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The map label for this gene is potB [H]

Identifier: 154244808

GI number: 154244808

Start: 956912

End: 957838

Strand: Direct

Name: potB [H]

Synonym: Xaut_0858

Alternate gene names: 154244808

Gene position: 956912-957838 (Clockwise)

Preceding gene: 154244807

Following gene: 154244809

Centisome position: 18.02

GC content: 66.99

Gene sequence:

>927_bases
ATGTCCACGGCAACGACCAGCCCTGGCGGTGCCAGCGCGCCGCCCCTCAAGCTTCCCGCGCCGGGTGCGCGGGAGCGCCG
GCGCCTGTCGCCGGCCTTCGGCCTCGCCGCGCCGGGCACCCTGTTCGTGGTGCTGGCGCTGATGGGGCCGCTCGCCCTCA
TGTTCCGCTATTCGCTGAACCGGTTCGTGCCGGGGCAGATGATGGTGGAGGCGCTCACCCTCGCGAACTACCAGAAATTC
TTCGCCGACAGCTACTACCAGGAGGTGCTGGCGACCACCCTGTGGGTGTCCGGCCTCTCCACACTCCTGTGCCTCCTTGC
CGGCTTTCCGGTGGCGTACTTTCTGGTGCGGCAGGCCAGCGACAAATGGAAGGGGCGGTTGCTCCTCCTCGTCATCCTGC
CGCTGCTCATGGGCAATGCGGTGCGCACCGCAGCGTGGATGGTGATTTTGGGTGACAAGGGCCTGTTCAATGCCTTCGTC
GGCACGCTCGGCCTGTCGCCGGTGAAGCTCATGTACACGCCCACCGCCGTGGTCATCGGCCTGGTCTCGGTGTTGCTGCC
GTTCATGATCGTGACGCTGCAGAGCGTTATCGAAGGCATTGACGCCAATCTGGAAGCCGCCGCCTCCAGCCTTGGCGCCT
CCCACCTCACCACCCTGTGGCGGGTGGTGCTGCCCTTGGCGCTGCCGGGCATCCTCGCCGGCACCATGCTGTGCTTCATC
CTCTCCATGAACGCCTATGCGACGCCGGTGCTCATCGGCGGGCCGAGCTTCCAGATGATGGCGCCCGCCGTCTACCAGCA
GGTGGCGAAAGCCATGAACTGGCCCTTCGGCGCGGCGCTCGCCTTCGTGCTCATGGCGGTGACCCTGGTGCTGACCACCA
CCGCCAACGTCCTGGTGCAGCGGCGCTATCGCCGCTGGAGCGAGTGA

Upstream 100 bases:

>100_bases
TCCCCGATTATGCCCACGTGGCCAAGAATACGGCGGAATGGCTCGAGTGGTGGAACAAGAACATCGCCCGCTGAGCGCGG
TCGGCATGTCGGGTTCGGCC

Downstream 100 bases:

>100_bases
GTGCCGCCATGGGAATTCTCGCCATCAACCGCATCTATGCCGCGCTCTCGGCGCTGGTGCTTGCCTTCGTGCTGCTGCCG
CTGGTGGCCATCGTGTGGGT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSTATTSPGGASAPPLKLPAPGARERRRLSPAFGLAAPGTLFVVLALMGPLALMFRYSLNRFVPGQMMVEALTLANYQKF
FADSYYQEVLATTLWVSGLSTLLCLLAGFPVAYFLVRQASDKWKGRLLLLVILPLLMGNAVRTAAWMVILGDKGLFNAFV
GTLGLSPVKLMYTPTAVVIGLVSVLLPFMIVTLQSVIEGIDANLEAAASSLGASHLTTLWRVVLPLALPGILAGTMLCFI
LSMNAYATPVLIGGPSFQMMAPAVYQQVAKAMNWPFGAALAFVLMAVTLVLTTTANVLVQRRYRRWSE

Sequences:

>Translated_308_residues
MSTATTSPGGASAPPLKLPAPGARERRRLSPAFGLAAPGTLFVVLALMGPLALMFRYSLNRFVPGQMMVEALTLANYQKF
FADSYYQEVLATTLWVSGLSTLLCLLAGFPVAYFLVRQASDKWKGRLLLLVILPLLMGNAVRTAAWMVILGDKGLFNAFV
GTLGLSPVKLMYTPTAVVIGLVSVLLPFMIVTLQSVIEGIDANLEAAASSLGASHLTTLWRVVLPLALPGILAGTMLCFI
LSMNAYATPVLIGGPSFQMMAPAVYQQVAKAMNWPFGAALAFVLMAVTLVLTTTANVLVQRRYRRWSE
>Mature_307_residues
STATTSPGGASAPPLKLPAPGARERRRLSPAFGLAAPGTLFVVLALMGPLALMFRYSLNRFVPGQMMVEALTLANYQKFF
ADSYYQEVLATTLWVSGLSTLLCLLAGFPVAYFLVRQASDKWKGRLLLLVILPLLMGNAVRTAAWMVILGDKGLFNAFVG
TLGLSPVKLMYTPTAVVIGLVSVLLPFMIVTLQSVIEGIDANLEAAASSLGASHLTTLWRVVLPLALPGILAGTMLCFIL
SMNAYATPVLIGGPSFQMMAPAVYQQVAKAMNWPFGAALAFVLMAVTLVLTTTANVLVQRRYRRWSE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=235, Percent_Identity=36.5957446808511, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=34.468085106383, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787713, Length=240, Percent_Identity=30.4166666666667, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787368, Length=123, Percent_Identity=32.520325203252, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33074; Mature: 32943

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATTSPGGASAPPLKLPAPGARERRRLSPAFGLAAPGTLFVVLALMGPLALMFRYSLN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFVPGQMMVEALTLANYQKFFADSYYQEVLATTLWVSGLSTLLCLLAGFPVAYFLVRQAS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKWKGRLLLLVILPLLMGNAVRTAAWMVILGDKGLFNAFVGTLGLSPVKLMYTPTAVVIG
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHCCCCCEEEECHHHHHHH
LVSVLLPFMIVTLQSVIEGIDANLEAAASSLGASHLTTLWRVVLPLALPGILAGTMLCFI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSMNAYATPVLIGGPSFQMMAPAVYQQVAKAMNWPFGAALAFVLMAVTLVLTTTANVLVQ
HHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRYRRWSE
HHHHHCCC
>Mature Secondary Structure 
STATTSPGGASAPPLKLPAPGARERRRLSPAFGLAAPGTLFVVLALMGPLALMFRYSLN
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RFVPGQMMVEALTLANYQKFFADSYYQEVLATTLWVSGLSTLLCLLAGFPVAYFLVRQAS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKWKGRLLLLVILPLLMGNAVRTAAWMVILGDKGLFNAFVGTLGLSPVKLMYTPTAVVIG
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHCCCCCEEEECHHHHHHH
LVSVLLPFMIVTLQSVIEGIDANLEAAASSLGASHLTTLWRVVLPLALPGILAGTMLCFI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSMNAYATPVLIGGPSFQMMAPAVYQQVAKAMNWPFGAALAFVLMAVTLVLTTTANVLVQ
HHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRYRRWSE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]