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The map label for this gene is ansA [H]

Identifier: 153932296

GI number: 153932296

Start: 2524503

End: 2525504

Strand: Reverse

Name: ansA [H]

Synonym: CLB_2390

Alternate gene names: 153932296

Gene position: 2525504-2524503 (Counterclockwise)

Preceding gene: 153931584

Following gene: 153931589

Centisome position: 65.37

GC content: 33.23

Gene sequence:

>1002_bases
ATGAAAAAAGTAGCAGTTGTTTTTAATGGAGGTACTATTTCCATGAAGGTAGACCCTAGAATAAAGGCTGCGGTACCAAG
TTTATCTGGGGAAGAGATCATGGCTATGGTAACGGGCATAGAAGAATATGCTGAGATAGAAAACTATGAATTTTCGAACT
ATCCAAGCCCTTATATAACTCCTGGGCAAATGATGGATTTATCAAAGTATATAAAGGACATATTAGAAAGGGAAGACATT
TGTGGAGTTGTAGTTACTCATGGTACGGATTCTTTAGAAGAAACAGCCTATCTTTTAGATTTAACTATAAAAACTCATAA
ACCTGTAATAGTTACAGGAGCTATGAGAAGCAGTTCTGAACTAGGTTATGATGGACCTGCTAATTTAGCAGCTTCCATTT
GTACAGCTATATCCAAAGAGGCTATAGGTAAAGGTGTGTTAGTTTGCTTAAATGATGAATTAAATTGTGCTAGTGAGGTT
ACAAAATCTAATTCTACTGCATTAAATACTTTTAAGAGTCCTATATTTGGACCTATAGGCATAGTAGATAATAATCAGGT
AATATTCTATAGAGAAAAACTAAAAAAACAACATATAGAGACAGATAGAATAGAAAGCAATGTAGCACTTATAAAGTGTG
TATCAGGTATAGATTCAAGTTTTATAGATTTTTGTTTGGATAGAGCTTATAAAGGTTTGGTAATAGAGGCTATGGGTAGA
GGTAATGTACCTCCTAATATGGTACCTGGTATAAAAAGAGCAATAGATAAAAATATACCAGTAGTGTTAGTTTCAAGATG
CTATGAAGGTAGAGTATTAGATACTTATGGCTATGAAGGCGGAGGAAAACAATTAAGAAAAATGGGAGTAATATTTGGGG
ATAGCCTTCCAGGACAAAAGGCAAGGATAAAATTGGCTCTAGCCTTAACAAAATCAAATTATGATGTAGAAAAAATAAGA
CAAATATTTGAAGATGGCCTTTATAAATATACCACTAAATAA

Upstream 100 bases:

>100_bases
GGAACATTTTATATTAAAAATAAAAATTTTCAATATATTTTATTATAACTAAATAATAAAAATTTAGTAGCATTAGAGAA
TAGGGGGAAGTTATGAAGAT

Downstream 100 bases:

>100_bases
ATTAAGATACTAAAAATATAATTATTTTAATAATTATAAAAATATAGTGTTATAATAATGTAAAAAGCATAAATTGTGTT
ATAATTTTATTTGGAAAACA

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKVAVVFNGGTISMKVDPRIKAAVPSLSGEEIMAMVTGIEEYAEIENYEFSNYPSPYITPGQMMDLSKYIKDILEREDI
CGVVVTHGTDSLEETAYLLDLTIKTHKPVIVTGAMRSSSELGYDGPANLAASICTAISKEAIGKGVLVCLNDELNCASEV
TKSNSTALNTFKSPIFGPIGIVDNNQVIFYREKLKKQHIETDRIESNVALIKCVSGIDSSFIDFCLDRAYKGLVIEAMGR
GNVPPNMVPGIKRAIDKNIPVVLVSRCYEGRVLDTYGYEGGGKQLRKMGVIFGDSLPGQKARIKLALALTKSNYDVEKIR
QIFEDGLYKYTTK

Sequences:

>Translated_333_residues
MKKVAVVFNGGTISMKVDPRIKAAVPSLSGEEIMAMVTGIEEYAEIENYEFSNYPSPYITPGQMMDLSKYIKDILEREDI
CGVVVTHGTDSLEETAYLLDLTIKTHKPVIVTGAMRSSSELGYDGPANLAASICTAISKEAIGKGVLVCLNDELNCASEV
TKSNSTALNTFKSPIFGPIGIVDNNQVIFYREKLKKQHIETDRIESNVALIKCVSGIDSSFIDFCLDRAYKGLVIEAMGR
GNVPPNMVPGIKRAIDKNIPVVLVSRCYEGRVLDTYGYEGGGKQLRKMGVIFGDSLPGQKARIKLALALTKSNYDVEKIR
QIFEDGLYKYTTK
>Mature_333_residues
MKKVAVVFNGGTISMKVDPRIKAAVPSLSGEEIMAMVTGIEEYAEIENYEFSNYPSPYITPGQMMDLSKYIKDILEREDI
CGVVVTHGTDSLEETAYLLDLTIKTHKPVIVTGAMRSSSELGYDGPANLAASICTAISKEAIGKGVLVCLNDELNCASEV
TKSNSTALNTFKSPIFGPIGIVDNNQVIFYREKLKKQHIETDRIESNVALIKCVSGIDSSFIDFCLDRAYKGLVIEAMGR
GNVPPNMVPGIKRAIDKNIPVVLVSRCYEGRVLDTYGYEGGGKQLRKMGVIFGDSLPGQKARIKLALALTKSNYDVEKIR
QIFEDGLYKYTTK

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=312, Percent_Identity=25.3205128205128, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789327, Length=301, Percent_Identity=32.5581395348837, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=340, Percent_Identity=26.1764705882353, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=359, Percent_Identity=25.9052924791086, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=359, Percent_Identity=25.9052924791086, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=359, Percent_Identity=25.9052924791086, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=359, Percent_Identity=25.9052924791086, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=342, Percent_Identity=30.1169590643275, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323187, Length=342, Percent_Identity=30.1169590643275, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323186, Length=342, Percent_Identity=30.1169590643275, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323184, Length=342, Percent_Identity=30.1169590643275, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=339, Percent_Identity=28.3185840707965, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645530, Length=250, Percent_Identity=29.2, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=25.1366120218579, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36535; Mature: 36535

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVAVVFNGGTISMKVDPRIKAAVPSLSGEEIMAMVTGIEEYAEIENYEFSNYPSPYIT
CCEEEEEEECCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PGQMMDLSKYIKDILEREDICGVVVTHGTDSLEETAYLLDLTIKTHKPVIVTGAMRSSSE
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCEEEEEEEEECCCCEEEEECCCCCCC
LGYDGPANLAASICTAISKEAIGKGVLVCLNDELNCASEVTKSNSTALNTFKSPIFGPIG
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHCCCCCCEE
IVDNNQVIFYREKLKKQHIETDRIESNVALIKCVSGIDSSFIDFCLDRAYKGLVIEAMGR
EEECCEEEEEHHHHHHHCCCHHHHCCCHHEEEEHHCCCHHHHHHHHHHHHCCEEEEECCC
GNVPPNMVPGIKRAIDKNIPVVLVSRCYEGRVLDTYGYEGGGKQLRKMGVIFGDSLPGQK
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHEECCCCCCCC
ARIKLALALTKSNYDVEKIRQIFEDGLYKYTTK
EEEEEEEEEECCCCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MKKVAVVFNGGTISMKVDPRIKAAVPSLSGEEIMAMVTGIEEYAEIENYEFSNYPSPYIT
CCEEEEEEECCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PGQMMDLSKYIKDILEREDICGVVVTHGTDSLEETAYLLDLTIKTHKPVIVTGAMRSSSE
CHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCEEEEEEEEECCCCEEEEECCCCCCC
LGYDGPANLAASICTAISKEAIGKGVLVCLNDELNCASEVTKSNSTALNTFKSPIFGPIG
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHCCCCCCEE
IVDNNQVIFYREKLKKQHIETDRIESNVALIKCVSGIDSSFIDFCLDRAYKGLVIEAMGR
EEECCEEEEEHHHHHHHCCCHHHHCCCHHEEEEHHCCCHHHHHHHHHHHHCCEEEEECCC
GNVPPNMVPGIKRAIDKNIPVVLVSRCYEGRVLDTYGYEGGGKQLRKMGVIFGDSLPGQK
CCCCCCCCHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHEECCCCCCCC
ARIKLALALTKSNYDVEKIRQIFEDGLYKYTTK
EEEEEEEEEECCCCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]