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The map label for this gene is thrB

Identifier: 153936225

GI number: 153936225

Start: 1751387

End: 1752280

Strand: Direct

Name: thrB

Synonym: CLC_1665

Alternate gene names: 153936225

Gene position: 1751387-1752280 (Clockwise)

Preceding gene: 153936037

Following gene: 153936863

Centisome position: 46.57

GC content: 33.0

Gene sequence:

>894_bases
ATGGTAGAAGTTAGAGTGCCTGCTACAAGTGCAAATATAGGACCAGGATTTGATTGCTTAGGTGTAGCAGTAAATATGTA
TAATAAATTCTTTGTAGAAGAAATTGAAGAGGGACTTATCTTTGAAGGTTGTGCGGATAAATTTAAAAATGAGAACAACT
TAATATATGTGGCTATGAAAAAGTGTTTTGATAAAATCGGATACAAACCTACAGGATTAAGGATAAAAATAGAAAGCGAT
ATACCTGTCTCAAGAGGGCTTGGAAGTAGTGCAGCCTGTGTGGTAGGAGGTATAGTTTCTGCCAATGAATTAGCTGGTGG
AGCTTTAAATAAAAAAGAACTTTTAGATTTAGCAGTGGAAGTGGAAGGGCATCCGGACAATGTAAATCCAGCTTTTTGTG
GGGGAATGACTGCTTCCATATCTGATAATAGAGAAGTAATTTATAGTAAAGTGAAGGTGAGTGAGGGTATTAAGTTTTGT
GCACTTATACCAGATTTTACTTTATCTACAGAAAAGGCAAGGGCAGTCCTTCCTAAAAGTATAGATTACAAAGATGGAAT
TTTTAATGTAGGAAGAACCGCACTTATGATATCTGCATTAAATAATGGTGATTTTCATTTAATAAAATATGCTTGCAAGG
ATAAATTACATCAAGACTATAGAGCAAAGCTTATAGAAAATTTTTATTCAATAAAAAAACAGTGTGAAAAACTAAATTCT
TTAGGTGTATTTTTAAGTGGAGCAGGTCCAACAATTATGGTAATGCTTAGAGAAGAGGATAAGGATTTTTCAAAAAATAT
TAAAAGTTTTTTAGAGACTTTAAAGAACAAGTGGGAAGTTAGGGAACTTAAGATAGACAAGCTTGGAACCGTAGTAAATA
ATTGTAAGGTTTAA

Upstream 100 bases:

>100_bases
AGAAGGACTAGAGGAAAAAGAAATTTGTCATAAGGTCACTTGTAAAAAGGATGAAATGAAGAAAGTAATTAAAAATTTTT
TAGGAATATAGGTGATTAAA

Downstream 100 bases:

>100_bases
ATTGAATAAGTTTAAGTAAATTATTAAATTTAAAAAATTAGATAGTTTAGAGTAAGAAATGAAAGGATCTTTAATATATT
GATTACTGTGAATTATTAAT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVEVRVPATSANIGPGFDCLGVAVNMYNKFFVEEIEEGLIFEGCADKFKNENNLIYVAMKKCFDKIGYKPTGLRIKIESD
IPVSRGLGSSAACVVGGIVSANELAGGALNKKELLDLAVEVEGHPDNVNPAFCGGMTASISDNREVIYSKVKVSEGIKFC
ALIPDFTLSTEKARAVLPKSIDYKDGIFNVGRTALMISALNNGDFHLIKYACKDKLHQDYRAKLIENFYSIKKQCEKLNS
LGVFLSGAGPTIMVMLREEDKDFSKNIKSFLETLKNKWEVRELKIDKLGTVVNNCKV

Sequences:

>Translated_297_residues
MVEVRVPATSANIGPGFDCLGVAVNMYNKFFVEEIEEGLIFEGCADKFKNENNLIYVAMKKCFDKIGYKPTGLRIKIESD
IPVSRGLGSSAACVVGGIVSANELAGGALNKKELLDLAVEVEGHPDNVNPAFCGGMTASISDNREVIYSKVKVSEGIKFC
ALIPDFTLSTEKARAVLPKSIDYKDGIFNVGRTALMISALNNGDFHLIKYACKDKLHQDYRAKLIENFYSIKKQCEKLNS
LGVFLSGAGPTIMVMLREEDKDFSKNIKSFLETLKNKWEVRELKIDKLGTVVNNCKV
>Mature_297_residues
MVEVRVPATSANIGPGFDCLGVAVNMYNKFFVEEIEEGLIFEGCADKFKNENNLIYVAMKKCFDKIGYKPTGLRIKIESD
IPVSRGLGSSAACVVGGIVSANELAGGALNKKELLDLAVEVEGHPDNVNPAFCGGMTASISDNREVIYSKVKVSEGIKFC
ALIPDFTLSTEKARAVLPKSIDYKDGIFNVGRTALMISALNNGDFHLIKYACKDKLHQDYRAKLIENFYSIKKQCEKLNS
LGVFLSGAGPTIMVMLREEDKDFSKNIKSFLETLKNKWEVRELKIDKLGTVVNNCKV

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=271, Percent_Identity=31.3653136531365, Blast_Score=117, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321814, Length=350, Percent_Identity=30.5714285714286, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_CLOB1 (A7FUE5)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383978.1
- ProteinModelPortal:   A7FUE5
- SMR:   A7FUE5
- STRING:   A7FUE5
- GeneID:   5395089
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1656
- eggNOG:   COG0083
- HOGENOM:   HBG646290
- OMA:   NNALMST
- ProtClustDB:   PRK01212
- BioCyc:   CBOT441770:CLB_1656-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 32698; Mature: 32698

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVRVPATSANIGPGFDCLGVAVNMYNKFFVEEIEEGLIFEGCADKFKNENNLIYVAMK
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCEEEEEHH
KCFDKIGYKPTGLRIKIESDIPVSRGLGSSAACVVGGIVSANELAGGALNKKELLDLAVE
HHHHHHCCCCCCEEEEECCCCCHHCCCCCCCHHHHHCCCCHHHHCCCCCCHHHHEEEEEE
VEGHPDNVNPAFCGGMTASISDNREVIYSKVKVSEGIKFCALIPDFTLSTEKARAVLPKS
ECCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCC
IDYKDGIFNVGRTALMISALNNGDFHLIKYACKDKLHQDYRAKLIENFYSIKKQCEKLNS
CCCCCCHHHCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLSGAGPTIMVMLREEDKDFSKNIKSFLETLKNKWEVRELKIDKLGTVVNNCKV
CEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHCCCC
>Mature Secondary Structure
MVEVRVPATSANIGPGFDCLGVAVNMYNKFFVEEIEEGLIFEGCADKFKNENNLIYVAMK
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCEEEEEHH
KCFDKIGYKPTGLRIKIESDIPVSRGLGSSAACVVGGIVSANELAGGALNKKELLDLAVE
HHHHHHCCCCCCEEEEECCCCCHHCCCCCCCHHHHHCCCCHHHHCCCCCCHHHHEEEEEE
VEGHPDNVNPAFCGGMTASISDNREVIYSKVKVSEGIKFCALIPDFTLSTEKARAVLPKS
ECCCCCCCCCHHHCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCC
IDYKDGIFNVGRTALMISALNNGDFHLIKYACKDKLHQDYRAKLIENFYSIKKQCEKLNS
CCCCCCHHHCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLSGAGPTIMVMLREEDKDFSKNIKSFLETLKNKWEVRELKIDKLGTVVNNCKV
CEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA