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The map label for this gene is trmA [H]

Identifier: 153952276

GI number: 153952276

Start: 1036763

End: 1037836

Strand: Direct

Name: trmA [H]

Synonym: JJD26997_1184

Alternate gene names: 153952276

Gene position: 1036763-1037836 (Clockwise)

Preceding gene: 153952113

Following gene: 153951666

Centisome position: 56.19

GC content: 26.16

Gene sequence:

>1074_bases
ATGAGTCTTGAAAATTTTGGAAATTTTCTCACTTTAGATGAAAAACATTCTTTCATAAAAAAATATTTTAAAGAATTTTA
TACAAAGGATTTTAAACTTTTTACCTCAAAAGATAAGCATTATCGCACAAGAGCTGAGCTTTCATTTTATCATGAAAATG
ATACACTTTTTTATGCGATGTTTGATCCAAAAAGTAAAAAAAAGTACATTATAGAATATTTAGATTTTGCTGATGAAAAA
ATTTGTGCTTTTATGCCAAAACTTTTAGAATACTTACGTCAAGATGATAAATTAAAAGAAAAACTTTTTGGCGTGGAGTT
TTTAACCACAAAACAAGAACTTAGCCTAACTTTACTCTATCATAAAAATATAGAAGATATAAAGTCAAATTTAGAAAATT
TAAGCAATAATCTTCATATCAACCTTATAGCAAGAAGTAAAGGTAAAAAGCTTATTTTTAAAACAGAAAATCTAAGACAA
ACTTTAAATATTCAAGGTAGGGAAATATTTTATGAATTTAACAACGATTGTTTTATTCAGCCTAATACTACTATAAACGA
AAAAATGATTACTTGGGTGTGTGAAATTTTAAGCATTCAAAAAAGAATGGATTTACTTGAACTTTACTGTGGGTATGGAA
ATTTTACTTTAGCTTTAGCACAATTCTTTTTTAAAGTTTTAGCCACTGAAATTTCAAAAAGTAGTATTAATTTTGCTTTA
AAAAATTGCAAGCTTAACAATACAACAAATATCCATTTTGCAAGACTTTCAAGCGAAGAATTAAGCCTAGCTATCAAAAA
AGAAAGGGAATTTTTTCGTTTAAAAGATATTCGATTAGATGATTTTAATTTCTCTCATGTTTTAGTTGATCCACCCCGTG
CAGGACTTGATAAAAGCGTAATTGACTTAATAAAAAAATATGAAAATATTATTTATATTTCTTGTAATCCCATCACTTTA
AAAGAAAATTTAAAAGAATTAAGCCTAACACATAGAGTGGAAAACTTTGCTCTTTTTGATCAATTTGTCAATACTCCACA
TCTTGAATGTGGAGTATTTCTTAGCAAGGTATAA

Upstream 100 bases:

>100_bases
GATATATTCCTGTATCTTTAGGGATTAGCGTTTGGTTTGTCTTGCCTTTAAATTTCATACTTATTGCGCTTTTACTAAGA
CTCATCGGAAAGAAAGTTCC

Downstream 100 bases:

>100_bases
AAAAATAATTCATCAATTTAGCCAGTATTACTATCGCGATACAAAGCACAAATACAAAAGGGTGGATAAAAGCACCGAGC
GGGTTTTTCTTCTTTAATAT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFTSKDKHYRTRAELSFYHENDTLFYAMFDPKSKKKYIIEYLDFADEK
ICAFMPKLLEYLRQDDKLKEKLFGVEFLTTKQELSLTLLYHKNIEDIKSNLENLSNNLHINLIARSKGKKLIFKTENLRQ
TLNIQGREIFYEFNNDCFIQPNTTINEKMITWVCEILSIQKRMDLLELYCGYGNFTLALAQFFFKVLATEISKSSINFAL
KNCKLNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITL
KENLKELSLTHRVENFALFDQFVNTPHLECGVFLSKV

Sequences:

>Translated_357_residues
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFTSKDKHYRTRAELSFYHENDTLFYAMFDPKSKKKYIIEYLDFADEK
ICAFMPKLLEYLRQDDKLKEKLFGVEFLTTKQELSLTLLYHKNIEDIKSNLENLSNNLHINLIARSKGKKLIFKTENLRQ
TLNIQGREIFYEFNNDCFIQPNTTINEKMITWVCEILSIQKRMDLLELYCGYGNFTLALAQFFFKVLATEISKSSINFAL
KNCKLNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITL
KENLKELSLTHRVENFALFDQFVNTPHLECGVFLSKV
>Mature_356_residues
SLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFTSKDKHYRTRAELSFYHENDTLFYAMFDPKSKKKYIIEYLDFADEKI
CAFMPKLLEYLRQDDKLKEKLFGVEFLTTKQELSLTLLYHKNIEDIKSNLENLSNNLHINLIARSKGKKLIFKTENLRQT
LNIQGREIFYEFNNDCFIQPNTTINEKMITWVCEILSIQKRMDLLELYCGYGNFTLALAQFFFKVLATEISKSSINFALK
NCKLNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSVIDLIKKYENIIYISCNPITLK
ENLKELSLTHRVENFALFDQFVNTPHLECGVFLSKV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=351, Percent_Identity=38.1766381766382, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1789148, Length=337, Percent_Identity=27.5964391691395, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787083, Length=234, Percent_Identity=26.4957264957265, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24666579, Length=201, Percent_Identity=28.8557213930348, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42266; Mature: 42135

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFTSKDKHYRTRAELSFYHENDTLFYAM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCHHEEEEEEEEEECCCEEEEEE
FDPKSKKKYIIEYLDFADEKICAFMPKLLEYLRQDDKLKEKLFGVEFLTTKQELSLTLLY
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCHHEEECHHHCEEEEEE
HKNIEDIKSNLENLSNNLHINLIARSKGKKLIFKTENLRQTLNIQGREIFYEFNNDCFIQ
ECCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHCCCCEEEEEEECCEEEEC
PNTTINEKMITWVCEILSIQKRMDLLELYCGYGNFTLALAQFFFKVLATEISKSSINFAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
KNCKLNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSV
EECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCCHHH
IDLIKKYENIIYISCNPITLKENLKELSLTHRVENFALFDQFVNTPHLECGVFLSKV
HHHHHHHCCEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECC
>Mature Secondary Structure 
SLENFGNFLTLDEKHSFIKKYFKEFYTKDFKLFTSKDKHYRTRAELSFYHENDTLFYAM
CCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCHHEEEEEEEEEECCCEEEEEE
FDPKSKKKYIIEYLDFADEKICAFMPKLLEYLRQDDKLKEKLFGVEFLTTKQELSLTLLY
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCHHEEECHHHCEEEEEE
HKNIEDIKSNLENLSNNLHINLIARSKGKKLIFKTENLRQTLNIQGREIFYEFNNDCFIQ
ECCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHCCCCEEEEEEECCEEEEC
PNTTINEKMITWVCEILSIQKRMDLLELYCGYGNFTLALAQFFFKVLATEISKSSINFAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
KNCKLNNTTNIHFARLSSEELSLAIKKEREFFRLKDIRLDDFNFSHVLVDPPRAGLDKSV
EECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCCHHH
IDLIKKYENIIYISCNPITLKENLKELSLTHRVENFALFDQFVNTPHLECGVFLSKV
HHHHHHHCCEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA