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The map label for this gene is yclM [H]

Identifier: 153936863

GI number: 153936863

Start: 1752407

End: 1753726

Strand: Direct

Name: yclM [H]

Synonym: CLC_1666

Alternate gene names: 153936863

Gene position: 1752407-1753726 (Clockwise)

Preceding gene: 153936225

Following gene: 153936966

Centisome position: 46.6

GC content: 30.91

Gene sequence:

>1320_bases
ATGGGAAAAGTAATCGTAGCTAAGTTTGGAGGAAGTTCACTTTCAGACAGTGAACAATTTAGAAAGGTGAAAAGTATAAT
TTATGATAATGAAAACAGAAGATACATAGTACCTTCAGCGCCAGGTAAAAGATTTAAAAAGGATTATAAAATAACAGATC
TTTTATATTTATGTCATGCCCATAGAGAAAGTGCTATATCCTTTGATGACGTGTTTAAGCATATAGAGGAAAGATATTTA
AATTTAGCAAAGGAATTAAGGGTTAAAGTTGATATAAAAAATAAACTAGAAGAAATAAAAAGGGAGATTGAAGCAGGTGC
CTCAAGAGATTTTGCAGCTAGCAGAGGAGAATATTTAAACGGATTAATTTTGGCAGATTATTTAAATTATGAATTTATAG
ATGCAGCTGAAATTATACATTTCAAAAAATATGGGAGCTTAGATTTAAGAAAAACAGAGAAGGCTATAAAAGAAAAAATT
AAGAATGTTAAAAAGGCAGTTATTCCAGGCTTTTATGGAGCGCTGCCTGATGGAACTATAAAAACTTTTTCAAGAGGTGG
TTCTGATGTAACAGGAGCTATAGTAGCTAGAGCTGTAGAGGCTTCTCTTTATGAGAATTGGACAGATGTCTCAGGTTTTT
TAATGGCAGATCCTAATATTATAAATGATCCAAAACCTATAGAAGTAATAAGCTATAAGGAACTTAGAGAACTTTCTTAT
ATGGGAGCAAAGGTTCTTCATGAAGAGAGTATATTTCCTGTAAGAGATGTGAAAATTCCTATAAACATAAAGAATACTAA
TAGAGCAGAAGATAAAGGTACATTAATTGTAGATGATGATAAAGCTTTAAATTATACTGGATCTATTACAGGCATAGCAG
GTAAAAAAGGATTTACAGTCATAGCTATACACAAGATGCTTATGAATTCAGAATTAGGTTTTTGTAGAAAACTTCTTTCT
ATACTAGAAGAAAATGGGGTAGCCTTTGAAAATATTCCCTCAGGTATAGATTCTGTATCATTAGTAATAGAAGACAGTCA
ACTTGGTAATAAGCTAGATATTATATTAGAAGAAATTAAAAGACAGTGTAATCCAGATTCTTTAGAAGTTCATGTTAATA
TGGCATTGATTGCTACAGTGGGAAACGGAATGAATAGAACTAAGGGCATATCTGCAAAGATATTTAAAGGACTTTTAGAG
GAGGATGTAAATATAAGAATGATTAATCAAGGTTCTAGTGAAATAAATATTATTGTGGGTGTAGAAAATGATGACTTTGA
AAGGGCTATAAGGGGTATATATAATACTTTTATAAAATAG

Upstream 100 bases:

>100_bases
AATTTAAAAAATTAGATAGTTTAGAGTAAGAAATGAAAGGATCTTTAATATATTGATTACTGTGAATTATTAATCAGATA
AACAGGGGGCTTAAAATAAA

Downstream 100 bases:

>100_bases
CTAAAAAACTGTTTAGAAATATTAATAATTTATCCTATTAGTATTTTTAACATTGTTAGTTTTAAAAGTTATAGATATAA
AAATTCCTGAGATTTAGAGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MGKVIVAKFGGSSLSDSEQFRKVKSIIYDNENRRYIVPSAPGKRFKKDYKITDLLYLCHAHRESAISFDDVFKHIEERYL
NLAKELRVKVDIKNKLEEIKREIEAGASRDFAASRGEYLNGLILADYLNYEFIDAAEIIHFKKYGSLDLRKTEKAIKEKI
KNVKKAVIPGFYGALPDGTIKTFSRGGSDVTGAIVARAVEASLYENWTDVSGFLMADPNIINDPKPIEVISYKELRELSY
MGAKVLHEESIFPVRDVKIPINIKNTNRAEDKGTLIVDDDKALNYTGSITGIAGKKGFTVIAIHKMLMNSELGFCRKLLS
ILEENGVAFENIPSGIDSVSLVIEDSQLGNKLDIILEEIKRQCNPDSLEVHVNMALIATVGNGMNRTKGISAKIFKGLLE
EDVNIRMINQGSSEINIIVGVENDDFERAIRGIYNTFIK

Sequences:

>Translated_439_residues
MGKVIVAKFGGSSLSDSEQFRKVKSIIYDNENRRYIVPSAPGKRFKKDYKITDLLYLCHAHRESAISFDDVFKHIEERYL
NLAKELRVKVDIKNKLEEIKREIEAGASRDFAASRGEYLNGLILADYLNYEFIDAAEIIHFKKYGSLDLRKTEKAIKEKI
KNVKKAVIPGFYGALPDGTIKTFSRGGSDVTGAIVARAVEASLYENWTDVSGFLMADPNIINDPKPIEVISYKELRELSY
MGAKVLHEESIFPVRDVKIPINIKNTNRAEDKGTLIVDDDKALNYTGSITGIAGKKGFTVIAIHKMLMNSELGFCRKLLS
ILEENGVAFENIPSGIDSVSLVIEDSQLGNKLDIILEEIKRQCNPDSLEVHVNMALIATVGNGMNRTKGISAKIFKGLLE
EDVNIRMINQGSSEINIIVGVENDDFERAIRGIYNTFIK
>Mature_438_residues
GKVIVAKFGGSSLSDSEQFRKVKSIIYDNENRRYIVPSAPGKRFKKDYKITDLLYLCHAHRESAISFDDVFKHIEERYLN
LAKELRVKVDIKNKLEEIKREIEAGASRDFAASRGEYLNGLILADYLNYEFIDAAEIIHFKKYGSLDLRKTEKAIKEKIK
NVKKAVIPGFYGALPDGTIKTFSRGGSDVTGAIVARAVEASLYENWTDVSGFLMADPNIINDPKPIEVISYKELRELSYM
GAKVLHEESIFPVRDVKIPINIKNTNRAEDKGTLIVDDDKALNYTGSITGIAGKKGFTVIAIHKMLMNSELGFCRKLLSI
LEENGVAFENIPSGIDSVSLVIEDSQLGNKLDIILEEIKRQCNPDSLEVHVNMALIATVGNGMNRTKGISAKIFKGLLEE
DVNIRMINQGSSEINIIVGVENDDFERAIRGIYNTFIK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=466, Percent_Identity=28.9699570815451, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790455, Length=474, Percent_Identity=25.9493670886076, Blast_Score=131, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320893, Length=495, Percent_Identity=26.4646464646465, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49137; Mature: 49006

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVIVAKFGGSSLSDSEQFRKVKSIIYDNENRRYIVPSAPGKRFKKDYKITDLLYLCHA
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHH
HRESAISFDDVFKHIEERYLNLAKELRVKVDIKNKLEEIKREIEAGASRDFAASRGEYLN
HHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCHHHC
GLILADYLNYEFIDAAEIIHFKKYGSLDLRKTEKAIKEKIKNVKKAVIPGFYGALPDGTI
CEEEEHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCH
KTFSRGGSDVTGAIVARAVEASLYENWTDVSGFLMADPNIINDPKPIEVISYKELRELSY
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHH
MGAKVLHEESIFPVRDVKIPINIKNTNRAEDKGTLIVDDDKALNYTGSITGIAGKKGFTV
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCEEECCCEEEECCCCCCCH
IAIHKMLMNSELGFCRKLLSILEENGVAFENIPSGIDSVSLVIEDSQLGNKLDIILEEIK
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
RQCNPDSLEVHVNMALIATVGNGMNRTKGISAKIFKGLLEEDVNIRMINQGSSEINIIVG
HHCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
VENDDFERAIRGIYNTFIK
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKVIVAKFGGSSLSDSEQFRKVKSIIYDNENRRYIVPSAPGKRFKKDYKITDLLYLCHA
CCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHH
HRESAISFDDVFKHIEERYLNLAKELRVKVDIKNKLEEIKREIEAGASRDFAASRGEYLN
HHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCCCHHCCCHHHC
GLILADYLNYEFIDAAEIIHFKKYGSLDLRKTEKAIKEKIKNVKKAVIPGFYGALPDGTI
CEEEEHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCH
KTFSRGGSDVTGAIVARAVEASLYENWTDVSGFLMADPNIINDPKPIEVISYKELRELSY
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHH
MGAKVLHEESIFPVRDVKIPINIKNTNRAEDKGTLIVDDDKALNYTGSITGIAGKKGFTV
HHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCEEECCCEEEECCCCCCCH
IAIHKMLMNSELGFCRKLLSILEENGVAFENIPSGIDSVSLVIEDSQLGNKLDIILEEIK
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
RQCNPDSLEVHVNMALIATVGNGMNRTKGISAKIFKGLLEEDVNIRMINQGSSEINIIVG
HHCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEE
VENDDFERAIRGIYNTFIK
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]