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The map label for this gene is acnB [H]

Identifier: 153951795

GI number: 153951795

Start: 1030446

End: 1032992

Strand: Direct

Name: acnB [H]

Synonym: JJD26997_1179

Alternate gene names: 153951795

Gene position: 1030446-1032992 (Clockwise)

Preceding gene: 153951209

Following gene: 153952145

Centisome position: 55.85

GC content: 36.24

Gene sequence:

>2547_bases
ATGTCTTTTATGCAAGAATACCCTAAACTTGTTGAAGAAAGAGCCGCTTTGGGTATACCACCGCTTCCTTTAAATGCAAA
TCAAACTCAAGAACTTTGTAAATTACTTGAAAATGAAAACAATGAAGAACTTGCCAATCTTTTAGAAAATAGAGTAAATC
CTGGTGTTGATGATGCAGCTTTAGTGAAATGTGAATTTTTAGATTCCATACTTAAAGATAAAATCAGTACTCCAAACATA
GATAAAAAAAGAGCTTTAAGAATGCTTGGAACTATGTTAGGTGGATACAATGTTAAAGTTTTAATCGATGCCTTAAAAGA
TGAAAACATAGCCAAAGATGCTGCAGAAGTGTTGAAAAATATTATTTTTGTACACGATAATTTTCATACTATAGCCGAGC
TTAGCAAAAACAATCCACACGCTAAAGAAGTTTTACAAAGCTGGGCTAATGCAGACTGGTTTAACAAAAAAGAAAAATTG
CCACAAGTGATTCAATGTATAGTTTTTAAAGTAGCAGGAGAAACAAATACTGATGATCTAAGCCCTGCAGGAGATGCTTT
TACAAGAAGTGATATTCCTTTGCATGCAAATGCCATGCTAAAAGTAAGACAAACAGGATCCTTAGAAAAAATCAAAGAGC
TTAAAAAAAGCGCTCGCGAAGTGGTTTATGTAGGTGATGTTGTAGGAACAGGTTCAAGTAGAAAATCGGCAATAAATTCT
ATACAATGGCATTTAGGAAAAGAAATAGAAGGGGTGCCAAACAAGCATAGCGGTGGTATAGTAATGGGTTCAACTATAGC
ACCAATTTTCTTTAACACTGCTCAAGATAGCGGGGCTTTGCCTATCATCTGTGATGTAACAAACTTAGAAATGGGCGATG
AGTTTGAAATTCATCCTTATGAGGGAAAAATCATAAAAAATGGCTCTACAATCGCAGAATTTACACTCAGTCCAAACACT
CTCTTAGATGAAGTTAGAGCAGGAGGAAGAATCCCACTTATCATAGGTCGTGGACTTTGTGCCAAGGCTAGAGAATTTTT
AGGAATGGAAAGTGAAAATATCTTTACTAAGCCTGAACAACCTAAATCTTCAAGCGGTGGTTATACCTTAGCTCAAAAAA
TGCTAGGTCGTGCCTGTGGAGTTGAAGGTGTGTGTCCAGGTATGTATATAGAACCTATGACTTTAACTGTAGGCTCTCAA
GATACCACAGGTCCTATGACTAGAGATGAAATTAAAGAACTTGCAAGCCTGGGTTTTAATGCAGACTTTGTGATGCAAAG
CTTTTGCCATACAGCTGCTTATCCAAAAGTAAGCGATTCAAATTTACATAAAACTTTACCCAATTTCATGACAAGTCGTG
GTGGGGTGAGTTTAAAACCAGGTGATGGGGTTATCCACTCTTGGCTTAATCGTTTTGTATTGCCAGATAGTGTGGGAACA
GGCGGAGATTCTCATACACGCTTTCCTATAGGAATTTCTTTCCCTGCAGGAAGCGGACTTGTAGCTTTTGCTGGGGTTAC
TGGTTCTATGCCTTTAAATGTCCCTGAAAGTGTTTTAGTGCGTTTTAGTGGAGAATTACAAGCTGGGGTTACCTTAAGAG
ATTTAGTTAATGCTATACCTTATTATGCGATCAAACAAGGACAGCTTACCGTTGAAAAGAAAAATAAGAAAAATATTTTT
GCAGGAAAAATTTTAGAAATAGAAGGCTTACCAAACTTAAAAGTCGAACAAGCTTTTGAACTCAGCGATGCAAGTGCTGA
AAGATCTGCAGCTGCTTGTAGTGTGGATTTGAGCATAGAAAGTGTAAGCGAATATATCAAATCCAATATCTCTTTAATTG
AAGCTATGATAGAAGCAGGATATGAGAATAAAGCCACCCTTGCTAGAAGAGCTGAAAAAATGAGAGAATGGCTTAAAAAC
CCTACTCTTTTAAGAGCAGATAAAGATGCAAAATACGCTTATATTATAAATATTAATCTTAATGACATTAAAGAGCCAAT
CCTAGCTTGTCCAAATGACCCTGATGATGTGGCGACTTTGAGTGAAATTTTAGCCGATGATAAGCGTCCAAAAAATATAG
ATGAAGTTTTTGTAGGTTCCTGTATGACAAACATAGGACATTATAGAGCTTTAGGAGAAATTTTAAAAGATAAAGGCATA
CTTAAAACCAGACTTTGGGTAGTACCTCCAACAAAAATGGATAAAGCACAACTTACTGATGAAGGTTATTATAGCATCTT
TGGTGCAGCAGGTGCTAGAATAGAAGTTCCTGGTTGTTCTTTATGTATGGGTAATCAAGCTAGAGTAAATGATGGAGCTG
TAGTTTTCTCAACCTCTACAAGAAATTTTGACAACCGCATGGGAATGGGTGCTAAAGTATATCTAGGCAGTGCAGAACTT
GCAGCAGTTTGTGCGATTTTAGGTAAAATTCCAAGCAAGGAAGAATACTTACAAATTGTCAGCGAAAAACTAAGCGATGA
GCATAAGGCAAATATCTATAGATATTTAAATTTTAATGAGATTGAAAATTTCAAACTTGAAAATTAA

Upstream 100 bases:

>100_bases
GTAAAGATAGGTAACACAAAGGTATTTTATAATAAACTTTAAACATTTTTTATCCTTTAAAGAGCTACAATGATAATAAA
AAATATTTAAGGAGTAGAAA

Downstream 100 bases:

>100_bases
TTCAAATTTAATACTTATTTGGATTAAATAACAAAATTAAAATTTTCAAAGGAAAAATATTGAAGTTATTATTCACTTCC
TTTTGTTGTTTTTTAAGTTT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 848; Mature: 847

Protein sequence:

>848_residues
MSFMQEYPKLVEERAALGIPPLPLNANQTQELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKDKISTPNI
DKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKL
PQVIQCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSAREVVYVGDVVGTGSSRKSAINS
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYEGKIIKNGSTIAEFTLSPNT
LLDEVRAGGRIPLIIGRGLCAKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVCPGMYIEPMTLTVGSQ
DTTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDSVGT
GGDSHTRFPIGISFPAGSGLVAFAGVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIF
AGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKN
PTLLRADKDAKYAYIININLNDIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
LKTRLWVVPPTKMDKAQLTDEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAEL
AAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN

Sequences:

>Translated_848_residues
MSFMQEYPKLVEERAALGIPPLPLNANQTQELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKDKISTPNI
DKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKL
PQVIQCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSAREVVYVGDVVGTGSSRKSAINS
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYEGKIIKNGSTIAEFTLSPNT
LLDEVRAGGRIPLIIGRGLCAKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVCPGMYIEPMTLTVGSQ
DTTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDSVGT
GGDSHTRFPIGISFPAGSGLVAFAGVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIF
AGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKN
PTLLRADKDAKYAYIININLNDIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
LKTRLWVVPPTKMDKAQLTDEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAEL
AAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN
>Mature_847_residues
SFMQEYPKLVEERAALGIPPLPLNANQTQELCKLLENENNEELANLLENRVNPGVDDAALVKCEFLDSILKDKISTPNID
KKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKNIIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLP
QVIQCIVFKVAGETNTDDLSPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSAREVVYVGDVVGTGSSRKSAINSI
QWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPYEGKIIKNGSTIAEFTLSPNTL
LDEVRAGGRIPLIIGRGLCAKAREFLGMESENIFTKPEQPKSSSGGYTLAQKMLGRACGVEGVCPGMYIEPMTLTVGSQD
TTGPMTRDEIKELASLGFNADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDSVGTG
GDSHTRFPIGISFPAGSGLVAFAGVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIPYYAIKQGQLTVEKKNKKNIFA
GKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIESVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNP
TLLRADKDAKYAYIININLNDIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGIL
KTRLWVVPPTKMDKAQLTDEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTSTRNFDNRMGMGAKVYLGSAELA
AVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNEIENFKLEN

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=859, Percent_Identity=59.953434225844, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=371, Percent_Identity=26.1455525606469, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323335, Length=389, Percent_Identity=24.6786632390745, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92706; Mature: 92575

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFMQEYPKLVEERAALGIPPLPLNANQTQELCKLLENENNEELANLLENRVNPGVDDAA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
LVKCEFLDSILKDKISTPNIDKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHH
IIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLPQVIQCIVFKVAGETNTDDL
HEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSAREVVYVGDVVGTGSSRKSAINS
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEECEECCCCCHHHHHHH
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPY
HHHHCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEECC
EGKIIKNGSTIAEFTLSPNTLLDEVRAGGRIPLIIGRGLCAKAREFLGMESENIFTKPEQ
CCEEEECCCEEEEEEECCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCC
PKSSSGGYTLAQKMLGRACGVEGVCPGMYIEPMTLTVGSQDTTGPMTRDEIKELASLGFN
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCCCCCCCCHHHHHHHHHCCCC
ADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDSVGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCCCCC
GGDSHTRFPIGISFPAGSGLVAFAGVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIP
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHCC
YYAIKQGQLTVEKKNKKNIFAGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIE
CEEEECCEEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEEECCHH
SVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNPTLLRADKDAKYAYIININL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECH
NDIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKTRLWVVPPTKMDKAQLTDEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTST
EEEEEEEECCCCCCHHHCCCCCCEEEEECCCCEEECCCCCEECCCCCEECCCEEEEEECC
RNFDNRMGMGAKVYLGSAELAAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNE
CCCHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEECCCC
IENFKLEN
CCCCCCCC
>Mature Secondary Structure 
SFMQEYPKLVEERAALGIPPLPLNANQTQELCKLLENENNEELANLLENRVNPGVDDAA
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
LVKCEFLDSILKDKISTPNIDKKRALRMLGTMLGGYNVKVLIDALKDENIAKDAAEVLKN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHHHHHH
IIFVHDNFHTIAELSKNNPHAKEVLQSWANADWFNKKEKLPQVIQCIVFKVAGETNTDDL
HEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SPAGDAFTRSDIPLHANAMLKVRQTGSLEKIKELKKSAREVVYVGDVVGTGSSRKSAINS
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEECEECCCCCHHHHHHH
IQWHLGKEIEGVPNKHSGGIVMGSTIAPIFFNTAQDSGALPIICDVTNLEMGDEFEIHPY
HHHHCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEECC
EGKIIKNGSTIAEFTLSPNTLLDEVRAGGRIPLIIGRGLCAKAREFLGMESENIFTKPEQ
CCEEEECCCEEEEEEECCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCC
PKSSSGGYTLAQKMLGRACGVEGVCPGMYIEPMTLTVGSQDTTGPMTRDEIKELASLGFN
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCCCCCCCCHHHHHHHHHCCCC
ADFVMQSFCHTAAYPKVSDSNLHKTLPNFMTSRGGVSLKPGDGVIHSWLNRFVLPDSVGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCCCCC
GGDSHTRFPIGISFPAGSGLVAFAGVTGSMPLNVPESVLVRFSGELQAGVTLRDLVNAIP
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHCC
YYAIKQGQLTVEKKNKKNIFAGKILEIEGLPNLKVEQAFELSDASAERSAAACSVDLSIE
CEEEECCEEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCHHHCCCEEEEECCHH
SVSEYIKSNISLIEAMIEAGYENKATLARRAEKMREWLKNPTLLRADKDAKYAYIININL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECH
NDIKEPILACPNDPDDVATLSEILADDKRPKNIDEVFVGSCMTNIGHYRALGEILKDKGI
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKTRLWVVPPTKMDKAQLTDEGYYSIFGAAGARIEVPGCSLCMGNQARVNDGAVVFSTST
EEEEEEEECCCCCCHHHCCCCCCEEEEECCCCEEECCCCCEECCCCCEECCCEEEEEECC
RNFDNRMGMGAKVYLGSAELAAVCAILGKIPSKEEYLQIVSEKLSDEHKANIYRYLNFNE
CCCHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEECCCC
IENFKLEN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]