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The map label for this gene is ysgA [H]

Identifier: 152976947

GI number: 152976947

Start: 3317581

End: 3318345

Strand: Reverse

Name: ysgA [H]

Synonym: Bcer98_3250

Alternate gene names: 152976947

Gene position: 3318345-3317581 (Counterclockwise)

Preceding gene: 152976951

Following gene: 152976946

Centisome position: 81.19

GC content: 38.43

Gene sequence:

>765_bases
ATGAAAAATATTGATTCAGTAAAAAATCCGCGTGTGAAGCAGTGGAAAAAGTTACAAACAAAAAAAGAACGCGATAAGAA
AGGACTATTCTTTGTAGAAGGATTCCATTTAGTAGAAGAAGCTTTAAAAGCAGACATTGTTACAGAACTTATCGTTTCAG
ATCAAACGGACCTTCCGAAAGATTGGATCGTTTCTGATGTCGAAATGTATATTGTCCCTGAACCGATTGTAAAGGTGCTT
AGGGAAACAGAAACAACACAAGGTGTATTTGCAGTTTGCGAGAAACAAGAAAAAGAAGTAGAGCTTTCAAATGGGAAATT
TCTTTTGCTCGATGGTCTTCAAGATCCAGGCAATTTAGGGACAATTATTCGCACGGCTGATGCAGCAGGTGTTCATGCTG
TTGTACTTGGAGAAGGATGTGTAGACGCTTATAATAGTAAAGTGCTCCGCTCTACACAAGGCTCTATTTTTCATTTGCCA
GTTGTCAAAGGGAATTTAGAAGAATGGATAGACAAGTTAAAAACACATAACGTTCCTGTATACGGAACAGCGCTAGAAAA
TGGTGTTCCATATGGCGAAATAACACCAACAGGAAGTTTCGCATTAATTGTAGGAAATGAAGGAAATGGTGTGCACCAAG
ATATTCTTGCAAAATGTGATCAAAACTTATATATTCCGATTTATGGCGGGGCCGAGTCGCTAAATGTAGCAGTAGCTGCT
GGAATTTTAACGTATTATTTACAAAGCCCGGTTGCGAATCGTTAA

Upstream 100 bases:

>100_bases
TTTCGGAATTGAAACTGCCATTAGTTTTTGTTCTTTTTTATGTAAATATGTATGAAAAATAGGCTATAATGAGAAGACAA
GAAGAGAGGGAATGCATAGA

Downstream 100 bases:

>100_bases
AAAACTTCTTATAATAAGTTGAACATATAATAAAAAGAATAGAATGAAAAACATTGATAGAGAAGAGTAACTTTCAAAAT
CGCCATATAGGGAGAGAATG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNIDSVKNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKADIVTELIVSDQTDLPKDWIVSDVEMYIVPEPIVKVL
RETETTQGVFAVCEKQEKEVELSNGKFLLLDGLQDPGNLGTIIRTADAAGVHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
VVKGNLEEWIDKLKTHNVPVYGTALENGVPYGEITPTGSFALIVGNEGNGVHQDILAKCDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANR

Sequences:

>Translated_254_residues
MKNIDSVKNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKADIVTELIVSDQTDLPKDWIVSDVEMYIVPEPIVKVL
RETETTQGVFAVCEKQEKEVELSNGKFLLLDGLQDPGNLGTIIRTADAAGVHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
VVKGNLEEWIDKLKTHNVPVYGTALENGVPYGEITPTGSFALIVGNEGNGVHQDILAKCDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANR
>Mature_254_residues
MKNIDSVKNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKADIVTELIVSDQTDLPKDWIVSDVEMYIVPEPIVKVL
RETETTQGVFAVCEKQEKEVELSNGKFLLLDGLQDPGNLGTIIRTADAAGVHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
VVKGNLEEWIDKLKTHNVPVYGTALENGVPYGEITPTGSFALIVGNEGNGVHQDILAKCDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=291, Percent_Identity=27.1477663230241, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790623, Length=140, Percent_Identity=36.4285714285714, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=30.2013422818792, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666840, Length=272, Percent_Identity=28.3088235294118, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27890; Mature: 27890

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIDSVKNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKADIVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DWIVSDVEMYIVPEPIVKVLRETETTQGVFAVCEKQEKEVELSNGKFLLLDGLQDPGNLG
HHHHCCEEEEEECHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCC
TIIRTADAAGVHAVVLGEGCVDAYNSKVLRSTQGSIFHLPVVKGNLEEWIDKLKTHNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCE
YGTALENGVPYGEITPTGSFALIVGNEGNGVHQDILAKCDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANR
HHHHHHHHCCCCCC
>Mature Secondary Structure
MKNIDSVKNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKADIVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DWIVSDVEMYIVPEPIVKVLRETETTQGVFAVCEKQEKEVELSNGKFLLLDGLQDPGNLG
HHHHCCEEEEEECHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCC
TIIRTADAAGVHAVVLGEGCVDAYNSKVLRSTQGSIFHLPVVKGNLEEWIDKLKTHNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCE
YGTALENGVPYGEITPTGSFALIVGNEGNGVHQDILAKCDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANR
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]