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The map label for this gene is pilT [H]

Identifier: 153933344

GI number: 153933344

Start: 2549624

End: 2550676

Strand: Reverse

Name: pilT [H]

Synonym: CLB_2415

Alternate gene names: 153933344

Gene position: 2550676-2549624 (Counterclockwise)

Preceding gene: 153934143

Following gene: 153931104

Centisome position: 66.02

GC content: 29.72

Gene sequence:

>1053_bases
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCGGTAGGGGTAGAACC
AACTATAAGAATAACTGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCCTAGGTAGA
TTCAGAGTAAGCGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTGAAGATCCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGCAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Upstream 100 bases:

>100_bases
AATGGGGCATTTATTATAAATGGAGAAAAAATGCTCATTTTTCAGGCCTATAGTGCTATAGGTCTTTGGTGCTTAAATGG
TATTAAAGGAGGACGATAAA

Downstream 100 bases:

>100_bases
AAATATTATGATATTAAAATATAGAATAATAATTATGCATTTGGTAATATTAAAAAAAGGACCTTTTGGTCCTTTTTTTA
AAATATCATATAATCTAACA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Sequences:

>Translated_350_residues
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL
>Mature_350_residues
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=44.2367601246106, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1786296, Length=200, Percent_Identity=38.5, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1789723, Length=163, Percent_Identity=41.1042944785276, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39042; Mature: 39042

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEF
CHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDLLKKGEIDTSYSISGLGRFRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTN
HHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHC
KKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLEDPIEFLHKHNKCIINQREIGR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCEEECHHHHCC
DTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHH
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
IESSIQTGSKYGMKTMDMSLAELYKREIISYDTALNYSVDKDLITKIINL
EECCHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKQLKELLEKTINFGASDLHLSVGVEPTIRITGQLSKIFKEKLTPEDTNNYAKEILGEEF
CHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EDLLKKGEIDTSYSISGLGRFRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTN
HHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHC
KKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLEDPIEFLHKHNKCIINQREIGR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCEEECHHHHCC
DTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHH
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQ
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEE
IESSIQTGSKYGMKTMDMSLAELYKREIISYDTALNYSVDKDLITKIINL
EECCHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]