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The map label for this gene is tgt [H]

Identifier: 152976835

GI number: 152976835

Start: 3182624

End: 3183763

Strand: Reverse

Name: tgt [H]

Synonym: Bcer98_3134

Alternate gene names: 152976835

Gene position: 3183763-3182624 (Counterclockwise)

Preceding gene: 152976836

Following gene: 152976834

Centisome position: 77.9

GC content: 40.53

Gene sequence:

>1140_bases
ATGACAGCAATTCGTTACGAATTTATTAAGAAATGTAAACAAACGGGCGCACGTTTAGGTCGTGTGCATACGCCACATGG
ATCTTTTGATACACCGACCTTTATGCCAGTTGGTACATTAGCAACAGTGAAAACAATGTCACCAGAAGAATTAAAAGCGA
TGGATTCTGGCATTATTTTAAGTAATACGTATCATCTATGGCTGCGTCCAGGTCATGAAATTGTACGTGAAGCAGGTGGA
CTGCATAAATTTATGAACTGGGATCGTGCTATTTTAACGGATTCTGGTGGATTCCAAGTATTTAGTTTAAGTGACTTCCG
TCGTATTGAAGAAGAAGGTGTGTATTTCCGCAATCATTTAAATGGCGACAAGCTATTTTTATCGCCAGAAAAAGCGATGG
AAATTCAAAATGCTTTAGGTTCAGATATTATGATGGCATTTGATGAATGCCCACCATTCCCGGCTACTTTTGAATATATG
AAAAAGTCTGTAGAGCGTACAAGCCGTTGGGCAGAGCGTTGTTTAAACGCGCATCAGCGCCCGGAAGATCAAGGGTTATT
CGGTATTGTACAGGGGGGGGAGTTTGAAGAGCTTCGTCGCCAAAGTGCTAAAGACCTTGTTTCAATGGACTTCCCTGGTT
ACGCTGTAGGTGGTTTATCAGTAGGGGAGCCGAAAGATATCATGAATCGTGTCCTTGAGTTTACAACACCGCTTCTTCCA
GAAAATAAACCTCGTTATTTAATGGGAGTCGGTTCTCCTGATTCATTAATTGATGGTGCAATTCGCGGTATTGACATGTT
TGACTGTGTACTGCCAACTCGCATTGCTCGAAATGGTACTTGTATGACAAGTCAAGGTCGTCTCGTTGTGAAAAATGCGA
AATTTGCAAGAGACTTTGGTCCACTTGATCCAAATTGTGATTGTTACACATGTAAAAATTATTCTCGTGCATACATTCGT
CACTTAATGAAATGTGATGAAACGTTCGGAATTCGTTTAACATCTTATCACAATCTGCATTTTCTGTTAAACTTAATGGA
GCAGGTGAGACAAGCCATTCGTGAAGATCGTCTTGGCGATTTCCGCGAAGAGTTCTTTGAACAGTATGGCTTTAATAAAC
CGAATGCTAAAAACTTTTAA

Upstream 100 bases:

>100_bases
TGAAGCAGTGAAAGAAAAATACCGCTTCTTTAGTTTTGGTGATGCGATGTTCCTTGCATCTCATGCTAAAGAGAGAATGA
AATAAAGAAGGAGTAAATGT

Downstream 100 bases:

>100_bases
TACATAATATTAAAGGAGGAACAAAAATGAGTCCTGGTATGATGAATATTGTCATGATTGTTGCGATGTTTGCGATTTTC
TATTTCTTATTAATTCGCCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTAIRYEFIKKCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIVREAGG
LHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVYFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYM
KKSVERTSRWAERCLNAHQRPEDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
ENKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSQGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIR
HLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF

Sequences:

>Translated_379_residues
MTAIRYEFIKKCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIVREAGG
LHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVYFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYM
KKSVERTSRWAERCLNAHQRPEDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
ENKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSQGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIR
HLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF
>Mature_378_residues
TAIRYEFIKKCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIVREAGGL
HKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVYFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMK
KSVERTSRWAERCLNAHQRPEDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLPE
NKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSQGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIRH
LMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=45.6043956043956, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=26.7123287671233, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=55.524861878453, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=49.1525423728814, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146502, Length=247, Percent_Identity=28.3400809716599, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=44.2048517520216, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI21355311, Length=263, Percent_Identity=25.4752851711027, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43266; Mature: 43135

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIRYEFIKKCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL
CCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHCCEEE
SNTYHLWLRPGHEIVREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVYFRNHL
ECCEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCCCEEEEECHHHHHHHHCCCEEEECC
NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLNAHQR
CCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
ENKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSQGRLVVKNAKFARDFG
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHCC
PLDPNCDCYTCKNYSRAYIRHLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGD
CCCCCCCEEECCCHHHHHHHHHHHCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
FREEFFEQYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAIRYEFIKKCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL
CCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHCCEEE
SNTYHLWLRPGHEIVREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVYFRNHL
ECCEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCCCEEEEECHHHHHHHHCCCEEEECC
NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLNAHQR
CCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
ENKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSQGRLVVKNAKFARDFG
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHCC
PLDPNCDCYTCKNYSRAYIRHLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGD
CCCCCCCEEECCCHHHHHHHHHHHCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
FREEFFEQYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA