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The map label for this gene is tusB

Identifier: 153949089

GI number: 153949089

Start: 4401665

End: 4401952

Strand: Reverse

Name: tusB

Synonym: YpsIP31758_3922

Alternate gene names: 153949089

Gene position: 4401952-4401665 (Counterclockwise)

Preceding gene: 153949491

Following gene: 153950162

Centisome position: 93.2

GC content: 43.06

Gene sequence:

>288_bases
ATGCTGTATACCGTCAGTCATTCACCTTATCACTGCGATTTATCTGCGCTGTTGAGATTAGCGACATCTGAGGATGATAT
TTTGTTGCTGCAAGATGGTGTGATAGCGGCCTTGAAAGAGAGTGAAACTCTTAAGCTGTTGTTAAATAACCCCGCTTCTC
TATTTGTATTGGAAGACGATGTTATTGCACGTGGCTTGGTTGGCCAAATTTCAGACAACGCAACACTAATCAGCTATACT
CACTTCGTGGATTTGACCCTAAGACATCAGCAGCAACTGGCATGGTAA

Upstream 100 bases:

>100_bases
TGGATCCTCGATGTAACGGTATTATCCCCGGCAGATTTACGCCGTGAACTGGGCACTTATGATGTTGTGCTGACTTTTTA
ATGAGGTTTGGGTATTCATA

Downstream 100 bases:

>100_bases
TGCGAGTAATGCTGTATATTTCTTGACACCTTAGATAGTCAGCCATAAAATTCTGCGTCCTCGTTCTCTGCCAGTAGTGC
AAACGAGGGGGATTTATCAC

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLYTVSHSPYHCDLSALLRLATSEDDILLLQDGVIAALKESETLKLLLNNPASLFVLEDDVIARGLVGQISDNATLISYT
HFVDLTLRHQQQLAW

Sequences:

>Translated_95_residues
MLYTVSHSPYHCDLSALLRLATSEDDILLLQDGVIAALKESETLKLLLNNPASLFVLEDDVIARGLVGQISDNATLISYT
HFVDLTLRHQQQLAW
>Mature_95_residues
MLYTVSHSPYHCDLSALLRLATSEDDILLLQDGVIAALKESETLKLLLNNPASLFVLEDDVIARGLVGQISDNATLISYT
HFVDLTLRHQQQLAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=50.5263157894737, Blast_Score=97, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_YERP3 (A7FNP2)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402870.1
- ProteinModelPortal:   A7FNP2
- SMR:   A7FNP2
- STRING:   A7FNP2
- GeneID:   5388338
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3922
- eggNOG:   COG2168
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- ProtClustDB:   PRK13510
- BioCyc:   YPSE349747:YPSIP31758_3922-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10577; Mature: 10577

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTVSHSPYHCDLSALLRLATSEDDILLLQDGVIAALKESETLKLLLNNPASLFVLEDD
CEEECCCCCCEECHHHHHHHCCCCCCEEEEECCEEEEECCCCEEEEEECCCEEEEEECCC
VIARGLVGQISDNATLISYTHFVDLTLRHQQQLAW
HHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MLYTVSHSPYHCDLSALLRLATSEDDILLLQDGVIAALKESETLKLLLNNPASLFVLEDD
CEEECCCCCCEECHHHHHHHCCCCCCEEEEECCEEEEECCCCEEEEEECCCEEEEEECCC
VIARGLVGQISDNATLISYTHFVDLTLRHQQQLAW
HHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA