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The map label for this gene is bacA [H]

Identifier: 153933149

GI number: 153933149

Start: 2644374

End: 2646203

Strand: Reverse

Name: bacA [H]

Synonym: CLB_2528

Alternate gene names: 153933149

Gene position: 2646203-2644374 (Counterclockwise)

Preceding gene: 153932164

Following gene: 153931655

Centisome position: 68.49

GC content: 25.96

Gene sequence:

>1830_bases
ATGAGCTATAACTATTTTGAAAAACAACAAAGAGAAGATTCTATATTGGTTGGACAGGATATTTTAAGTTTTAGATATAA
TAATAGCAGAATAGATACTACAATAATTTCTTGCTTAAGAAAAATCGTTAACTTAAGTAAAGAAAAAGTAGCTATTATAG
ATAATGAAACAAAGATAACTTATGGACATTTCATGAAGCGAATTAATGCTCTATCAAATTTTTTGCTGAATCAAAAGGAG
TTTGAAAGAACATCACCAATTACTTTAATTTTAAATGGGAATTATTTAAGATATGTTTGCGTATATGGTGTTTTAGCTTC
AGGTGGGTATTATTTGCCTATAGAACCAGAAGTAAACAATATTAATAGAATAATTTCTTTAGTAGAACGTTCTAAAAGTA
AAGTTGTAATTACAAATAGAGAGTATGTAAATATATTAAAAGAAAAAATATCAGAAAGTGTAAGTTTTATAATTATAGAA
GATTTAGATTTTAGTAGCCTAGATAGCAAAGAAAACGTTATTGATATAAAAGCTGATGATCCAGCATATATGATATATAC
ATCTGGATCTACGGGAGTACCTAAAGGTGTTATTATACCTCATAGAGCTGTTGTGAATTTAGTTAATGCATTGTACGTTA
ATTTCAGATTAACAAAAAAGGATAGGGGTGTGGCATTTACATCATTTTCTTTTGATTCATGTGGTTGTGATATATATCCA
TATTTATTGAATGAAGTCCCTTTAGTATGCATTGAGAGGAGAAAAGAATATGTTTACGATATTGAAAAATTAAATAAATT
TTGTATTGATAATATGATATCAATAATGTTTTTACCTACGGTTTTAGCTGAAAAATTTATTACAGTTGATAATAATGTGC
TAAGAGTTCTTTATTTTGGAGGGGAAAAATTTTGTAAGAATAAAGTTACTAATTATGAGTTATTTAATTCTTATGGATTG
ACAGAAACAGGAATACTTAATACAGTCTATAAGATTAAAGGAGATGAGCGAGAAATTCCTTTGGGAGATCCAATAATAAA
TTCAGGAATATTAATTTTAGATGATCAAGATAAAATTATACCTAAAGGGAAATTAGGAGAAATTGCAATTTTTGGTTCTG
GTATAGCAATAGAATATGATAATAATACAGAGTTGACTAATAAAAAATTTATTAAGTTAAAAGAAATGGGTAATTTGAGA
TGTTGTAAAACTGGAGATTTGGGATATATAAATGAGAATGATAATCTATATTTTAAGGGTAGAAAAGATAAACAAGTAAA
AATAAGTGGTTACAGGATAGAGTTAGATGAGATACGATTTTATTTGCAGAAATATGCTTGCATAGAATTAAGTATTCCTA
TGGTAATAAAAAAAGGTAAATTATCTTTATTAGTTAATTTTTATGTTGCAAAAGAAGTACTTGATGAAGAATTAATTAAT
AAATTTTTAAGTAAATATTTACCTTTTTATATGGTGCCATTATGTCACATTAACATAAAAGATGTTCCATATACTACAAA
TGGAAAAGTTGATTATAGGGGGTTGCAGAAAATTTTTGAGAATAAAAAAGAAAAAGTATATCTAAAAAGTAATTTACTGT
ATAGCAAATTAGAATTAAAAGAGTTCCTAATTGATACTTATGCCAATATACTTAAAATGGATAAAGATGAAATAATGGAA
GATGGTAGTTTCTTTGAAATGGGTGGAGACTCACTTAAAGCTATGAAATTACAATGGTATATACAAGATGAATTAGGTGT
AGAAGTGGACATATCTAAAATTTATGAAGAATTCAGTATAAAAAAACTCCTAAATTTAATACTAATATGA

Upstream 100 bases:

>100_bases
TTATTTAGAAAAAAAATAGATGAAATTTTAATGATTAAAGAAATTATAAATAATAAGTAAAAATGTGAGTTATATAAAAA
TAATTAGGAGAAAAAAAAGG

Downstream 100 bases:

>100_bases
TTATATCCAAGTTATTCTATTTTCAAGGAGGTGTAATGGTGTGAGTATAGTTCGATTATTTACATTACCAGGGGCGGGTT
CTTCAGCGATAATGTATTAC

Product: putative nonribosomal peptide synthetase

Products: NA

Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKE
FERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVSFIIIE
DLDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP
YLLNEVPLVCIERRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL
TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR
CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYRGLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME
DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI

Sequences:

>Translated_609_residues
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKE
FERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVSFIIIE
DLDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP
YLLNEVPLVCIERRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL
TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR
CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYRGLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME
DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI
>Mature_608_residues
SYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKEF
ERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVSFIIIED
LDFSSLDSKENVIDIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYPY
LLNEVPLVCIERRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGLT
ETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRC
CKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELINK
FLSKYLPFYMVPLCHINIKDVPYTTNGKVDYRGLQKIFENKKEKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIMED
GSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI

Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=601, Percent_Identity=24.6256239600666, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1786801, Length=608, Percent_Identity=23.1907894736842, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI221142682, Length=453, Percent_Identity=24.0618101545254, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788107, Length=365, Percent_Identity=24.1095890410959, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=22.289156626506, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=441, Percent_Identity=25.1700680272109, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556356, Length=420, Percent_Identity=23.5714285714286, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=21.9712525667351, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=610, Percent_Identity=23.9344262295082, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=24.5283018867925, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648676, Length=576, Percent_Identity=22.5694444444444, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013624
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 70265; Mature: 70133

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT
CCCCCCHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
YGHFMKRINALSNFLLNQKEFERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN
HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCHHH
INRIISLVERSKSKVVITNREYVNILKEKISESVSFIIIEDLDFSSLDSKENVIDIKADD
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEECCC
PAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP
CEEEEEECCCCCCCCCEEECHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEHH
YLLNEVPLVCIERRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG
HHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC
GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII
CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG
CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC
RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN
CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCEEEEEHHHHHHHHHHHHHHH
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYRGLQKIFENKKEKVYLKSNLLYSKLELK
HHHHHHCHHHEEHEEEEEEECCCCCCCCEECHHHHHHHHCCCCCEEEEECCCHHHHHHHH
EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI
HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH
KKLLNLILI
HHHHHHHCC
>Mature Secondary Structure 
SYNYFEKQQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT
CCCCCHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
YGHFMKRINALSNFLLNQKEFERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN
HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCHHH
INRIISLVERSKSKVVITNREYVNILKEKISESVSFIIIEDLDFSSLDSKENVIDIKADD
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEECCC
PAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP
CEEEEEECCCCCCCCCEEECHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEHH
YLLNEVPLVCIERRKEYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG
HHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC
GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII
CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG
CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC
RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN
CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCEEEEEHHHHHHHHHHHHHHH
KFLSKYLPFYMVPLCHINIKDVPYTTNGKVDYRGLQKIFENKKEKVYLKSNLLYSKLELK
HHHHHHCHHHEEHEEEEEEECCCCCCCCEECHHHHHHHHCCCCCEEEEECCCHHHHHHHH
EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI
HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH
KKLLNLILI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9427658 [H]