| Definition | Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome. |
|---|---|
| Accession | NC_009674 |
| Length | 4,087,024 |
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The map label for this gene is polX [H]
Identifier: 152976938
GI number: 152976938
Start: 3307207
End: 3308925
Strand: Reverse
Name: polX [H]
Synonym: Bcer98_3241
Alternate gene names: 152976938
Gene position: 3308925-3307207 (Counterclockwise)
Preceding gene: 152976939
Following gene: 152976937
Centisome position: 80.96
GC content: 38.86
Gene sequence:
>1719_bases ATGAAAGTAAATAAAAAACAAGTGATTAAATTATTAGAAACAATCGCCCTTTTTATGGAATTAAAAGGCGAGAATCCATT TAAAATATCCGCATTTCGTAAAGCGGCAGCAGCATTAGAAAGTGATGATCGCAGCCTTTCTGAAATTGAAGATTTCACAA AGATTCCAGGCATCGGAAAAGGAACTGCAGCAGTTATTCAAGAATATATGGAAGCTGGAACGTCCGAAGTATTGCAGGAG CTTGAAAAAGAAGTGCCTAGTAGCTTATTGCCATTATTAAAACTTCCAGGACTCGGTGGTAAGAAAGTGGCGAAACTTTA CAAAGAACTGGGCGTTGTTGACATGGAAACATTAAAGCAAGCTTGTGAAGAAAAGAAAGTACAGGAGCTTGCTGGGTTTG GGAAGAAGACGGAAGAGAAAATATTAGAAGCAATTGCGCAAGCTGGTTCTCGTCCTGAACGTTTACCGATTGCAATGGTA CTTCCTATTGCCGGGGAAATAGAAGAAAAATTGTCGAACATTGCAGAAGTCATTCGTTTTTCGCGCGCGGGTAGTTTGCG CCGCGTTCGGGAGACTGTGAAAGATTTAGACTTCATTATTGCAACGACAGAACCAGCAGCAGTACGTGAACATTTGCTGC AATTTGATAATATGATTGAAGTGATTGCAAGCGGTGATACAAAAGTATCTGTTCGCCTGCAATATGAATATGATATTTCA ATTGATTTTCGCCTTGTGAAACCTGAGGAATTTATTACAACGCTTCATCATTTTACAGGTTCAAAAGATCATAACGTAAG AATGCGTCAGATTGCAAAAGATAACGGTGAAAAGATCAGTGAGTATGGTGTGGAAAATTTGGAGACAGGTGAAGTGAAAA CATTCGAAACAGAAGAAGAGTTTTTTGCTCATTTCAACCTTCCGTTTATTCCGCCAGAAGTGCGCGAGGATGGAAAAGAG ATTGAACTCATTAAGGAATATCCAAACTTAATTCAGTTCTCTGATATACAAGGTGATCTCCATATGCATACAACATGGAG TGATGGTGCTTTTTCCATTGAAGAGATGGTACAAGCATGCCGCGCGCGTGGGTATAAATTTATGGCAATTACGGATCACT CTCAATATTTGAAAGTAGCGAATGGCTTAACGAAAGAGCGACTTCGTGAACAAGCAAAAGAAATTGAGCGCATCAATGCG AAATACCCAGATATCACAATTTTACGTGGGATTGAAATGGACATACTGCCAGATGCGACATTAGATTTTGATGATGAGGT ATTAGCGGAGCTTGATTATGTAATTGGTGCGATTCATTCCAGTTTCTCACAAGATCGTGAAACAATTATGAAACGTTTGC GCACCGCAATTGAAAATAAGCATGTAACAATGATTGCTCATCCAACAGGACGTCTTCTTGGACGCCGTGAGGGCTATGAT GTAGATACAGATTTATTAATTGAGCTCGCAAAAGAAACAAATACTGTTTTAGAATTAAATGCAAATCCGAACCGTCTAGA TTTAAGTGCGAAATTATTAAAACAGGCACAAGATGCTGGTGTGAAAATCGCAATTAATACAGATGCTCATACACTTGAGA TGTTAGAGGATATGGAAACAGGTGTAGCGGTAGCGCGCAAAGGTTGGATTCAAAAAGATACTGTGATTAACACATGGGAT ATTGAACGTTTATTAGATTTTATTAAACGTAATAAGTAA
Upstream 100 bases:
>100_bases TTCTGAAAAGGTGAAGGAACTATGGCAGACAGGTAGCAGAGTATAACTTCTCTTTTTCGGAAAGAGAAGTTTTTTTCTAT GAAGGAAAGGCGGGGAACGT
Downstream 100 bases:
>100_bases CACAAGGGGGACAGGCAACTTATGTTAGAACGAACGTTGCGTGTATTAGAATATGACAAAGTAAAAGAACAGTTACTTGA GCATACAGCTTCTTCGCTTG
Product: hypothetical protein
Products: NA
Alternate protein names: DNA polymerase type-X; 3'-5' exodeoxyribonuclease; 3'-5' exonuclease [H]
Number of amino acids: Translated: 572; Mature: 572
Protein sequence:
>572_residues MKVNKKQVIKLLETIALFMELKGENPFKISAFRKAAAALESDDRSLSEIEDFTKIPGIGKGTAAVIQEYMEAGTSEVLQE LEKEVPSSLLPLLKLPGLGGKKVAKLYKELGVVDMETLKQACEEKKVQELAGFGKKTEEKILEAIAQAGSRPERLPIAMV LPIAGEIEEKLSNIAEVIRFSRAGSLRRVRETVKDLDFIIATTEPAAVREHLLQFDNMIEVIASGDTKVSVRLQYEYDIS IDFRLVKPEEFITTLHHFTGSKDHNVRMRQIAKDNGEKISEYGVENLETGEVKTFETEEEFFAHFNLPFIPPEVREDGKE IELIKEYPNLIQFSDIQGDLHMHTTWSDGAFSIEEMVQACRARGYKFMAITDHSQYLKVANGLTKERLREQAKEIERINA KYPDITILRGIEMDILPDATLDFDDEVLAELDYVIGAIHSSFSQDRETIMKRLRTAIENKHVTMIAHPTGRLLGRREGYD VDTDLLIELAKETNTVLELNANPNRLDLSAKLLKQAQDAGVKIAINTDAHTLEMLEDMETGVAVARKGWIQKDTVINTWD IERLLDFIKRNK
Sequences:
>Translated_572_residues MKVNKKQVIKLLETIALFMELKGENPFKISAFRKAAAALESDDRSLSEIEDFTKIPGIGKGTAAVIQEYMEAGTSEVLQE LEKEVPSSLLPLLKLPGLGGKKVAKLYKELGVVDMETLKQACEEKKVQELAGFGKKTEEKILEAIAQAGSRPERLPIAMV LPIAGEIEEKLSNIAEVIRFSRAGSLRRVRETVKDLDFIIATTEPAAVREHLLQFDNMIEVIASGDTKVSVRLQYEYDIS IDFRLVKPEEFITTLHHFTGSKDHNVRMRQIAKDNGEKISEYGVENLETGEVKTFETEEEFFAHFNLPFIPPEVREDGKE IELIKEYPNLIQFSDIQGDLHMHTTWSDGAFSIEEMVQACRARGYKFMAITDHSQYLKVANGLTKERLREQAKEIERINA KYPDITILRGIEMDILPDATLDFDDEVLAELDYVIGAIHSSFSQDRETIMKRLRTAIENKHVTMIAHPTGRLLGRREGYD VDTDLLIELAKETNTVLELNANPNRLDLSAKLLKQAQDAGVKIAINTDAHTLEMLEDMETGVAVARKGWIQKDTVINTWD IERLLDFIKRNK >Mature_572_residues MKVNKKQVIKLLETIALFMELKGENPFKISAFRKAAAALESDDRSLSEIEDFTKIPGIGKGTAAVIQEYMEAGTSEVLQE LEKEVPSSLLPLLKLPGLGGKKVAKLYKELGVVDMETLKQACEEKKVQELAGFGKKTEEKILEAIAQAGSRPERLPIAMV LPIAGEIEEKLSNIAEVIRFSRAGSLRRVRETVKDLDFIIATTEPAAVREHLLQFDNMIEVIASGDTKVSVRLQYEYDIS IDFRLVKPEEFITTLHHFTGSKDHNVRMRQIAKDNGEKISEYGVENLETGEVKTFETEEEFFAHFNLPFIPPEVREDGKE IELIKEYPNLIQFSDIQGDLHMHTTWSDGAFSIEEMVQACRARGYKFMAITDHSQYLKVANGLTKERLREQAKEIERINA KYPDITILRGIEMDILPDATLDFDDEVLAELDYVIGAIHSSFSQDRETIMKRLRTAIENKHVTMIAHPTGRLLGRREGYD VDTDLLIELAKETNTVLELNANPNRLDLSAKLLKQAQDAGVKIAINTDAHTLEMLEDMETGVAVARKGWIQKDTVINTWD IERLLDFIKRNK
Specific function: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a sing
COG id: COG1796
COG function: function code L; DNA polymerase IV (family X)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the PHP family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002054 - InterPro: IPR010996 - InterPro: IPR003583 - InterPro: IPR004013 - InterPro: IPR003141 - InterPro: IPR016195 - InterPro: IPR022311 [H]
Pfam domain/function: PF02811 PHP [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 64700; Mature: 64700
Theoretical pI: Translated: 4.93; Mature: 4.93
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKVNKKQVIKLLETIALFMELKGENPFKISAFRKAAAALESDDRSLSEIEDFTKIPGIGK CCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC GTAAVIQEYMEAGTSEVLQELEKEVPSSLLPLLKLPGLGGKKVAKLYKELGVVDMETLKQ CHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHH ACEEKKVQELAGFGKKTEEKILEAIAQAGSRPERLPIAMVLPIAGEIEEKLSNIAEVIRF HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH SRAGSLRRVRETVKDLDFIIATTEPAAVREHLLQFDNMIEVIASGDTKVSVRLQYEYDIS HCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEE IDFRLVKPEEFITTLHHFTGSKDHNVRMRQIAKDNGEKISEYGVENLETGEVKTFETEEE EEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECHHH FFAHFNLPFIPPEVREDGKEIELIKEYPNLIQFSDIQGDLHMHTTWSDGAFSIEEMVQAC HHHHCCCCCCCHHHHCCCCHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHH RARGYKFMAITDHSQYLKVANGLTKERLREQAKEIERINAKYPDITILRGIEMDILPDAT HHCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCC LDFDDEVLAELDYVIGAIHSSFSQDRETIMKRLRTAIENKHVTMIAHPTGRLLGRREGYD CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCC VDTDLLIELAKETNTVLELNANPNRLDLSAKLLKQAQDAGVKIAINTDAHTLEMLEDMET CCHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH GVAVARKGWIQKDTVINTWDIERLLDFIKRNK HHHHHHCCCCCCCCEECHHHHHHHHHHHHCCC >Mature Secondary Structure MKVNKKQVIKLLETIALFMELKGENPFKISAFRKAAAALESDDRSLSEIEDFTKIPGIGK CCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC GTAAVIQEYMEAGTSEVLQELEKEVPSSLLPLLKLPGLGGKKVAKLYKELGVVDMETLKQ CHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHH ACEEKKVQELAGFGKKTEEKILEAIAQAGSRPERLPIAMVLPIAGEIEEKLSNIAEVIRF HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH SRAGSLRRVRETVKDLDFIIATTEPAAVREHLLQFDNMIEVIASGDTKVSVRLQYEYDIS HCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEE IDFRLVKPEEFITTLHHFTGSKDHNVRMRQIAKDNGEKISEYGVENLETGEVKTFETEEE EEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECHHH FFAHFNLPFIPPEVREDGKEIELIKEYPNLIQFSDIQGDLHMHTTWSDGAFSIEEMVQAC HHHHCCCCCCCHHHHCCCCHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHH RARGYKFMAITDHSQYLKVANGLTKERLREQAKEIERINAKYPDITILRGIEMDILPDAT HHCCEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCC LDFDDEVLAELDYVIGAIHSSFSQDRETIMKRLRTAIENKHVTMIAHPTGRLLGRREGYD CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCC VDTDLLIELAKETNTVLELNANPNRLDLSAKLLKQAQDAGVKIAINTDAHTLEMLEDMET CCHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH GVAVARKGWIQKDTVINTWDIERLLDFIKRNK HHHHHHCCCCCCCCEECHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8969504; 9384377 [H]