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The map label for this gene is yhcA [H]

Identifier: 152976951

GI number: 152976951

Start: 3320209

End: 3321948

Strand: Reverse

Name: yhcA [H]

Synonym: Bcer98_3254

Alternate gene names: 152976951

Gene position: 3321948-3320209 (Counterclockwise)

Preceding gene: 152976952

Following gene: 152976947

Centisome position: 81.28

GC content: 36.49

Gene sequence:

>1740_bases
ATGTCCTCATCTTTAATAGTAGGATATGCTATATTTTGTATTATAGTCTTCTTCCTGGTAAATCGTCTTTTTAGAAAGAA
AAAAACAAACGCGGGAGAAGAGAAGGTCCCAGCCGTAAGTAAAATAGAAACGAAGCAACCGGAACAAAAAGCTTCCAATC
TTGTAGAATTAGAAGCAGCTAAGAAAGAAGTACCCAAAAAGCATTTGCCAATAGAAAATGTAAATGTAAAAGCAGTTGTG
ACAGTATTGATTCTTGGCATGTTTGTTTCGATTTTAAACCAAACGATTATTAATGTTGCTTTGCCACCATTAATGAATGA
ATTTAATGTCTCAACTTCAACAGCGCAATGGTTAATTACAGGATTTATGCTTGTAAACGGGATTTTAGTCCCAATCAGTG
CCTTTTTAGTATCGCGCTTTACGTATCGTAATTTATTTATAGCAGCAATGGTATTTTTTACAGTAGGTTCTATTATTTGT
GCTATATCTGGAAACTTTACGATGATGATGATAGGCCGTGTAATTCAAGCAGTTGGTGCAGGTGTTTTAATGCCAATTGG
TATGAATATTTTTATGACACTGTTCCCTCCCCATAAGCGAGGAGCTGCGATGGGATTATTAGGGGTAGCGATGATTTTAG
CACCAGCTATTGGACCGACTGTAACAGGTTGGGTCATTGAAAATTACAGCTGGAACTTAATGTTCTATGTGATGTTTGTG
ATTGGCCTAATCATTACTTTATTATCATTAAAATTCTTTACACTAGCACAGCCAGTATCGAAAGCGAAATTGGATTTATT
TGGTGTAATTAGCTCAAGTATTGGACTTGGTAGCTTATTATATGGATTTAGTGAAGCCGGCAATAATAGTTGGAGCAGTA
CAGAAGTTGTTGTTTCTCTGATCATCGGTACAATTGGTCTTGCATTGTTTATTTGGAGAGAAATGACGACGGATAATAAA
ATGTTGGATTTACATGTATTTAAGTATCCAACATTTACTTTCACATTGTTAATTAACGCAATTGTAACAATGGCGCTATT
TGGAGGTATGTTATTACTTCCTGTCTATCTGCAAAATATTCGTGGTTTTACGCCAATTGAATCCGGTCTACTGCTTCTTC
CAGGATCATTAATTATGGGGATTATGGGACCGATTGCAGGGAAACTATTTGATAAGTATGGCATTCGTCCCTTAGCAATC
ATTGGGTTAGCAATTACAACATATGCAACATATGAATTTACGAAATTAACAATGGAGACACCATATAGTGTAATCATGAG
AGACTACATCATACGTTCAATCGGAATGTCGTTTATTATGATGCCAATTATGACAGCTGGTATGAATGCGTTGCCGATGA
AATTGATTTCGCATGGTACAGCAACACAAAATACGTCAAGACAAGTCGCCGGTTCAATTGGTACAGCGATTTTAATTACA
ATTATGACGCAGCAAACAAACTATCACGTAGCAGATTACGGAAATATGTTAACAACGACAAATCCGATTTTAGTTGATAA
AGTACACGGAATGGGACAAAGCTTAGCAGCTTTAGCTGGTTCCGCACAAGCTGGAGATGTGATGAGTACGCAACTATTAT
ACGGACAAATTTCAAAGCTATCTGCAATTAACGGTATTAATGATGCGTTCTTAATTGCAACCATATTAGCGGGTATTGCC
TGGGTATTATCATTCTTCTTGAAATCAGGTAATAAGCCAAATCGAAAAGCAGGGAATTAA

Upstream 100 bases:

>100_bases
TAGTACCGGTTAAAATTTCTTTAGACCACAGTAAGTCAGTAAATATTGTTCCTGGAATGAATGTGACAGTTCGTATTCAT
AAATAAGGGGGGGAGATAAA

Downstream 100 bases:

>100_bases
TGTCCCTGCTTTTTTATAGTTAATCTGTAACGAGAAGTTGATGAGCAATAACTGTAAACGATTCTGTTATATAGAGAATG
AATTAGGTAGGGACATCAAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MSSSLIVGYAIFCIIVFFLVNRLFRKKKTNAGEEKVPAVSKIETKQPEQKASNLVELEAAKKEVPKKHLPIENVNVKAVV
TVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRNLFIAAMVFFTVGSIIC
AISGNFTMMMIGRVIQAVGAGVLMPIGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTVTGWVIENYSWNLMFYVMFV
IGLIITLLSLKFFTLAQPVSKAKLDLFGVISSSIGLGSLLYGFSEAGNNSWSSTEVVVSLIIGTIGLALFIWREMTTDNK
MLDLHVFKYPTFTFTLLINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPIAGKLFDKYGIRPLAI
IGLAITTYATYEFTKLTMETPYSVIMRDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTATQNTSRQVAGSIGTAILIT
IMTQQTNYHVADYGNMLTTTNPILVDKVHGMGQSLAALAGSAQAGDVMSTQLLYGQISKLSAINGINDAFLIATILAGIA
WVLSFFLKSGNKPNRKAGN

Sequences:

>Translated_579_residues
MSSSLIVGYAIFCIIVFFLVNRLFRKKKTNAGEEKVPAVSKIETKQPEQKASNLVELEAAKKEVPKKHLPIENVNVKAVV
TVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRNLFIAAMVFFTVGSIIC
AISGNFTMMMIGRVIQAVGAGVLMPIGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTVTGWVIENYSWNLMFYVMFV
IGLIITLLSLKFFTLAQPVSKAKLDLFGVISSSIGLGSLLYGFSEAGNNSWSSTEVVVSLIIGTIGLALFIWREMTTDNK
MLDLHVFKYPTFTFTLLINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPIAGKLFDKYGIRPLAI
IGLAITTYATYEFTKLTMETPYSVIMRDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTATQNTSRQVAGSIGTAILIT
IMTQQTNYHVADYGNMLTTTNPILVDKVHGMGQSLAALAGSAQAGDVMSTQLLYGQISKLSAINGINDAFLIATILAGIA
WVLSFFLKSGNKPNRKAGN
>Mature_578_residues
SSSLIVGYAIFCIIVFFLVNRLFRKKKTNAGEEKVPAVSKIETKQPEQKASNLVELEAAKKEVPKKHLPIENVNVKAVVT
VLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRNLFIAAMVFFTVGSIICA
ISGNFTMMMIGRVIQAVGAGVLMPIGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTVTGWVIENYSWNLMFYVMFVI
GLIITLLSLKFFTLAQPVSKAKLDLFGVISSSIGLGSLLYGFSEAGNNSWSSTEVVVSLIIGTIGLALFIWREMTTDNKM
LDLHVFKYPTFTFTLLINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPIAGKLFDKYGIRPLAII
GLAITTYATYEFTKLTMETPYSVIMRDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTATQNTSRQVAGSIGTAILITI
MTQQTNYHVADYGNMLTTTNPILVDKVHGMGQSLAALAGSAQAGDVMSTQLLYGQISKLSAINGINDAFLIATILAGIAW
VLSFFLKSGNKPNRKAGN

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=27.5247524752475, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788710, Length=512, Percent_Identity=26.3671875, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081983, Length=435, Percent_Identity=24.8275862068966, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1790195, Length=339, Percent_Identity=26.2536873156342, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=25.9803921568627, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6322958, Length=303, Percent_Identity=25.7425742574257, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=316, Percent_Identity=22.7848101265823, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=227, Percent_Identity=25.5506607929515, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 62808; Mature: 62677

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLIVGYAIFCIIVFFLVNRLFRKKKTNAGEEKVPAVSKIETKQPEQKASNLVELEAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCCCHHHHHHHHHHHHH
KKEVPKKHLPIENVNVKAVVTVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLIT
HHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
GFMLVNGILVPISAFLVSRFTYRNLFIAAMVFFTVGSIICAISGNFTMMMIGRVIQAVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GVLMPIGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTVTGWVIENYSWNLMFYVMFV
CCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
IGLIITLLSLKFFTLAQPVSKAKLDLFGVISSSIGLGSLLYGFSEAGNNSWSSTEVVVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHH
IIGTIGLALFIWREMTTDNKMLDLHVFKYPTFTFTLLINAIVTMALFGGMLLLPVYLQNI
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGFTPIESGLLLLPGSLIMGIMGPIAGKLFDKYGIRPLAIIGLAITTYATYEFTKLTMET
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEHHC
PYSVIMRDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTATQNTSRQVAGSIGTAILIT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEE
IMTQQTNYHVADYGNMLTTTNPILVDKVHGMGQSLAALAGSAQAGDVMSTQLLYGQISKL
EEECCCCEEECCCCCEECCCCCEEEHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SAINGINDAFLIATILAGIAWVLSFFLKSGNKPNRKAGN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSSLIVGYAIFCIIVFFLVNRLFRKKKTNAGEEKVPAVSKIETKQPEQKASNLVELEAA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCCCHHHHHHHHHHHHH
KKEVPKKHLPIENVNVKAVVTVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLIT
HHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
GFMLVNGILVPISAFLVSRFTYRNLFIAAMVFFTVGSIICAISGNFTMMMIGRVIQAVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GVLMPIGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTVTGWVIENYSWNLMFYVMFV
CCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
IGLIITLLSLKFFTLAQPVSKAKLDLFGVISSSIGLGSLLYGFSEAGNNSWSSTEVVVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHH
IIGTIGLALFIWREMTTDNKMLDLHVFKYPTFTFTLLINAIVTMALFGGMLLLPVYLQNI
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGFTPIESGLLLLPGSLIMGIMGPIAGKLFDKYGIRPLAIIGLAITTYATYEFTKLTMET
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEHHC
PYSVIMRDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTATQNTSRQVAGSIGTAILIT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEE
IMTQQTNYHVADYGNMLTTTNPILVDKVHGMGQSLAALAGSAQAGDVMSTQLLYGQISKL
EEECCCCEEECCCCCEECCCCCEEEHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SAINGINDAFLIATILAGIAWVLSFFLKSGNKPNRKAGN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]