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The map label for this gene is aroE

Identifier: 153934143

GI number: 153934143

Start: 2550678

End: 2551454

Strand: Reverse

Name: aroE

Synonym: CLB_2416

Alternate gene names: 153934143

Gene position: 2551454-2550678 (Counterclockwise)

Preceding gene: 153933487

Following gene: 153933344

Centisome position: 66.04

GC content: 25.1

Gene sequence:

>777_bases
ATGTATACAACAGGTTTAATAGGTAAAAATATTAATTATTCAGAGTCACCAGAAATACACAATAATTATTACAAAAAAAA
TAATATACCATTCTTTTATAAAATATTTAATTTAAAGCAGGATCAAATTGATGATTTTATAAAGAACTTACATAAGAATA
ATATAAAAGGATTTAATGTTACTATTCCATATAAAGAAACAATATTACAATATTTAAATGATATAGTATATCCAGCAGAT
AAGATAGGAGCAGTGAATACTGTAGCAGTACAGGAAGATAAACTAATAGGGTATAATACAGACTATATAGGATTTATAAA
AAGTTTACAATATTACAATATACAGGTTAAAAATTTTAAGTGTCTCATTATAGGATCAGGTGGAAGTGCCAAGTGTATAT
ATTATGCATTAAAAGAATTAAATGCAAGAGATATATGTATAGTTTCTAGAAATCCTGAGAAAGCAAGACTAAAATTTGAA
AAAAAAGTTAAAATATTAAATATTAAAGACGAAAATAAATTAGATAGATATGATTTAATTGTAAACTGTACACCCATAGG
CGGACCTAATCTTAAAGAACAAAAACCTATAGAACTTAAAGAGATCAAAAAAAATTGTGTGGTTTATGATTTGAATTATA
CTCCTAAAAGATCTAAATTATTAAAAGAAGCTAAAGAAAATGGGGCATTTATTATAAATGGAGAAAAAATGCTCATTTTT
CAGGCCTATAGTGCTATAGGTCTTTGGTGCTTAAATGGTATTAAAGGAGGACGATAA

Upstream 100 bases:

>100_bases
GGTGATTCTTAATTCTTTTGTTTTATTTTTTATTTATTTTATGGCAGGAGATTCTTTACAGAATATTTATAATCGTTATA
ATATAAGCAGGAGGGTTTAA

Downstream 100 bases:

>100_bases
ATTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCGGTAGGGGTAGAAC
CAACTATAAGAATAACTGGA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MYTTGLIGKNINYSESPEIHNNYYKKNNIPFFYKIFNLKQDQIDDFIKNLHKNNIKGFNVTIPYKETILQYLNDIVYPAD
KIGAVNTVAVQEDKLIGYNTDYIGFIKSLQYYNIQVKNFKCLIIGSGGSAKCIYYALKELNARDICIVSRNPEKARLKFE
KKVKILNIKDENKLDRYDLIVNCTPIGGPNLKEQKPIELKEIKKNCVVYDLNYTPKRSKLLKEAKENGAFIINGEKMLIF
QAYSAIGLWCLNGIKGGR

Sequences:

>Translated_258_residues
MYTTGLIGKNINYSESPEIHNNYYKKNNIPFFYKIFNLKQDQIDDFIKNLHKNNIKGFNVTIPYKETILQYLNDIVYPAD
KIGAVNTVAVQEDKLIGYNTDYIGFIKSLQYYNIQVKNFKCLIIGSGGSAKCIYYALKELNARDICIVSRNPEKARLKFE
KKVKILNIKDENKLDRYDLIVNCTPIGGPNLKEQKPIELKEIKKNCVVYDLNYTPKRSKLLKEAKENGAFIINGEKMLIF
QAYSAIGLWCLNGIKGGR
>Mature_258_residues
MYTTGLIGKNINYSESPEIHNNYYKKNNIPFFYKIFNLKQDQIDDFIKNLHKNNIKGFNVTIPYKETILQYLNDIVYPAD
KIGAVNTVAVQEDKLIGYNTDYIGFIKSLQYYNIQVKNFKCLIIGSGGSAKCIYYALKELNARDICIVSRNPEKARLKFE
KKVKILNIKDENKLDRYDLIVNCTPIGGPNLKEQKPIELKEIKKNCVVYDLNYTPKRSKLLKEAKENGAFIINGEKMLIF
QAYSAIGLWCLNGIKGGR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=260, Percent_Identity=25.7692307692308, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789675, Length=259, Percent_Identity=30.8880308880309, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320332, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_CLOB1 (A7FWA9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384720.1
- ProteinModelPortal:   A7FWA9
- SMR:   A7FWA9
- STRING:   A7FWA9
- GeneID:   5395119
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2416
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   TLFMKKG
- ProtClustDB:   CLSK912105
- BioCyc:   CBOT441770:CLB_2416-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29691; Mature: 29691

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTTGLIGKNINYSESPEIHNNYYKKNNIPFFYKIFNLKQDQIDDFIKNLHKNNIKGFNV
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEE
TIPYKETILQYLNDIVYPADKIGAVNTVAVQEDKLIGYNTDYIGFIKSLQYYNIQVKNFK
EECHHHHHHHHHHHHCCCCHHCCCEEEEEEECCCEEECCCHHHHHHHCEEEEEEEECCEE
CLIIGSGGSAKCIYYALKELNARDICIVSRNPEKARLKFEKKVKILNIKDENKLDRYDLI
EEEEECCCCCEEHEEEHHHCCCCEEEEEECCCCHHHEEEECCEEEEEECCCCCCCEEEEE
VNCTPIGGPNLKEQKPIELKEIKKNCVVYDLNYTPKRSKLLKEAKENGAFIINGEKMLIF
EEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCEEEEE
QAYSAIGLWCLNGIKGGR
EEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYTTGLIGKNINYSESPEIHNNYYKKNNIPFFYKIFNLKQDQIDDFIKNLHKNNIKGFNV
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEE
TIPYKETILQYLNDIVYPADKIGAVNTVAVQEDKLIGYNTDYIGFIKSLQYYNIQVKNFK
EECHHHHHHHHHHHHCCCCHHCCCEEEEEEECCCEEECCCHHHHHHHCEEEEEEEECCEE
CLIIGSGGSAKCIYYALKELNARDICIVSRNPEKARLKFEKKVKILNIKDENKLDRYDLI
EEEEECCCCCEEHEEEHHHCCCCEEEEEECCCCHHHEEEECCEEEEEECCCCCCCEEEEE
VNCTPIGGPNLKEQKPIELKEIKKNCVVYDLNYTPKRSKLLKEAKENGAFIINGEKMLIF
EEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCEEEEE
QAYSAIGLWCLNGIKGGR
EEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA