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The map label for this gene is nth [H]

Identifier: 153952219

GI number: 153952219

Start: 949538

End: 950164

Strand: Direct

Name: nth [H]

Synonym: JJD26997_1074

Alternate gene names: 153952219

Gene position: 949538-950164 (Clockwise)

Preceding gene: 153952391

Following gene: 153951154

Centisome position: 51.46

GC content: 32.22

Gene sequence:

>627_bases
ATGAAAAGAAATTTAGAAATCAAAGAACTTTTTTTAAAACATTTTGACAAGCCTGTAACAGAGCTTAAATTTTCAAATCT
ATATGAGCTTTTAGTCTGCGTGATGCTTTCAGCCCAATGCACTGATAAAAGAGTTAATCTCATCACTCCTGATCTTTTTA
AGACTTATCCTGATATCAAATCCCTTGCAAATGCAAATTTATCAAGTCTTAAAACCTATATACAAACTTGTTCATTTTTT
AATAATAAAGCACAAAATCTTATAAAAATGGCAAAAGCAGTTTGTGAAAATTTTGACGGAGAAATTCCCCTTGATGAACA
AAATTTAAAATCCTTAGCAGGCGTAGGACAAAAAACCGCTCATGTAGTTCTAATAGAATGGTGCGGAGCAAATTTCATGG
CTGTAGATACTCATGTTTTTAGAGTTTCTCACCGCTTAGGGCTTAGCAAAGCAAAAACTCCAGAGGCTACAGAAGAAGAT
TTAACACGCACATTTAAAGATAATCTTAATTATCTTCATCAAGCTATGGTTCTTTTTGGGCGCTACACCTGTAAAGCTAA
AAAACCTTTATGTAAGGAATGTTTTTTAAATCATCTTTGCAAAAGTAAAGATAAGGAACTTAAATGA

Upstream 100 bases:

>100_bases
ACTAAAGAAAATTTTTTCATTAGGTTTCCTTTTTAATAAAATATATATAAAACCAATTATATCTAAACTTGGTTTAAAGC
TAATTAACTAAAATTAAAAA

Downstream 100 bases:

>100_bases
AAATTTTCATCTTTTTACTCGCTATATCTCTTAATATTTTCGCCTTAGAATCTTACAAACCTAGTGCTGATTTTTCTTCT
TATTTTAACAATATAAATTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKRNLEIKELFLKHFDKPVTELKFSNLYELLVCVMLSAQCTDKRVNLITPDLFKTYPDIKSLANANLSSLKTYIQTCSFF
NNKAQNLIKMAKAVCENFDGEIPLDEQNLKSLAGVGQKTAHVVLIEWCGANFMAVDTHVFRVSHRLGLSKAKTPEATEED
LTRTFKDNLNYLHQAMVLFGRYTCKAKKPLCKECFLNHLCKSKDKELK

Sequences:

>Translated_208_residues
MKRNLEIKELFLKHFDKPVTELKFSNLYELLVCVMLSAQCTDKRVNLITPDLFKTYPDIKSLANANLSSLKTYIQTCSFF
NNKAQNLIKMAKAVCENFDGEIPLDEQNLKSLAGVGQKTAHVVLIEWCGANFMAVDTHVFRVSHRLGLSKAKTPEATEED
LTRTFKDNLNYLHQAMVLFGRYTCKAKKPLCKECFLNHLCKSKDKELK
>Mature_208_residues
MKRNLEIKELFLKHFDKPVTELKFSNLYELLVCVMLSAQCTDKRVNLITPDLFKTYPDIKSLANANLSSLKTYIQTCSFF
NNKAQNLIKMAKAVCENFDGEIPLDEQNLKSLAGVGQKTAHVVLIEWCGANFMAVDTHVFRVSHRLGLSKAKTPEATEED
LTRTFKDNLNYLHQAMVLFGRYTCKAKKPLCKECFLNHLCKSKDKELK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=30.2197802197802, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787920, Length=204, Percent_Identity=39.7058823529412, Blast_Score=151, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=34.2696629213483, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=29.0155440414508, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23786; Mature: 23786

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNLEIKELFLKHFDKPVTELKFSNLYELLVCVMLSAQCTDKRVNLITPDLFKTYPDIK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHH
SLANANLSSLKTYIQTCSFFNNKAQNLIKMAKAVCENFDGEIPLDEQNLKSLAGVGQKTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHH
HVVLIEWCGANFMAVDTHVFRVSHRLGLSKAKTPEATEEDLTRTFKDNLNYLHQAMVLFG
HEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RYTCKAKKPLCKECFLNHLCKSKDKELK
HHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRNLEIKELFLKHFDKPVTELKFSNLYELLVCVMLSAQCTDKRVNLITPDLFKTYPDIK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHH
SLANANLSSLKTYIQTCSFFNNKAQNLIKMAKAVCENFDGEIPLDEQNLKSLAGVGQKTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHH
HVVLIEWCGANFMAVDTHVFRVSHRLGLSKAKTPEATEEDLTRTFKDNLNYLHQAMVLFG
HEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RYTCKAKKPLCKECFLNHLCKSKDKELK
HHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA