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The map label for this gene is folD [H]

Identifier: 153952430

GI number: 153952430

Start: 884076

End: 884924

Strand: Direct

Name: folD [H]

Synonym: JJD26997_1004

Alternate gene names: 153952430

Gene position: 884076-884924 (Clockwise)

Preceding gene: 153950980

Following gene: 153951289

Centisome position: 47.91

GC content: 32.39

Gene sequence:

>849_bases
ATGACTTTGCTTGATGGTAAAGCTTTAAGCGCAAAAATCAAAGAAGAATTAAAAGAAAAAAATCAATTTTTGAAAAGCAA
AGGGATTGAAAGCTGTTTGGCTGTTATCTTGGTTGGAAATAATCCTGCAAGTCAAACTTATGTAAAATCTAAGGCTAAAG
CCTGTGAAGAATGTGGAATTAAATCTTTAGTTTATCATCTTAATGAAAATACTACACAAAATGAACTTTTAGCATTGATT
AATACCTTAAATCACGATGATAGTGTTCATGGAATTTTGGTTCAACTTCCTTTGCCTGATCATATTTGTAAAGATTTAAT
ACTTGAAAGCATTATAAGTAGTAAAGATGTTGATGGTTTTCATCCTATTAATGTAGGATATTTAAATTTAGGCTTAGAAA
GTGGGTTTTTGCCTTGTACTCCACTTGGAGTTATGAAGCTTTTAAAGGCTTATGAAATTGATTTAAAAGGAAAAGACGCT
GTTATTATAGGTGCTTCAAATATAGTAGGTCGTCCTATGGCTACTATGCTTTTAAATGCTGGAGCTACAGTAAGCGTTTG
TCATATCAAAACCAAAGATTTGAGCCTTTATACTAGGCAAGCTGATTTGATTATAGTAGCAGCAGGTTGTGTGAATTTAC
TTCGATCGGATATGGTAAAAGAAGGAGTGATTGTTGTTGATGTTGGAATCAATCGTCTTGAAAGTGGAAAGATAGTTGGA
GATGTGGATTTTGAAGAGGTATCTAAAAAATCTAGTTATATAACTCCTGTTCCATGTGGAGTAGGTCCTATGACTATAGC
TATGCTTTTAGAAAACACAGTTAAATCTGCTAAAAATAGACTTAATTAG

Upstream 100 bases:

>100_bases
AAACATTAAAACAATTTTCAAAGTTTCACCTTGTTAATGAAGTTTTAATATGATACAATTTTTACTTTAAAAAAGTGATT
AAATTTATAGAAGGAAAATA

Downstream 100 bases:

>100_bases
GAAAAATCTATGGAAATTTTAAAGAAATTATATAAATTTTCGCAGTCTTGGACTGGAACTGTAGTTATTGTTCTTTTGGT
GATTTTTTTCTTTATACAAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGNNPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALI
NTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLKGKDA
VIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVG
DVDFEEVSKKSSYITPVPCGVGPMTIAMLLENTVKSAKNRLN

Sequences:

>Translated_282_residues
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGNNPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALI
NTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLKGKDA
VIIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVG
DVDFEEVSKKSSYITPVPCGVGPMTIAMLLENTVKSAKNRLN
>Mature_281_residues
TLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGNNPASQTYVKSKAKACEECGIKSLVYHLNENTTQNELLALIN
TLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGFHPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLKGKDAV
IIGASNIVGRPMATMLLNAGATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVGD
VDFEEVSKKSSYITPVPCGVGPMTIAMLLENTVKSAKNRLN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.4137931034483, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.8630136986301, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.2702702702703, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1786741, Length=275, Percent_Identity=54.1818181818182, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=261, Percent_Identity=40.6130268199234, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=45.1388888888889, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=41.2162162162162, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=290, Percent_Identity=27.2413793103448, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=44.5945945945946, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=44.5945945945946, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=40.1384083044983, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=40.1384083044983, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=40, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=40, Blast_Score=215, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30382; Mature: 30251

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGNNPASQTYVKSKAKACEECGI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCH
KSLVYHLNENTTQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
HPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLKGKDAVIIGASNIVGRPMATMLLNA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCC
GATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVG
CCEEEEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
DVDFEEVSKKSSYITPVPCGVGPMTIAMLLENTVKSAKNRLN
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLLDGKALSAKIKEELKEKNQFLKSKGIESCLAVILVGNNPASQTYVKSKAKACEECGI
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCH
KSLVYHLNENTTQNELLALINTLNHDDSVHGILVQLPLPDHICKDLILESIISSKDVDGF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCC
HPINVGYLNLGLESGFLPCTPLGVMKLLKAYEIDLKGKDAVIIGASNIVGRPMATMLLNA
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCC
GATVSVCHIKTKDLSLYTRQADLIIVAAGCVNLLRSDMVKEGVIVVDVGINRLESGKIVG
CCEEEEEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
DVDFEEVSKKSSYITPVPCGVGPMTIAMLLENTVKSAKNRLN
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA