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The map label for this gene is fdoH [H]

Identifier: 154244612

GI number: 154244612

Start: 743007

End: 743978

Strand: Reverse

Name: fdoH [H]

Synonym: Xaut_0661

Alternate gene names: 154244612

Gene position: 743978-743007 (Counterclockwise)

Preceding gene: 154244613

Following gene: 154244611

Centisome position: 14.01

GC content: 65.43

Gene sequence:

>972_bases
ATGTTTCCTCCGATCCCCAACCCTCCGGTCACCCCCACCCCGGCGAAGTTCGGTGACAGCGACCTCGTCCGGCGCTCGGC
CTCCAACCTGCGCGTGCCGGTGGAGTTCAAGGAGCCGGTGGCCAAGCTCATCGACGTGTCCAAGTGCATCGGCTGCAAGG
CCTGCCAGGCGGCCTGCCTTGAATGGAACCAGCTCCACGAGGAGATCGGCACCTTCAACGGCAGCTACACCAACCCGCCG
GACCTGACGCCGAACTCGCTGACCCTGATGCGCTTCACCGAGTGGGTGAACCCCGAGACGGACAATCTCGAATGGCTCAT
CCGCAAGGACGGCTGCATGCATTGCGCCGATCCGGGCTGCCTGAAGGCCTGCCCGGCGCCCGGCGCCATCGTGCAGTATT
CCAACGGCATCGTGGACTTCGACCACGACAAGTGCATCGGCTGCGGCTATTGCGTGAAGGGCTGCCCCTTCAACATTCCC
CGCATCTCGAAGGTCGACAACAAGGCCTACAAGTGCACCTTGTGCTCCGACCGCGTGGCCGTGGGCCAGGGTCCGGCCTG
CCAGAAGGCGTGCCCCACCCAGGCCATCGTGTTCGGCACCAAGACACAGATGAAGGAATGGGCCGAAGGGCGCATCAAGG
ACCTGAAGTCCCGCGGCTACAAGAATGCGGGGCTCTACGATCCGCCCGGCGTCGGCGGCACCCACGTGATGTACGTGCTG
CAGCACGCCGACCAGCCGTCCATCTATGCGGGCCTGCCGGATAATCCGCGCATCAGCCGCATCGTCGAGGCGTGGAAGGG
TGTCACCAAATATGTGGGCCTGGGGGTGATGGGCTTTGCCGCCATCGCCGGCGCCCTGCACTATCTGGTGGCGGGCGCCA
ACAAGGTGACGGCGGAAGACGAGGCCAACGCCGAGAAGCTCGCGCAAGGCCAGCTTCCCGGCGGCGAACCCGCCCATGGA
GGCCGCTCATGA

Upstream 100 bases:

>100_bases
TCGTGGGTGACGCGAACATCGAGACGCCGGAGTTCAAGGCGTTCCTCGTCAACATCGAGCCCTCCACCGGGCCGGTGGCG
TAAGGAGAAGGAGAACCAGC

Downstream 100 bases:

>100_bases
GCCAGCCTTATGACGTTGAATCCGGCGACGCGGTCCATCCCGGCCACCCGGTGCAGGTGGACCGCTACACGGCGGGCGCC
CGCATCAACCACTGGATCAC

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFPPIPNPPVTPTPAKFGDSDLVRRSASNLRVPVEFKEPVAKLIDVSKCIGCKACQAACLEWNQLHEEIGTFNGSYTNPP
DLTPNSLTLMRFTEWVNPETDNLEWLIRKDGCMHCADPGCLKACPAPGAIVQYSNGIVDFDHDKCIGCGYCVKGCPFNIP
RISKVDNKAYKCTLCSDRVAVGQGPACQKACPTQAIVFGTKTQMKEWAEGRIKDLKSRGYKNAGLYDPPGVGGTHVMYVL
QHADQPSIYAGLPDNPRISRIVEAWKGVTKYVGLGVMGFAAIAGALHYLVAGANKVTAEDEANAEKLAQGQLPGGEPAHG
GRS

Sequences:

>Translated_323_residues
MFPPIPNPPVTPTPAKFGDSDLVRRSASNLRVPVEFKEPVAKLIDVSKCIGCKACQAACLEWNQLHEEIGTFNGSYTNPP
DLTPNSLTLMRFTEWVNPETDNLEWLIRKDGCMHCADPGCLKACPAPGAIVQYSNGIVDFDHDKCIGCGYCVKGCPFNIP
RISKVDNKAYKCTLCSDRVAVGQGPACQKACPTQAIVFGTKTQMKEWAEGRIKDLKSRGYKNAGLYDPPGVGGTHVMYVL
QHADQPSIYAGLPDNPRISRIVEAWKGVTKYVGLGVMGFAAIAGALHYLVAGANKVTAEDEANAEKLAQGQLPGGEPAHG
GRS
>Mature_323_residues
MFPPIPNPPVTPTPAKFGDSDLVRRSASNLRVPVEFKEPVAKLIDVSKCIGCKACQAACLEWNQLHEEIGTFNGSYTNPP
DLTPNSLTLMRFTEWVNPETDNLEWLIRKDGCMHCADPGCLKACPAPGAIVQYSNGIVDFDHDKCIGCGYCVKGCPFNIP
RISKVDNKAYKCTLCSDRVAVGQGPACQKACPTQAIVFGTKTQMKEWAEGRIKDLKSRGYKNAGLYDPPGVGGTHVMYVL
QHADQPSIYAGLPDNPRISRIVEAWKGVTKYVGLGVMGFAAIAGALHYLVAGANKVTAEDEANAEKLAQGQLPGGEPAHG
GRS

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=290, Percent_Identity=59.6551724137931, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787749, Length=290, Percent_Identity=58.9655172413793, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1789370, Length=201, Percent_Identity=29.8507462686567, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787122, Length=172, Percent_Identity=33.7209302325581, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787872, Length=172, Percent_Identity=31.9767441860465, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI2367345, Length=156, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI226510944, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082179, Length=174, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789067, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082180, Length=153, Percent_Identity=32.0261437908497, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787478, Length=80, Percent_Identity=35, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=25.7668711656442, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787740, Length=80, Percent_Identity=35, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=27.5641025641026, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 34778; Mature: 34778

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPPIPNPPVTPTPAKFGDSDLVRRSASNLRVPVEFKEPVAKLIDVSKCIGCKACQAACL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCHHHHHHHHH
EWNQLHEEIGTFNGSYTNPPDLTPNSLTLMRFTEWVNPETDNLEWLIRKDGCMHCADPGC
HHHHHHHHHHCCCCCCCCCCCCCCCCEEHEEHHHHCCCCCCCEEEEEECCCCCCCCCCCH
LKACPAPGAIVQYSNGIVDFDHDKCIGCGYCVKGCPFNIPRISKVDNKAYKCTLCSDRVA
HHCCCCCCCEEEECCCEEECCCCCCEECCHHHCCCCCCCCCCHHCCCCEEEEEECCCCEE
VGQGPACQKACPTQAIVFGTKTQMKEWAEGRIKDLKSRGYKNAGLYDPPGVGGTHVMYVL
CCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
QHADQPSIYAGLPDNPRISRIVEAWKGVTKYVGLGVMGFAAIAGALHYLVAGANKVTAED
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EANAEKLAQGQLPGGEPAHGGRS
CCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPPIPNPPVTPTPAKFGDSDLVRRSASNLRVPVEFKEPVAKLIDVSKCIGCKACQAACL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCHHHHHHHHH
EWNQLHEEIGTFNGSYTNPPDLTPNSLTLMRFTEWVNPETDNLEWLIRKDGCMHCADPGC
HHHHHHHHHHCCCCCCCCCCCCCCCCEEHEEHHHHCCCCCCCEEEEEECCCCCCCCCCCH
LKACPAPGAIVQYSNGIVDFDHDKCIGCGYCVKGCPFNIPRISKVDNKAYKCTLCSDRVA
HHCCCCCCCEEEECCCEEECCCCCCEECCHHHCCCCCCCCCCHHCCCCEEEEEECCCCEE
VGQGPACQKACPTQAIVFGTKTQMKEWAEGRIKDLKSRGYKNAGLYDPPGVGGTHVMYVL
CCCCCCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
QHADQPSIYAGLPDNPRISRIVEAWKGVTKYVGLGVMGFAAIAGALHYLVAGANKVTAED
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EANAEKLAQGQLPGGEPAHGGRS
CCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]