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The map label for this gene is aspS [H]

Identifier: 152976820

GI number: 152976820

Start: 3162649

End: 3164418

Strand: Reverse

Name: aspS [H]

Synonym: Bcer98_3118

Alternate gene names: 152976820

Gene position: 3164418-3162649 (Counterclockwise)

Preceding gene: 152976821

Following gene: 152976819

Centisome position: 77.43

GC content: 40.79

Gene sequence:

>1770_bases
ATGGCTGAAAGAACACATGCATGTGGAAGAGTAACAGTAGAAGCAATTGGACAAACCGTTCAATTAAAAGGTTGGGTACA
AAAGCGCCGCGATTTAGGTGGGCTAATCTTTATCGACTTACGTGACCGTACAGGTATTGTACAAGTTGTCTTTAGTCCAG
AAACATCAAAAGAAGCGTTAGAAGTAGCAGAAACAATTCGTGGTGAATACGTATTACATGTAGAAGGTACAGTTGTTGCG
CGCGGAGCTGGTGCAATTAATGAAAATATGGCAACTGGCCAAATTGAAGTGCAAGCGACGAAAGTAACGGTCTTAAATGC
AGCGAAAACAACTCCGATTATTATTGCAGATGATACAGATGCATCAGAAGATGTTCGTTTAAAATATCGTTATTTAGATT
TGCGTCGTCCTGTTATGTACAATACATTCAAAATGCGTCACGATGTAACGAAAACAATTCGCAATTTCTTAGATACAGAA
GAATTTTTAGAGGTGGAAACACCAATTTTAACAAAGAGTACACCAGAAGGTGCTCGAGACTATTTAGTACCAAGTCGTGT
ACATGACGGTGAGTTTTATGCATTACCACAATCTCCGCAACTATTTAAACAGCTTCTTATGGTCGGTGGATTTGAGCGCT
ATTATCAAGTAGCACGTTGTTTCCGTGATGAAGATTTACGTGCGGATCGTCAACCAGAATTTACACAAATCGATATCGAA
GCTTCCTTCTTAACGCAAGAAGAAATTTTAGACATGATGGAACGCATGATGACGAAAGTGATGAAGGATGTAAAAGGGGT
AGAAATTAGCGTACCATTCCCTCGTATGACATATGCTGATGCGATGGCTCGCTATGGCTCTGATAAACCAGATACACGCT
TTGAAATGGAACTAACGGATTTATCTGAATTTGCAGCGGGCTGCGGTTTTAAAGTATTTACAGGTGCTGTAGAAAATGGT
GGACAAGTAAAAGCGATTAATGCAAAAGGTGCAGCAAGCAAATATTCTCGTAAAGATATCGATGCATTAACTGAATTTGT
AAAAGTATACGGTGCAAAAGGTTTAGCTTGGTTAAAAGTGGAAGAAGATGGCTTAAAAGGACCGATTGCGAAGTTCTTTA
ATGAGGAAGAAGCAAATGTAATAATGACTACATTAGAAGCTTCTGCGGGCGATTTATTACTATTCGTAGCTGATAAGAAA
AGTGTTGTGGCAGATAGCTTAGGTGCACTTCGTTTACGCTTAGGTAAAGAGCTGGAACTAATTGACGAAAATAAATTCAA
TTTCTTATGGGTAACAGATTGGCCGCTTCTTGAATATGATGAGGAAGCAAATCGTTACTTTGCGGCGCACCATCCATTCA
CAATGCCGTTCCGTGAAGATGTTGAGCTATTAGAAACAGCGCCTGAAAAAGCGCGTGCACAAGCGTACGACCTTGTATTA
AATGGTTATGAACTTGGCGGTGGATCCCTTCGTATTTACGAGCGTGATGTACAAGAAAAAATGTTCAAAGCACTTGGATT
CACACAAGAAGAGGCGCGAGAGCAGTTCGGTTTCCTATTAGAAGCGTTTGAATATGGTACACCACCACATGGCGGAATCG
CATTAGGTTTAGACCGTCTAGTGATGTTACTTGCTGGTCGTACAAACCTTCGCGATACAATCGCGTTCCCAAAAACAGCA
AGTGCAAGCTGCTTATTAACAGATGCGCCAAGCCCGGTTGCTGGAGCGCAGCTGGAAGAGTTACATTTGAAATTGAATGT
AAAAGAATAG

Upstream 100 bases:

>100_bases
GATTAATTTGAAGGATATGGCGACAGGCGAGCAAGAAGAAGTAGCATTAGATGTATTTGCTTCATATATCGCAGAGAAAT
TAATATAGGGGGAACTTGTA

Downstream 100 bases:

>100_bases
AAATAAGAATGAAGCTAGATGGGGTATACTAACTCCGTCTAGCTTTTTTAGAGTAAACAAAATTTTGATGGCATTCGCAT
GTTTGTTAGATGGCCAGTCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MAERTHACGRVTVEAIGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFSPETSKEALEVAETIRGEYVLHVEGTVVA
RGAGAINENMATGQIEVQATKVTVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMYNTFKMRHDVTKTIRNFLDTE
EFLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
ASFLTQEEILDMMERMMTKVMKDVKGVEISVPFPRMTYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTGAVENG
GQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFNEEEANVIMTTLEASAGDLLLFVADKK
SVVADSLGALRLRLGKELELIDENKFNFLWVTDWPLLEYDEEANRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
NGYELGGGSLRIYERDVQEKMFKALGFTQEEAREQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTA
SASCLLTDAPSPVAGAQLEELHLKLNVKE

Sequences:

>Translated_589_residues
MAERTHACGRVTVEAIGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFSPETSKEALEVAETIRGEYVLHVEGTVVA
RGAGAINENMATGQIEVQATKVTVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMYNTFKMRHDVTKTIRNFLDTE
EFLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
ASFLTQEEILDMMERMMTKVMKDVKGVEISVPFPRMTYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTGAVENG
GQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFNEEEANVIMTTLEASAGDLLLFVADKK
SVVADSLGALRLRLGKELELIDENKFNFLWVTDWPLLEYDEEANRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
NGYELGGGSLRIYERDVQEKMFKALGFTQEEAREQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTA
SASCLLTDAPSPVAGAQLEELHLKLNVKE
>Mature_588_residues
AERTHACGRVTVEAIGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFSPETSKEALEVAETIRGEYVLHVEGTVVAR
GAGAINENMATGQIEVQATKVTVLNAAKTTPIIIADDTDASEDVRLKYRYLDLRRPVMYNTFKMRHDVTKTIRNFLDTEE
FLEVETPILTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIEA
SFLTQEEILDMMERMMTKVMKDVKGVEISVPFPRMTYADAMARYGSDKPDTRFEMELTDLSEFAAGCGFKVFTGAVENGG
QVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKVEEDGLKGPIAKFFNEEEANVIMTTLEASAGDLLLFVADKKS
VVADSLGALRLRLGKELELIDENKFNFLWVTDWPLLEYDEEANRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVLN
GYELGGGSLRIYERDVQEKMFKALGFTQEEAREQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKTAS
ASCLLTDAPSPVAGAQLEELHLKLNVKE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=593, Percent_Identity=41.3153456998314, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI45439306, Length=310, Percent_Identity=26.1290322580645, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI194272210, Length=308, Percent_Identity=25.6493506493506, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI5031815, Length=308, Percent_Identity=25.6493506493506, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI4758762, Length=292, Percent_Identity=27.0547945205479, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=53.5714285714286, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=305, Percent_Identity=25.9016393442623, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790571, Length=308, Percent_Identity=25.3246753246753, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=613, Percent_Identity=34.257748776509, Blast_Score=331, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17551876, Length=308, Percent_Identity=25.974025974026, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=190, Percent_Identity=27.8947368421053, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=190, Percent_Identity=27.8947368421053, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=190, Percent_Identity=27.8947368421053, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=633, Percent_Identity=35.2290679304897, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6323011, Length=265, Percent_Identity=27.5471698113208, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=26.4808362369338, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=216, Percent_Identity=28.2407407407407, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324256, Length=136, Percent_Identity=27.2058823529412, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584738, Length=607, Percent_Identity=40.5271828665568, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=27.6515151515151, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24640849, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24640851, Length=260, Percent_Identity=25.7692307692308, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921528, Length=281, Percent_Identity=25.2669039145907, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66066; Mature: 65935

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERTHACGRVTVEAIGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFSPETSKEAL
CCCCCCCCCCEEHHHCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHH
EVAETIRGEYVLHVEGTVVARGAGAINENMATGQIEVQATKVTVLNAAKTTPIIIADDTD
HHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCC
ASEDVRLKYRYLDLRRPVMYNTFKMRHDVTKTIRNFLDTEEFLEVETPILTKSTPEGARD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCCCCCHH
YLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
CCCCCEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
ASFLTQEEILDMMERMMTKVMKDVKGVEISVPFPRMTYADAMARYGSDKPDTRFEMELTD
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEEHHH
LSEFAAGCGFKVFTGAVENGGQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKV
HHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EEDGLKGPIAKFFNEEEANVIMTTLEASAGDLLLFVADKKSVVADSLGALRLRLGKELEL
CCCCCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEE
IDENKFNFLWVTDWPLLEYDEEANRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
EECCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHEEEEE
NGYELGGGSLRIYERDVQEKMFKALGFTQEEAREQFGFLLEAFEYGTPPHGGIALGLDRL
ECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGRTNLRDTIAFPKTASASCLLTDAPSPVAGAQLEELHLKLNVKE
HHHHCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
AERTHACGRVTVEAIGQTVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVFSPETSKEAL
CCCCCCCCCEEHHHCCCEEEEHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHH
EVAETIRGEYVLHVEGTVVARGAGAINENMATGQIEVQATKVTVLNAAKTTPIIIADDTD
HHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCC
ASEDVRLKYRYLDLRRPVMYNTFKMRHDVTKTIRNFLDTEEFLEVETPILTKSTPEGARD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEECCCCCCCHH
YLVPSRVHDGEFYALPQSPQLFKQLLMVGGFERYYQVARCFRDEDLRADRQPEFTQIDIE
CCCCCEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
ASFLTQEEILDMMERMMTKVMKDVKGVEISVPFPRMTYADAMARYGSDKPDTRFEMELTD
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEEHHH
LSEFAAGCGFKVFTGAVENGGQVKAINAKGAASKYSRKDIDALTEFVKVYGAKGLAWLKV
HHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EEDGLKGPIAKFFNEEEANVIMTTLEASAGDLLLFVADKKSVVADSLGALRLRLGKELEL
CCCCCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEE
IDENKFNFLWVTDWPLLEYDEEANRYFAAHHPFTMPFREDVELLETAPEKARAQAYDLVL
EECCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHEEEEE
NGYELGGGSLRIYERDVQEKMFKALGFTQEEAREQFGFLLEAFEYGTPPHGGIALGLDRL
ECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VMLLAGRTNLRDTIAFPKTASASCLLTDAPSPVAGAQLEELHLKLNVKE
HHHHCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA