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The map label for this gene is priA [H]

Identifier: 153933517

GI number: 153933517

Start: 2518578

End: 2520785

Strand: Reverse

Name: priA [H]

Synonym: CLB_2384

Alternate gene names: 153933517

Gene position: 2520785-2518578 (Counterclockwise)

Preceding gene: 153931189

Following gene: 153931783

Centisome position: 65.25

GC content: 26.59

Gene sequence:

>2208_bases
GTGTTTAAGTATGCAGGTATTATAGTAAATAATTCTTCTATAAAATTAGATAAATTATTTACTTATGCTATTCCAGAGGA
ATTAAATAAAAAAATAAGAATAGGACATAGAGTAAAAGTTCCCTTCGGTAAAGGTAATAAAAAAATAGATGGTTTTGTTT
TAAAACTATATGAAAACTATGAAGATGAAAATAAAGTTTCTATAAAATCTATAAGCAATATATGTGAAGAGGATCCTCTA
TTAGATTTGAATAGAGTAAATCTTGTAGAACAAATGAGGGAAAGATATCTTTGTACATATTTAGAAGCTATAAAAGTATT
AGTGCCACCAGGTATTACAAAGGGAAACAAAGAAAAAAAAGAAAATTTTATATTTATATATAAAGATTTAGAGGGAAAAT
ATTTAAAAGAAAATTATGAGCAAATATATAACTTCGTAAAATTAAATGATGGTGTTTATAATAAAAATGTATTAAATAAA
AAATTTGGATTTTCCTTATCTTCCATAAATACATTAATAAAACATGGATTTTTAATTCAAAAGAACCAAGTAGTAAATAG
ATATAATGAAAAAATATATGGAAATTATGAAGAAAAAATACTAAATGGAGACCAAAGTTTAGCTCTTTATAGAATTTTAA
ATTCCAATAGAAAGTTATTTTTAATACATGGTGTTACAGGTAGTGGAAAGACAGAGATATACATGCATTTAGTAAAAAAA
TTAATAGAAAAAGATAAGGATTCTATAATTTTAGTACCAGAAATATCACTAACTCCTCAGATGGTGGAAAGATTCAAAGG
TAGATTTGGGAAAAGTATAGCTATATTTCATAGCAAACTCTCTAATGGTGAAAGATTTGATGAATGGATGAGAGTAAAAA
GAGGAGAAGTTAAAGTAGCTATAGGGGCAAGATCAGCAGTGTTTTTACCTTTTAAAAATTTAGGTGCTATAATAATAGAC
GAAGAACATGAAAGTAGCTATAAATCTGATAGTAATCCTAAATATAATGCTAGAGATATAGCAGAACTTAGATGCATGAA
TGAAGGCTGTAAGGTCATTTTAGGATCGGCTACACCATCTTTGGAAACTTATTATAGAAGTAAAAATAAAATAATAGATT
TAATCACAATAAAGGAAAGAATAGAAGGGGCATCTATGCCAGAGGTTAAAATAGTTGATATGAGAGAGGAACTTATGTCA
GGTAATAAATCTATATTTAGCAGGGAACTATTTGAAGATATAAGTACAAGGTTAGAAAAAAAAGAGCAAATAATATTATT
TTTAAATAGAAGAGGTTTTTCTACCTTTGTATCCTGTAGAGAATGTGGGTATGTTTTTAAATGTAAAAATTGTGATATTT
CTTTAACCTATCATAATAAAGATAATAGCTTAAGATGCCATTATTGTGGAGCAAGCACGAGAATACCAAAACAATGTCCT
AAATGCAATAGTAAATATGTAAAGTATTTTGGAGCAGGTACAGAAAAAGTGGAAAAAGCTATAAAAAAATGTTTTCCTAA
AGTTAAAACTTTAAGAATGGATTTGGATACCACAAGAACTAAGGATGCTTATGAAAATATTTACAATACATTTAAAAAGG
GTGAAGCTGATATATTAATTGGAACTCAAATGATAGCAAAAGGATTAGATTTTGAAAATGTAACTTTAGTTGGAGTTTTA
GCAGCGGATATGTCTTTAAATTTACCAGACTATAGATCAGGAGAAAGAACTTTTCAGCTTATAACTCAAGTTTCAGGAAG
GGCAGGAAGAGGAAAGAAAGAAGGAAAAGTTTTAATTCAAAGTTATAATCCAGAAAATTATAGTATAGCTTCCGCGGCTA
AAAGTGATTATGAAGGATTTTATAATAAAGAGATAAAAATAAGAAGAGATATGAATTATCCTCCCTTTTCAAAAATATTG
CTTATAAATTTAAGCAGTAAATATGAGAATAAATTAATAAAAAATACACATAAAGTTGGTATAATATTAAAGAATGTTTT
ATCTAAATATGATAACTTAAATATGTTAGGTCCTTGTCCTAGTCCATTATCTAAAATAAAAGAGATGTATAGATGGCAAA
TAATATTAAAAGGAAATGTGACGGGAGATATAATGAAGGAAATACAAAAAATAGTTTATACTTCTTTAGAACAAGTTTAT
AATGATATAAGAGTAAGTATAGATATAAATCCGAACAGTTTGTTATAA

Upstream 100 bases:

>100_bases
ATTCAGGGACATACCCGCTCTTTACTCCCACTTTGAAGAAGATGGGAGTATTAGAGCGGGTAGTCATCGGATAAAAAAAT
TTCCTTAAAAGGGTGATAAA

Downstream 100 bases:

>100_bases
TTTTTTAAATTTTAGGAGGTCGTAAAAATGGCAATAAGAAATATAAGAAAATATGGAGATGAATTATTAAGGAAGAAAAG
CAGGAAAATAGAAAAGATTG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MFKYAGIIVNNSSIKLDKLFTYAIPEELNKKIRIGHRVKVPFGKGNKKIDGFVLKLYENYEDENKVSIKSISNICEEDPL
LDLNRVNLVEQMRERYLCTYLEAIKVLVPPGITKGNKEKKENFIFIYKDLEGKYLKENYEQIYNFVKLNDGVYNKNVLNK
KFGFSLSSINTLIKHGFLIQKNQVVNRYNEKIYGNYEEKILNGDQSLALYRILNSNRKLFLIHGVTGSGKTEIYMHLVKK
LIEKDKDSIILVPEISLTPQMVERFKGRFGKSIAIFHSKLSNGERFDEWMRVKRGEVKVAIGARSAVFLPFKNLGAIIID
EEHESSYKSDSNPKYNARDIAELRCMNEGCKVILGSATPSLETYYRSKNKIIDLITIKERIEGASMPEVKIVDMREELMS
GNKSIFSRELFEDISTRLEKKEQIILFLNRRGFSTFVSCRECGYVFKCKNCDISLTYHNKDNSLRCHYCGASTRIPKQCP
KCNSKYVKYFGAGTEKVEKAIKKCFPKVKTLRMDLDTTRTKDAYENIYNTFKKGEADILIGTQMIAKGLDFENVTLVGVL
AADMSLNLPDYRSGERTFQLITQVSGRAGRGKKEGKVLIQSYNPENYSIASAAKSDYEGFYNKEIKIRRDMNYPPFSKIL
LINLSSKYENKLIKNTHKVGIILKNVLSKYDNLNMLGPCPSPLSKIKEMYRWQIILKGNVTGDIMKEIQKIVYTSLEQVY
NDIRVSIDINPNSLL

Sequences:

>Translated_735_residues
MFKYAGIIVNNSSIKLDKLFTYAIPEELNKKIRIGHRVKVPFGKGNKKIDGFVLKLYENYEDENKVSIKSISNICEEDPL
LDLNRVNLVEQMRERYLCTYLEAIKVLVPPGITKGNKEKKENFIFIYKDLEGKYLKENYEQIYNFVKLNDGVYNKNVLNK
KFGFSLSSINTLIKHGFLIQKNQVVNRYNEKIYGNYEEKILNGDQSLALYRILNSNRKLFLIHGVTGSGKTEIYMHLVKK
LIEKDKDSIILVPEISLTPQMVERFKGRFGKSIAIFHSKLSNGERFDEWMRVKRGEVKVAIGARSAVFLPFKNLGAIIID
EEHESSYKSDSNPKYNARDIAELRCMNEGCKVILGSATPSLETYYRSKNKIIDLITIKERIEGASMPEVKIVDMREELMS
GNKSIFSRELFEDISTRLEKKEQIILFLNRRGFSTFVSCRECGYVFKCKNCDISLTYHNKDNSLRCHYCGASTRIPKQCP
KCNSKYVKYFGAGTEKVEKAIKKCFPKVKTLRMDLDTTRTKDAYENIYNTFKKGEADILIGTQMIAKGLDFENVTLVGVL
AADMSLNLPDYRSGERTFQLITQVSGRAGRGKKEGKVLIQSYNPENYSIASAAKSDYEGFYNKEIKIRRDMNYPPFSKIL
LINLSSKYENKLIKNTHKVGIILKNVLSKYDNLNMLGPCPSPLSKIKEMYRWQIILKGNVTGDIMKEIQKIVYTSLEQVY
NDIRVSIDINPNSLL
>Mature_735_residues
MFKYAGIIVNNSSIKLDKLFTYAIPEELNKKIRIGHRVKVPFGKGNKKIDGFVLKLYENYEDENKVSIKSISNICEEDPL
LDLNRVNLVEQMRERYLCTYLEAIKVLVPPGITKGNKEKKENFIFIYKDLEGKYLKENYEQIYNFVKLNDGVYNKNVLNK
KFGFSLSSINTLIKHGFLIQKNQVVNRYNEKIYGNYEEKILNGDQSLALYRILNSNRKLFLIHGVTGSGKTEIYMHLVKK
LIEKDKDSIILVPEISLTPQMVERFKGRFGKSIAIFHSKLSNGERFDEWMRVKRGEVKVAIGARSAVFLPFKNLGAIIID
EEHESSYKSDSNPKYNARDIAELRCMNEGCKVILGSATPSLETYYRSKNKIIDLITIKERIEGASMPEVKIVDMREELMS
GNKSIFSRELFEDISTRLEKKEQIILFLNRRGFSTFVSCRECGYVFKCKNCDISLTYHNKDNSLRCHYCGASTRIPKQCP
KCNSKYVKYFGAGTEKVEKAIKKCFPKVKTLRMDLDTTRTKDAYENIYNTFKKGEADILIGTQMIAKGLDFENVTLVGVL
AADMSLNLPDYRSGERTFQLITQVSGRAGRGKKEGKVLIQSYNPENYSIASAAKSDYEGFYNKEIKIRRDMNYPPFSKIL
LINLSSKYENKLIKNTHKVGIILKNVLSKYDNLNMLGPCPSPLSKIKEMYRWQIILKGNVTGDIMKEIQKIVYTSLEQVY
NDIRVSIDINPNSLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=703, Percent_Identity=33.0014224751067, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84503; Mature: 84503

