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The map label for this gene is fdoI [H]

Identifier: 154244611

GI number: 154244611

Start: 742303

End: 743010

Strand: Reverse

Name: fdoI [H]

Synonym: Xaut_0660

Alternate gene names: 154244611

Gene position: 743010-742303 (Counterclockwise)

Preceding gene: 154244612

Following gene: 154244610

Centisome position: 14.0

GC content: 65.54

Gene sequence:

>708_bases
ATGAGCCAGCCTTATGACGTTGAATCCGGCGACGCGGTCCATCCCGGCCACCCGGTGCAGGTGGACCGCTACACGGCGGG
CGCCCGCATCAACCACTGGATCACGGCCATCAGCCTGATCCTGCTGGCGCTCTCTGGCCTGGCCTTGTTTCACCCCAGCC
TGTTCTTCCTCACCGGCCTGTTCGGCGGGGGGCAGATGACGCGCACCCTCCACCCCTGGATCGGGGTGGTGCTGTTCTTC
TCCTTCTTCGGCCTGTTCTTCCGCTTCTGGAAGGCCAATTTGTGGAAGAGCGAGGACGGCACCTGGCTGCGCCGCATCCG
CGATGTCATCGCCGGCCACGAGGAGAAGCTGCCGGAGCTGGGCAAGTACAATGCTGGCCAGAAGATGGTGTTCTGGGGCA
TGTCGTTCCTGATCGTGGTGCTGATCGCCTCGGGCGTCGTGATCTGGAACGCCTATTTCTCCACCTTCACCAGCATCCCG
CAGCAGCGCGTGGCCCTGCTGGTGCATTCGGTGGCGGCGGTGGCGATCATCTGCGTGTGGATCGTCCACGTCTATGCCGC
CATCTGGGTGAAGGGCACCATCAGCGCCATGACGCGCGGACAGGTGACCGGCGGCTGGGCGTGGCGGCACCACCGCAAAT
GGCTGCGCGAGCTGGTCTCCCGCAAGCCGTCGCAGCCCACCGGCACGCGGGGGACTCCCGCGGAATGA

Upstream 100 bases:

>100_bases
TGGCGGGCGCCAACAAGGTGACGGCGGAAGACGAGGCCAACGCCGAGAAGCTCGCGCAAGGCCAGCTTCCCGGCGGCGAA
CCCGCCCATGGAGGCCGCTC

Downstream 100 bases:

>100_bases
GGGCAGGCGTATACTGTTCGCCCGGCTGCGGTGTCGCGCGGCCGGGGCGGCAGGGTGTTTTAACGCGTTTTCTGCACGCG
AACCGGTATCCACTGCGCTC

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSQPYDVESGDAVHPGHPVQVDRYTAGARINHWITAISLILLALSGLALFHPSLFFLTGLFGGGQMTRTLHPWIGVVLFF
SFFGLFFRFWKANLWKSEDGTWLRRIRDVIAGHEEKLPELGKYNAGQKMVFWGMSFLIVVLIASGVVIWNAYFSTFTSIP
QQRVALLVHSVAAVAIICVWIVHVYAAIWVKGTISAMTRGQVTGGWAWRHHRKWLRELVSRKPSQPTGTRGTPAE

Sequences:

>Translated_235_residues
MSQPYDVESGDAVHPGHPVQVDRYTAGARINHWITAISLILLALSGLALFHPSLFFLTGLFGGGQMTRTLHPWIGVVLFF
SFFGLFFRFWKANLWKSEDGTWLRRIRDVIAGHEEKLPELGKYNAGQKMVFWGMSFLIVVLIASGVVIWNAYFSTFTSIP
QQRVALLVHSVAAVAIICVWIVHVYAAIWVKGTISAMTRGQVTGGWAWRHHRKWLRELVSRKPSQPTGTRGTPAE
>Mature_234_residues
SQPYDVESGDAVHPGHPVQVDRYTAGARINHWITAISLILLALSGLALFHPSLFFLTGLFGGGQMTRTLHPWIGVVLFFS
FFGLFFRFWKANLWKSEDGTWLRRIRDVIAGHEEKLPELGKYNAGQKMVFWGMSFLIVVLIASGVVIWNAYFSTFTSIPQ
QRVALLVHSVAAVAIICVWIVHVYAAIWVKGTISAMTRGQVTGGWAWRHHRKWLRELVSRKPSQPTGTRGTPAE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=208, Percent_Identity=48.5576923076923, Blast_Score=197, Evalue=4e-52,
Organism=Escherichia coli, GI1787750, Length=199, Percent_Identity=40.2010050251256, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 26430; Mature: 26299

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPYDVESGDAVHPGHPVQVDRYTAGARINHWITAISLILLALSGLALFHPSLFFLTGL
CCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGGGQMTRTLHPWIGVVLFFSFFGLFFRFWKANLWKSEDGTWLRRIRDVIAGHEEKLPEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHH
GKYNAGQKMVFWGMSFLIVVLIASGVVIWNAYFSTFTSIPQQRVALLVHSVAAVAIICVW
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
IVHVYAAIWVKGTISAMTRGQVTGGWAWRHHRKWLRELVSRKPSQPTGTRGTPAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQPYDVESGDAVHPGHPVQVDRYTAGARINHWITAISLILLALSGLALFHPSLFFLTGL
CCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGGGQMTRTLHPWIGVVLFFSFFGLFFRFWKANLWKSEDGTWLRRIRDVIAGHEEKLPEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHH
GKYNAGQKMVFWGMSFLIVVLIASGVVIWNAYFSTFTSIPQQRVALLVHSVAAVAIICVW
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
IVHVYAAIWVKGTISAMTRGQVTGGWAWRHHRKWLRELVSRKPSQPTGTRGTPAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]