTCCTGCCGGCCTTCTTCGAGGCCTATCCAAAGGTGATCGTCGATCTCTCGCTCTCGGACGAGGTGGTCG
ATCTCTATCTCGATCGCACCGATGTGGCGATCCGGGTGGGCAAGCTCGTGGATTCCAACCTGATGGCGCGCAAGATCGGC
GAGACGCGGCGGCGTATCGTCGCCTCCCCCGCCTATCTCGCGCGCCACGGCACGCCCGCTACGCCGGAAGATCTCGTCCA
CCACAATTGCCTCGGCTTCAATTTCCGCCGCGCGATGCCGGTCTGGCCCATGCGCGAAGGAGGACGGATCGTCGAGCGCA
TGTTGAGCGGGTCGCTGCTGGTCAATAACGGCGACACGCTTCGCCGTATGGCGATTGCCGGCGTCGGCATCATCAGGCTC
GCCGACTACCATCTGCGCGAGCCCATCGCACGGGGCGAACTGGTGGAGCTTCTGGCCGACACCGACATCGGCGTGACGGA
CGATGTCCATGCCCTGTTCCGCGGCGCGCAGTTCCTGCCCGCCCGCGTCCGGGCCTTCCTGGATTTTGCCGTGCCGCGCA
TGCAGACGTTTTTGGCGCTCGGCTAG

Upstream 100 bases:

>100_bases
CTTTTCACTGGATTTGGGAGATGGTTTCACAGATCGCGGGACGGCCGTCCGATGGCGTGTTAGGTCTCGGCCCTCGGTCA
AGCCAGATCAAAGGTCCGCC

Downstream 100 bases:

>100_bases
GCCACTCACGCGACACGCTCCCCTGCGCACGCCGCCACCGCGTCGGCGGCGGCTGCCTGTGGACGCGGTGGCGGGGACGG
GATCGCAGGATCAGCCTGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDNRAGEMMVFVRVVEAGSFSEAARLMLMTPSTVSKLIARLEARLGVRLIERSTRRLALTGEGQFYYERSQALLGQLDET
EQQIAQGGAEAEGVVRVTSSVSFGSAALEPVLPAFFEAYPKVIVDLSLSDEVVDLYLDRTDVAIRVGKLVDSNLMARKIG
ETRRRIVASPAYLARHGTPATPEDLVHHNCLGFNFRRAMPVWPMREGGRIVERMLSGSLLVNNGDTLRRMAIAGVGIIRL
ADYHLREPIARGELVELLADTDIGVTDDVHALFRGAQFLPARVRAFLDFAVPRMQTFLALG

Sequences:

>Translated_301_residues
MDNRAGEMMVFVRVVEAGSFSEAARLMLMTPSTVSKLIARLEARLGVRLIERSTRRLALTGEGQFYYERSQALLGQLDET
EQQIAQGGAEAEGVVRVTSSVSFGSAALEPVLPAFFEAYPKVIVDLSLSDEVVDLYLDRTDVAIRVGKLVDSNLMARKIG
ETRRRIVASPAYLARHGTPATPEDLVHHNCLGFNFRRAMPVWPMREGGRIVERMLSGSLLVNNGDTLRRMAIAGVGIIRL
ADYHLREPIARGELVELLADTDIGVTDDVHALFRGAQFLPARVRAFLDFAVPRMQTFLALG
>Mature_301_residues
MDNRAGEMMVFVRVVEAGSFSEAARLMLMTPSTVSKLIARLEARLGVRLIERSTRRLALTGEGQFYYERSQALLGQLDET
EQQIAQGGAEAEGVVRVTSSVSFGSAALEPVLPAFFEAYPKVIVDLSLSDEVVDLYLDRTDVAIRVGKLVDSNLMARKIG
ETRRRIVASPAYLARHGTPATPEDLVHHNCLGFNFRRAMPVWPMREGGRIVERMLSGSLLVNNGDTLRRMAIAGVGIIRL
ADYHLREPIARGELVELLADTDIGVTDDVHALFRGAQFLPARVRAFLDFAVPRMQTFLALG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=38.047138047138, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=28.135593220339, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789639, Length=285, Percent_Identity=31.2280701754386, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693193, Length=267, Percent_Identity=31.8352059925094, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87081978, Length=258, Percent_Identity=32.5581395348837, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=27.34375, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=25.5244755244755, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=248, Percent_Identity=30.6451612903226, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788481, Length=304, Percent_Identity=26.9736842105263, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=195, Percent_Identity=29.7435897435897, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=23.3108108108108, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=29.0196078431373, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786448, Length=131, Percent_Identity=33.587786259542, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33211; Mature: 33211

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRAGEMMVFVRVVEAGSFSEAARLMLMTPSTVSKLIARLEARLGVRLIERSTRRLALT
CCCCHHHEEEEEEECCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GEGQFYYERSQALLGQLDETEQQIAQGGAEAEGVVRVTSSVSFGSAALEPVLPAFFEAYP
CCCCEEEHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KVIVDLSLSDEVVDLYLDRTDVAIRVGKLVDSNLMARKIGETRRRIVASPAYLARHGTPA
HHEEEECCCHHHHHHHCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
TPEDLVHHNCLGFNFRRAMPVWPMREGGRIVERMLSGSLLVNNGDTLRRMAIAGVGIIRL
CHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHCCHHHHH
ADYHLREPIARGELVELLADTDIGVTDDVHALFRGAQFLPARVRAFLDFAVPRMQTFLAL
HHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MDNRAGEMMVFVRVVEAGSFSEAARLMLMTPSTVSKLIARLEARLGVRLIERSTRRLALT
CCCCHHHEEEEEEECCCCCHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GEGQFYYERSQALLGQLDETEQQIAQGGAEAEGVVRVTSSVSFGSAALEPVLPAFFEAYP
CCCCEEEHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KVIVDLSLSDEVVDLYLDRTDVAIRVGKLVDSNLMARKIGETRRRIVASPAYLARHGTPA
HHEEEECCCHHHHHHHCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
TPEDLVHHNCLGFNFRRAMPVWPMREGGRIVERMLSGSLLVNNGDTLRRMAIAGVGIIRL
CHHHHHHCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHCCHHHHH
ADYHLREPIARGELVELLADTDIGVTDDVHALFRGAQFLPARVRAFLDFAVPRMQTFLAL
HHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]