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKYAGIIVNNSSIKLDKLFTYAIPEELNKKIRIGHRVKVPFGKGNKKIDGFVLKLYENY
CCEEEEEEEECCCEEEHHHEEECCCHHCCCCEECCEEEEECCCCCCCCCCCEEEEEECCC
EDENKVSIKSISNICEEDPLLDLNRVNLVEQMRERYLCTYLEAIKVLVPPGITKGNKEKK
CCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ENFIFIYKDLEGKYLKENYEQIYNFVKLNDGVYNKNVLNKKFGFSLSSINTLIKHGFLIQ
CCEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHHHCCEEEE
KNQVVNRYNEKIYGNYEEKILNGDQSLALYRILNSNRKLFLIHGVTGSGKTEIYMHLVKK
HHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
LIEKDKDSIILVPEISLTPQMVERFKGRFGKSIAIFHSKLSNGERFDEWMRVKRGEVKVA
HHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHCCCCEEEE
IGARSAVFLPFKNLGAIIIDEEHESSYKSDSNPKYNARDIAELRCMNEGCKVILGSATPS
EECCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
LETYYRSKNKIIDLITIKERIEGASMPEVKIVDMREELMSGNKSIFSRELFEDISTRLEK
HHHHHCCCCCEEEEEEEHHHHCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCC
KEQIILFLNRRGFSTFVSCRECGYVFKCKNCDISLTYHNKDNSLRCHYCGASTRIPKQCP
CCEEEEEEECCCCHHHHHHHHCCCEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
KCNSKYVKYFGAGTEKVEKAIKKCFPKVKTLRMDLDTTRTKDAYENIYNTFKKGEADILI
CCCCCHHHEECCCHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
GTQMIAKGLDFENVTLVGVLAADMSLNLPDYRSGERTFQLITQVSGRAGRGKKEGKVLIQ
EHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
SYNPENYSIASAAKSDYEGFYNKEIKIRRDMNYPPFSKILLINLSSKYENKLIKNTHKVG
ECCCCCCEEHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
IILKNVLSKYDNLNMLGPCPSPLSKIKEMYRWQIILKGNVTGDIMKEIQKIVYTSLEQVY
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
NDIRVSIDINPNSLL
CCEEEEEEECCCCCC
>Mature Secondary Structure
MFKYAGIIVNNSSIKLDKLFTYAIPEELNKKIRIGHRVKVPFGKGNKKIDGFVLKLYENY
CCEEEEEEEECCCEEEHHHEEECCCHHCCCCEECCEEEEECCCCCCCCCCCEEEEEECCC
EDENKVSIKSISNICEEDPLLDLNRVNLVEQMRERYLCTYLEAIKVLVPPGITKGNKEKK
CCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ENFIFIYKDLEGKYLKENYEQIYNFVKLNDGVYNKNVLNKKFGFSLSSINTLIKHGFLIQ
CCEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCHHHCCCHHHHHHHHHCCEEEE
KNQVVNRYNEKIYGNYEEKILNGDQSLALYRILNSNRKLFLIHGVTGSGKTEIYMHLVKK
HHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
LIEKDKDSIILVPEISLTPQMVERFKGRFGKSIAIFHSKLSNGERFDEWMRVKRGEVKVA
HHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHCCCCEEEE
IGARSAVFLPFKNLGAIIIDEEHESSYKSDSNPKYNARDIAELRCMNEGCKVILGSATPS
EECCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
LETYYRSKNKIIDLITIKERIEGASMPEVKIVDMREELMSGNKSIFSRELFEDISTRLEK
HHHHHCCCCCEEEEEEEHHHHCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCC
KEQIILFLNRRGFSTFVSCRECGYVFKCKNCDISLTYHNKDNSLRCHYCGASTRIPKQCP
CCEEEEEEECCCCHHHHHHHHCCCEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCC
KCNSKYVKYFGAGTEKVEKAIKKCFPKVKTLRMDLDTTRTKDAYENIYNTFKKGEADILI
CCCCCHHHEECCCHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
GTQMIAKGLDFENVTLVGVLAADMSLNLPDYRSGERTFQLITQVSGRAGRGKKEGKVLIQ
EHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
SYNPENYSIASAAKSDYEGFYNKEIKIRRDMNYPPFSKILLINLSSKYENKLIKNTHKVG
ECCCCCCEEHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
IILKNVLSKYDNLNMLGPCPSPLSKIKEMYRWQIILKGNVTGDIMKEIQKIVYTSLEQVY
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
NDIRVSIDINPNSLL
CCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]