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The map label for this gene is licH [H]

Identifier: 152977421

GI number: 152977421

Start: 3807901

End: 3809226

Strand: Reverse

Name: licH [H]

Synonym: Bcer98_3751

Alternate gene names: 152977421

Gene position: 3809226-3807901 (Counterclockwise)

Preceding gene: 152977422

Following gene: 152977420

Centisome position: 93.2

GC content: 38.08

Gene sequence:

>1326_bases
ATGGAAGGAATTAAAATTGCTACAATTGGTGGTGGCTCTAGTTATACACCAGAGTTAATAGAAGGATTTATTAAAAGGTA
TGATGAATTGCCAGTTCGTGAAATTTGGTTAGTTGATATTGAGGCTGGAAAAGAAAAGTTAGAAATTGTTGGTAATTTGG
CGAAACGAATGGTGAAAAAAGCTGGCTTACCAATTGATATTCATCTTACATTAGATCGCCGTGCAGCATTAAAAGATGCA
GACTTTGTTACAACGCAGCTCCGCGTTGGTTTATTAGAAGCGCGCGCAAAGGATGAAGCAATTCCGTTAAAATATGATGT
AATCGGTCAGGAAACAAATGGCCCTGGTGGTCTATTTAAAGCGTTGAGAACGATTCCTGTTATTTTAGATATTTGTAAAG
ATATGGAGGAGCTTTGTCCAAATGCTTGGTTAATCAACTTTACAAATCCAGCAGGCATGGTAACGGAAGCTGTGCTTCGT
TATACAAATATTGAAAAGGTAGTTGGACTATGTAACGTTCCAATCGGCATTCGTATGGGTCTTGCAAAACTTCTTGAAGT
GGATGCAAGCCGTGTACAAGTTGATTTCGCAGGTTTAAATCATATGGTTTACGGATTAGATGTGTATTTAGATGGGGTAA
GTGTGATGGATCGTGTGTTAGAACTTGTAACGGATCCAGAAAACCAAATTACGATGGAAAATATCGCTGCTCTTGATTGG
GAGCCAGATTTTATTCGTGCACTGCGTGCGATTCCATGTCCATACCATCGCTACTATTATAAAACACGTGAAATGTTAGA
AGAAGAAAAACAGGCTTCTTTAGAAAAAGGAACACGTGCAGAAGTGGTGAAACAATTAGAAGCAGATTTATTTGAACTAT
ATAAAGATCCAAACCTAGATATTAAACCACCGCAATTAGAAAAAAGAGGCGGCGCGTATTATAGTGATGCGGCATGTAGT
TTAATTACATCTATTTACAATAATAAAGGTGATATTCAGCCTGTAAATACGAGAAATAACGGTGCAATCGCAAGCTTACC
GCACGATTCTGCTGTTGAAGTAAACTGCATCATTACAAAGGATGGCCCAAAACCAGTGACGGTCGGTGACTTACCAGTTC
CAGTTCGCGGTTTAGTACAGCAAATTAAATCCTTTGAACGTACAACAATTGAAGCAGCTGTTACAGGGGATTATCATAAA
GCACTGCTTGCAATGACAATTAACCCACTTGTACCATCAGATAAATTGGCAAAACAAATTTTAGATGAAATGTTGGAAGC
GCATAAAGAACATCTTCCGCAGTTCTTTAAAAAAATAGAGAAATAA

Upstream 100 bases:

>100_bases
TCATGCACAAGATCATTTAATGAATGCAATTACAGTAAAAGAATTAGCAACAGAATTTATTGATCTATATAAAAAGCTAG
ATGCGAAGGGGGAGTAAAGT

Downstream 100 bases:

>100_bases
AAGCGGTGCTTACTAAGCCCGCTTTTTTTCTTGAATCAGGAGGGGACAGGATGATTCAATTAATTGTAAACGCAGATGAC
TTCGGTCTTTCAGAAGGGAC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MEGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKAGLPIDIHLTLDRRAALKDA
DFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFTNPAGMVTEAVLR
YTNIEKVVGLCNVPIGIRMGLAKLLEVDASRVQVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPENQITMENIAALDW
EPDFIRALRAIPCPYHRYYYKTREMLEEEKQASLEKGTRAEVVKQLEADLFELYKDPNLDIKPPQLEKRGGAYYSDAACS
LITSIYNNKGDIQPVNTRNNGAIASLPHDSAVEVNCIITKDGPKPVTVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHK
ALLAMTINPLVPSDKLAKQILDEMLEAHKEHLPQFFKKIEK

Sequences:

>Translated_441_residues
MEGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKAGLPIDIHLTLDRRAALKDA
DFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFTNPAGMVTEAVLR
YTNIEKVVGLCNVPIGIRMGLAKLLEVDASRVQVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPENQITMENIAALDW
EPDFIRALRAIPCPYHRYYYKTREMLEEEKQASLEKGTRAEVVKQLEADLFELYKDPNLDIKPPQLEKRGGAYYSDAACS
LITSIYNNKGDIQPVNTRNNGAIASLPHDSAVEVNCIITKDGPKPVTVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHK
ALLAMTINPLVPSDKLAKQILDEMLEAHKEHLPQFFKKIEK
>Mature_441_residues
MEGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKKAGLPIDIHLTLDRRAALKDA
DFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFKALRTIPVILDICKDMEELCPNAWLINFTNPAGMVTEAVLR
YTNIEKVVGLCNVPIGIRMGLAKLLEVDASRVQVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPENQITMENIAALDW
EPDFIRALRAIPCPYHRYYYKTREMLEEEKQASLEKGTRAEVVKQLEADLFELYKDPNLDIKPPQLEKRGGAYYSDAACS
LITSIYNNKGDIQPVNTRNNGAIASLPHDSAVEVNCIITKDGPKPVTVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHK
ALLAMTINPLVPSDKLAKQILDEMLEAHKEHLPQFFKKIEK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=437, Percent_Identity=51.7162471395881, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1790560, Length=468, Percent_Identity=28.2051282051282, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 49151; Mature: 49151

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKK
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
AGLPIDIHLTLDRRAALKDADFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFK
CCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
ALRTIPVILDICKDMEELCPNAWLINFTNPAGMVTEAVLRYTNIEKVVGLCNVPIGIRMG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHH
LAKLLEVDASRVQVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPENQITMENIAALDW
HHHHHHHCHHHEEEEHHCCCHHEEEHHHEECCHHHHHHHHHHHCCCCCCEEHHHHEECCC
EPDFIRALRAIPCPYHRYYYKTREMLEEEKQASLEKGTRAEVVKQLEADLFELYKDPNLD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSDAACSLITSIYNNKGDIQPVNTRNNGAIASLPHDSAVEVNCIITK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCEEECCCCCCEEEEEEEEC
DGPKPVTVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHKALLAMTINPLVPSDKLAKQI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHEECCCCCCHHHHHHH
LDEMLEAHKEHLPQFFKKIEK
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGIKIATIGGGSSYTPELIEGFIKRYDELPVREIWLVDIEAGKEKLEIVGNLAKRMVKK
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
AGLPIDIHLTLDRRAALKDADFVTTQLRVGLLEARAKDEAIPLKYDVIGQETNGPGGLFK
CCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
ALRTIPVILDICKDMEELCPNAWLINFTNPAGMVTEAVLRYTNIEKVVGLCNVPIGIRMG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHH
LAKLLEVDASRVQVDFAGLNHMVYGLDVYLDGVSVMDRVLELVTDPENQITMENIAALDW
HHHHHHHCHHHEEEEHHCCCHHEEEHHHEECCHHHHHHHHHHHCCCCCCEEHHHHEECCC
EPDFIRALRAIPCPYHRYYYKTREMLEEEKQASLEKGTRAEVVKQLEADLFELYKDPNLD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
IKPPQLEKRGGAYYSDAACSLITSIYNNKGDIQPVNTRNNGAIASLPHDSAVEVNCIITK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCEEECCCCCCEEEEEEEEC
DGPKPVTVGDLPVPVRGLVQQIKSFERTTIEAAVTGDYHKALLAMTINPLVPSDKLAKQI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHEECCCCCCHHHHHHH
LDEMLEAHKEHLPQFFKKIEK
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]