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The map label for this gene is queA [H]

Identifier: 152976836

GI number: 152976836

Start: 3183779

End: 3184831

Strand: Reverse

Name: queA [H]

Synonym: Bcer98_3135

Alternate gene names: 152976836

Gene position: 3184831-3183779 (Counterclockwise)

Preceding gene: 152976837

Following gene: 152976835

Centisome position: 77.93

GC content: 37.61

Gene sequence:

>1053_bases
ATGGATATTAATCTGTTTGATTTTCATTTACCAGAAGAACTTATTGCGCAAACGCCGCTTGAAAAGCGTGAAACATCAAG
GTTAATGGTGTTAGATCGTGAAACAGGTGATATCGAGCATAAGCATTTTAGCGATATTTTAGCGTATTTACATGAGGGAG
ATTGTTTAGTTTTAAATGAAACCAAAGTTATGCCTGCTCGTTTGCATGGTGTAAAAGAAGATACAGGTGCACATATTGAG
GTGCTTCTTTTAAAACAAGAAGAGGGAGATACGTGGGAAACACTTGTAAAACCGGCGAAACGTGTGAAAGAAGGAACTGT
TATTTCTTTTGGCGAAGGAAAACTAAAAGCAACATGTGTAGGAACTGCTGATCAAGGCGGTCGTCAGCTTGAATTCTCTT
ATGACGGTATTTTTTATGAGATTTTAGATGAACTGGGAGAAATGCCACTTCCCCCTTATATTAAAGAAACATTAGAAGAC
CGCGATCGTTACCAAACTGTATATGCGAAGGAAATTGGTTCTGCAGCAGCACCAACAGCGGGTCTGCATTTTACAGAAGA
GTTGCTGGAGAAATTGCAACAAAAAGGTGTGAAATTAGCATTCATTACACTTCATGTTGGTCTTGGAACATTTAGACCAG
TTTCGACTGATAAAATTGAAGATCATCATATGCATGCTGAGTATTATCATATGTCGCAGGAAACGGCTGACTTATTAAAT
GACGTGAAAGTAAATGGTGGCCGTATTATTACAGTTGGTACGACATCAACACGTACACTTGAAACAATTGCTACAGAGCA
TGATGGTAAACTTTGCGGTGCATCTGGATGGACGGATATCTTTATGTATCCAGGTTATGAATTTAAAGCAATTGATGGTT
TAATTACAAATTTCCATTTACCAAAATCAACATTAATTATGCTTGTGAGTGCATTTGCAGGTAGAGAAAATGTACTTCAT
GCCTATAATGAAGCAGTGAAAGAAAAATACCGCTTCTTTAGTTTTGGTGATGCGATGTTCCTTGCATCTCATGCTAAAGA
GAGAATGAAATAA

Upstream 100 bases:

>100_bases
CTTTTATTTTCCGATCATTACATGTATTGTGTTAAGTATTGTATTATCTTTTATTATGTATATAATAAATCGACTTAAAT
AGAAATAGGTGGATATAGTT

Downstream 100 bases:

>100_bases
AGAAGGAGTAAATGTATGACAGCAATTCGTTACGAATTTATTAAGAAATGTAAACAAACGGGCGCACGTTTAGGTCGTGT
GCATACGCCACATGGATCTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDINLFDFHLPEELIAQTPLEKRETSRLMVLDRETGDIEHKHFSDILAYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDTWETLVKPAKRVKEGTVISFGEGKLKATCVGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLQQKGVKLAFITLHVGLGTFRPVSTDKIEDHHMHAEYYHMSQETADLLN
DVKVNGGRIITVGTTSTRTLETIATEHDGKLCGASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFAGRENVLH
AYNEAVKEKYRFFSFGDAMFLASHAKERMK

Sequences:

>Translated_350_residues
MDINLFDFHLPEELIAQTPLEKRETSRLMVLDRETGDIEHKHFSDILAYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDTWETLVKPAKRVKEGTVISFGEGKLKATCVGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLQQKGVKLAFITLHVGLGTFRPVSTDKIEDHHMHAEYYHMSQETADLLN
DVKVNGGRIITVGTTSTRTLETIATEHDGKLCGASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFAGRENVLH
AYNEAVKEKYRFFSFGDAMFLASHAKERMK
>Mature_350_residues
MDINLFDFHLPEELIAQTPLEKRETSRLMVLDRETGDIEHKHFSDILAYLHEGDCLVLNETKVMPARLHGVKEDTGAHIE
VLLLKQEEGDTWETLVKPAKRVKEGTVISFGEGKLKATCVGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLED
RDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLQQKGVKLAFITLHVGLGTFRPVSTDKIEDHHMHAEYYHMSQETADLLN
DVKVNGGRIITVGTTSTRTLETIATEHDGKLCGASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFAGRENVLH
AYNEAVKEKYRFFSFGDAMFLASHAKERMK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=48.8439306358381, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39428; Mature: 39428

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINLFDFHLPEELIAQTPLEKRETSRLMVLDRETGDIEHKHFSDILAYLHEGDCLVLNE
CCCCEEECCCCHHHHHCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
TKVMPARLHGVKEDTGAHIEVLLLKQEEGDTWETLVKPAKRVKEGTVISFGEGKLKATCV
CCCCHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
ECCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLQQKGVKLAFITLHVGLGTFRPVSTDKIEDHHMHAEYYHMSQETADLLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
DVKVNGGRIITVGTTSTRTLETIATEHDGKLCGASGWTDIFMYPGYEFKAIDGLITNFHL
HCEECCCEEEEECCCCCHHHHHHHHCCCCCEECCCCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFAGRENVLHAYNEAVKEKYRFFSFGDAMFLASHAKERMK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDINLFDFHLPEELIAQTPLEKRETSRLMVLDRETGDIEHKHFSDILAYLHEGDCLVLNE
CCCCEEECCCCHHHHHCCCCCHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
TKVMPARLHGVKEDTGAHIEVLLLKQEEGDTWETLVKPAKRVKEGTVISFGEGKLKATCV
CCCCHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
GTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAAAPTA
ECCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GLHFTEELLEKLQQKGVKLAFITLHVGLGTFRPVSTDKIEDHHMHAEYYHMSQETADLLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
DVKVNGGRIITVGTTSTRTLETIATEHDGKLCGASGWTDIFMYPGYEFKAIDGLITNFHL
HCEECCCEEEEECCCCCHHHHHHHHCCCCCEECCCCCCEEEEECCCCEEHHHHHHHCCCC
PKSTLIMLVSAFAGRENVLHAYNEAVKEKYRFFSFGDAMFLASHAKERMK
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA