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The map label for this gene is lplA [H]

Identifier: 153935051

GI number: 153935051

Start: 1745011

End: 1746006

Strand: Direct

Name: lplA [H]

Synonym: CLC_1661

Alternate gene names: 153935051

Gene position: 1745011-1746006 (Clockwise)

Preceding gene: 153937778

Following gene: 153935704

Centisome position: 46.4

GC content: 25.4

Gene sequence:

>996_bases
ATGCAGTTTTTAGTAAATAAATCAACAAATCCTTTTTTCAATTTGGCTTTAGAAGAATATCTTTTAAAAAATGTAGATAT
TAAGGAAGACTATTTTATTCTCTGGCAAAATGAGCCTACAATTGTAATAGGAAAACATCAAAATACATTAAAGGAAATTA
ATATGAATTTTGTGCAAGATAATAATATAAATGTAGTTAGAAGGAATTCTGGAGGAGGAGCTGTATATCACGATTTAGGT
AATATAAATTTTACCTTCATAACTAAATATGATGAAAAACATTTGCTTGATTTTAAAACCTTTACCAATCCTGTAGTTTA
TTCTTTAGGTAAACTCAATGTAAAAGCAGAATTATCAGGAAGAAATGATATTTTAATAGATGGAAGAAAAATATCAGGAA
ATTCTCAACATATATATAAAGATAGATTTCTTCATCACGGAACTTTATTATTTAATTCTGAACTAGAAAATTTAGTTAAA
GCCTTAAATGTAGACAATGATAAAATACTATCTAAGGGTATTGAATCAATAAAAAGTAGAGTAACAAACATTAAAGAACA
TGTAAAAGAAGATATTTTTATGGAAGAATTTAAAGAAATACTTATAGAAAATATTTTTATATGGAATAAAAGTTCTCTTA
AAGAGTACAATTTGACTAGTGACCATATTAATGGGATAGAAAAATTAATGAAAGAAAAGTACATGACCTGGCAATGGAAC
TATGGTGAGTCACCAGAATTTAACTACAGAAATTCTAAAAGATTTCAAGGTGGTAAGTTTGAAGTATTATTAAATATTGT
AGAAGGACATATTAATGAATGTAAAATATATGGTGACTTTTTAGGATTAATGGATGTATCGGAAATAGAGAAAAAAATTA
TTGGGGTAAAATATGGGGAAGAATATATAGATGAATTTTTAAAAGGAATTGATATAAAGAAGTATTTTGGTACTATATCC
TTTGATGAAATAAAATCCTGTTTCGTAGAACTTTAA

Upstream 100 bases:

>100_bases
TCCAACAATAGGAGAAGCAATGAAGGAGGCAGCTCTTGCAGTAAACAAAGAAGCAATTCATATGGTGAATAAATAAAATC
AAGTTATGGAGGTAAAAATA

Downstream 100 bases:

>100_bases
GAGATTAGTTAAGGATCTGATGTATACAATAGTTAATATAAAGTGATTTTTAGATTTAGGTGAGGAATATATTTCTCTTA
TTAAATATTAGAAAATTTAT

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKEDIFMEEFKEILIENIFIWNKSSLKEYNLTSDHINGIEKLMKEKYMTWQWN
YGESPEFNYRNSKRFQGGKFEVLLNIVEGHINECKIYGDFLGLMDVSEIEKKIIGVKYGEEYIDEFLKGIDIKKYFGTIS
FDEIKSCFVEL

Sequences:

>Translated_331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKEDIFMEEFKEILIENIFIWNKSSLKEYNLTSDHINGIEKLMKEKYMTWQWN
YGESPEFNYRNSKRFQGGKFEVLLNIVEGHINECKIYGDFLGLMDVSEIEKKIIGVKYGEEYIDEFLKGIDIKKYFGTIS
FDEIKSCFVEL
>Mature_331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKEDIFMEEFKEILIENIFIWNKSSLKEYNLTSDHINGIEKLMKEKYMTWQWN
YGESPEFNYRNSKRFQGGKFEVLLNIVEGHINECKIYGDFLGLMDVSEIEKKIIGVKYGEEYIDEFLKGIDIKKYFGTIS
FDEIKSCFVEL

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=313, Percent_Identity=31.3099041533546, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21729884, Length=313, Percent_Identity=31.3099041533546, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21729882, Length=313, Percent_Identity=31.3099041533546, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21729880, Length=313, Percent_Identity=31.3099041533546, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21729878, Length=313, Percent_Identity=31.3099041533546, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1790846, Length=313, Percent_Identity=34.8242811501597, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=31.8897637795276, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=187, Percent_Identity=34.7593582887701, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28573464, Length=328, Percent_Identity=28.9634146341463, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38691; Mature: 38691

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQD
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHHHCCCEEEEC
NNINVVRRNSGGGAVYHDLGNINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSG
CCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCEEEHHHHCCHHHHHHCCEEEEEEECC
RNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVKALNVDNDKILSKGIESIKSR
CCCEEEECEEECCCCCEEHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTNIKEHVKEDIFMEEFKEILIENIFIWNKSSLKEYNLTSDHINGIEKLMKEKYMTWQWN
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
YGESPEFNYRNSKRFQGGKFEVLLNIVEGHINECKIYGDFLGLMDVSEIEKKIIGVKYGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCCH
EYIDEFLKGIDIKKYFGTISFDEIKSCFVEL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQD
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHHHCCCEEEEC
NNINVVRRNSGGGAVYHDLGNINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSG
CCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCEEEHHHHCCHHHHHHCCEEEEEEECC
RNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVKALNVDNDKILSKGIESIKSR
CCCEEEECEEECCCCCEEHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTNIKEHVKEDIFMEEFKEILIENIFIWNKSSLKEYNLTSDHINGIEKLMKEKYMTWQWN
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
YGESPEFNYRNSKRFQGGKFEVLLNIVEGHINECKIYGDFLGLMDVSEIEKKIIGVKYGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCCH
EYIDEFLKGIDIKKYFGTISFDEIKSCFVEL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]