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The map label for this gene is braC [H]

Identifier: 154244597

GI number: 154244597

Start: 725062

End: 726222

Strand: Reverse

Name: braC [H]

Synonym: Xaut_0645

Alternate gene names: 154244597

Gene position: 726222-725062 (Counterclockwise)

Preceding gene: 154244598

Following gene: 154244596

Centisome position: 13.68

GC content: 65.37

Gene sequence:

>1161_bases
ATGATGATTTCGCGCAGGACGACACTCGCGGCCCTCGCCTTCAGCCTCCTCGCCGGCACGGCGATGGCCGCCGACACCAT
CAAGATCGGCGTCAACGAGCCCCTCACCGGCGCCTTCGCGGCCTCCGGCACCTATGTGGTGAACGGCGCCAAGATCGCCG
CCGACGAGATCAACGCCAAGGGTGGCGTGCTTGGCAAGAAGATCGAGCTCGTCATCGAGGACAACAAGAGCAACCCCACC
GAGGCTGCCTCGGTGGCGGAAAAGCTCATCACCTCCGACAAGGTGCCGGTGCTGATGGGCTCCTGGGGCTCCAGCCTGAC
GCTGGCCACCATGCCGAAGCTGGAGGAATACCAGGTACCCATGCTGGTGGAGACCTCCTCCTCCGGCAAGATTACCACCT
CCGGCAATCCCTTCATCTTCCGCATCTCGCCGCCGTCCTCGGTGGAGGCCGATGCGTTCGAGCCCATCGCCAAGAAGCTC
GGGCTGAAGAAGGTGGACTTCCTCGTCATCAACAATGACTGGGGTCGCGGCGCCGCCGCCGACTTCTCCAAGATGCTGAA
GGCCAACGGCGTGGAGGTCGGCCTGACCGAGACCATGGACCAGTCGGCCCAGGACATGAGCGCCCAGCTCTCCAAGCTGA
AGGCCTCCGATGCCGACACCATCATGGTGACCAGCGCCGTGGACCAGCTGACCCTGCTGTTCAAGCAGATGGCCGCCCTC
GGCCTGAAGAAGCGCGTCATCACCACCGGCGGCTCGCAGAACCCGGACCAGATCATCGCCCAGGCGGGACCTGCGGCCGA
CAACACCATGCACCTCACCACCTTCCTGCCCTGGTGGCCGGAGAAGACCCCGAACCCCGAGGCCACCGCCTATTTCCTCG
GCGAGTGGAAGAAGCGCGGCTTCGAGTTTGCCGGCGCCACCGAGAGCTTCCGCGGCTATGACGGCATCCGCACCGTGGCG
GCTGCCATCGCGGCCGGCGGCGGCACCGATCCCGCCTCCATCCAGAAGGGTTTCTGGAAGGTCGACATGGTGGGCCTCAA
CGGCCCCATCAAGTTCGCCAAGAGCGGCCCGGCCGGCAAGGAGAGCGGCCAGTCGAAGCCCAACGTGTACCTGATCGAGA
TCACCGACGGGAAGGTGAAGCAGGTTCAGCTCGGCGGCTGA

Upstream 100 bases:

>100_bases
AAGCTGCTGACAGCCCTGATTTCCCAAAAGCTCGGCGCCCTGTGGCACCGCATCCGCCCCGCTTCCTGACCCTGACTGCC
CAATGAGACTGGAGACCATC

Downstream 100 bases:

>100_bases
TGCCCGCCCTCGACCGGCGCTTCCCCCGCCTTTGCGCGGGGGCGCCGGGGTGGGGGATCGGGTGCCGTCAACTGCCCTGA
TCGCAAACCTTCCCTCACCC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MMISRRTTLAALAFSLLAGTAMAADTIKIGVNEPLTGAFAASGTYVVNGAKIAADEINAKGGVLGKKIELVIEDNKSNPT
EAASVAEKLITSDKVPVLMGSWGSSLTLATMPKLEEYQVPMLVETSSSGKITTSGNPFIFRISPPSSVEADAFEPIAKKL
GLKKVDFLVINNDWGRGAAADFSKMLKANGVEVGLTETMDQSAQDMSAQLSKLKASDADTIMVTSAVDQLTLLFKQMAAL
GLKKRVITTGGSQNPDQIIAQAGPAADNTMHLTTFLPWWPEKTPNPEATAYFLGEWKKRGFEFAGATESFRGYDGIRTVA
AAIAAGGGTDPASIQKGFWKVDMVGLNGPIKFAKSGPAGKESGQSKPNVYLIEITDGKVKQVQLGG

Sequences:

>Translated_386_residues
MMISRRTTLAALAFSLLAGTAMAADTIKIGVNEPLTGAFAASGTYVVNGAKIAADEINAKGGVLGKKIELVIEDNKSNPT
EAASVAEKLITSDKVPVLMGSWGSSLTLATMPKLEEYQVPMLVETSSSGKITTSGNPFIFRISPPSSVEADAFEPIAKKL
GLKKVDFLVINNDWGRGAAADFSKMLKANGVEVGLTETMDQSAQDMSAQLSKLKASDADTIMVTSAVDQLTLLFKQMAAL
GLKKRVITTGGSQNPDQIIAQAGPAADNTMHLTTFLPWWPEKTPNPEATAYFLGEWKKRGFEFAGATESFRGYDGIRTVA
AAIAAGGGTDPASIQKGFWKVDMVGLNGPIKFAKSGPAGKESGQSKPNVYLIEITDGKVKQVQLGG
>Mature_386_residues
MMISRRTTLAALAFSLLAGTAMAADTIKIGVNEPLTGAFAASGTYVVNGAKIAADEINAKGGVLGKKIELVIEDNKSNPT
EAASVAEKLITSDKVPVLMGSWGSSLTLATMPKLEEYQVPMLVETSSSGKITTSGNPFIFRISPPSSVEADAFEPIAKKL
GLKKVDFLVINNDWGRGAAADFSKMLKANGVEVGLTETMDQSAQDMSAQLSKLKASDADTIMVTSAVDQLTLLFKQMAAL
GLKKRVITTGGSQNPDQIIAQAGPAADNTMHLTTFLPWWPEKTPNPEATAYFLGEWKKRGFEFAGATESFRGYDGIRTVA
AAIAAGGGTDPASIQKGFWKVDMVGLNGPIKFAKSGPAGKESGQSKPNVYLIEITDGKVKQVQLGG

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=255, Percent_Identity=25.8823529411765, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994941, Length=262, Percent_Identity=27.8625954198473, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40670; Mature: 40670

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMISRRTTLAALAFSLLAGTAMAADTIKIGVNEPLTGAFAASGTYVVNGAKIAADEINAK
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCEEEEECCEEEHHHCCCC
GGVLGKKIELVIEDNKSNPTEAASVAEKLITSDKVPVLMGSWGSSLTLATMPKLEEYQVP
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHCCCC
MLVETSSSGKITTSGNPFIFRISPPSSVEADAFEPIAKKLGLKKVDFLVINNDWGRGAAA
EEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
DFSKMLKANGVEVGLTETMDQSAQDMSAQLSKLKASDADTIMVTSAVDQLTLLFKQMAAL
HHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
GLKKRVITTGGSQNPDQIIAQAGPAADNTMHLTTFLPWWPEKTPNPEATAYFLGEWKKRG
CCHHEEEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHCC
FEFAGATESFRGYDGIRTVAAAIAAGGGTDPASIQKGFWKVDMVGLNGPIKFAKSGPAGK
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECCCCEEECCCCCCCC
ESGQSKPNVYLIEITDGKVKQVQLGG
CCCCCCCCEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MMISRRTTLAALAFSLLAGTAMAADTIKIGVNEPLTGAFAASGTYVVNGAKIAADEINAK
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCEEEEECCEEEHHHCCCC
GGVLGKKIELVIEDNKSNPTEAASVAEKLITSDKVPVLMGSWGSSLTLATMPKLEEYQVP
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHCCCC
MLVETSSSGKITTSGNPFIFRISPPSSVEADAFEPIAKKLGLKKVDFLVINNDWGRGAAA
EEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
DFSKMLKANGVEVGLTETMDQSAQDMSAQLSKLKASDADTIMVTSAVDQLTLLFKQMAAL
HHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHH
GLKKRVITTGGSQNPDQIIAQAGPAADNTMHLTTFLPWWPEKTPNPEATAYFLGEWKKRG
CCHHEEEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHCC
FEFAGATESFRGYDGIRTVAAAIAAGGGTDPASIQKGFWKVDMVGLNGPIKFAKSGPAGK
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEEECCCCEEECCCCCCCC
ESGQSKPNVYLIEITDGKVKQVQLGG
CCCCCCCCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]