Click here to switch to the map view.

The map label for this gene is hisS [H]

Identifier: 152976821

GI number: 152976821

Start: 3164431

End: 3165702

Strand: Reverse

Name: hisS [H]

Synonym: Bcer98_3119

Alternate gene names: 152976821

Gene position: 3165702-3164431 (Counterclockwise)

Preceding gene: 152976822

Following gene: 152976820

Centisome position: 77.46

GC content: 38.6

Gene sequence:

>1272_bases
ATGTCTATTCAAATCCCACGCGGAACGCAAGATATTCTTCCAGGTAGTGTAGAATTATGGCAATATATCGAAGGACAAGC
GCGCGAAATTTGCCGTCGTTACAATTATAAAGAAATTCGTACACCAATTTTTGAACATACAGAGCTGTTTTTACGCGGCG
TTGGTGATACAACAGATATTGTTCAAAAAGAAATGTACACATTCCAAGATCGCGGAGACCGCAGTTTAACACTTCGTCCA
GAAGGTACTGCACCTGTTGTGCGTTCTTATGTTGAAAATAAAATGTTCGGTGATGCAACACAGCCAACGAAATTATACTA
TATCGGTCAAATGTTCCGTTATGAAAGACCGCAAGCAGGTCGTTATCGTCAATTCGTCCAATTCGGTATTGAAGCAATCG
GTAGTAACGATCCTGCAATTGATGCCGAAGTAATTGCACTTGCGGTAGAATTTTACCGCGGTATGGGTTTAAAAAATATT
AAAGTTGTATTAAATAGCTTAGGAGATCCAGCGAGCCGTCAAGCGCATCGAGAAGCGTTAATCGCTCACTTTAAACCGCG
CATCGGTGAGTTTTGTTCCGATTGCCAATCTCGTTTAGAGAAAAACCCACTTCGTATTTTAGACTGTAAGAGAGATCGTG
ATCATGAATTAATGAATACAGCACCATCTATTACAGATTATTTAAACGAAGAATCAACGGCATATTATGAAAAGGTTCAA
GAGCTATTAACAATGATGGGCGTTCCATTTGAAAAAGATCCAAACTTAGTGCGTGGTTTAGATTATTATCAACACACTGT
TTTTGAAATTATGAGTGAAGCGGAAGGATTTGGTGCCATTACAACATTAAGTGGTGGTGGACGTTATAATGGACTTGTTC
AAGAAATCGGTGGCCCAGAAATGCCTGGTATCGGTTTTGCGATGAGTATTGAACGTTTAATTATGGCACTAAAAGCTGAA
AATATCGAGTTGCCAATTGAAAATACAATTGATTGTTACGTTGTTGCACTTGGTGATAAGGCGAAGGATCATGCTGCAAA
AGTTGCTTTTGACCTTCGTAAATCTGGCCTATCAGTTGAAAAAGATTATTTAAATCGTAAAATGAAAGCACAATTTAAAT
CAGCGGATCGTCTAAATGCGAAATATGTAGCGGTATTAGGTGAAGATGAGCTAGATAAAGGTGTGATTAATTTGAAGGAT
ATGGCGACAGGCGAGCAAGAAGAAGTAGCATTAGATGTATTTGCTTCATATATCGCAGAGAAATTAATATAG

Upstream 100 bases:

>100_bases
GCACCACGGGTATTATACTCTCGTCCCTACTGGATAATCAGTAGAGTCGAGAGTTTTTTTATTTTTATTAAGATTAAAAT
AATTTGAGGAGGAACTGAAT

Downstream 100 bases:

>100_bases
GGGGAACTTGTAATGGCTGAAAGAACACATGCATGTGGAAGAGTAACAGTAGAAGCAATTGGACAAACCGTTCAATTAAA
AGGTTGGGTACAAAAGCGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase 2; HisRS 2 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIQIPRGTQDILPGSVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYTFQDRGDRSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDPASRQAHREALIAHFKPRIGEFCSDCQSRLEKNPLRILDCKRDRDHELMNTAPSITDYLNEESTAYYEKVQ
ELLTMMGVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIENTIDCYVVALGDKAKDHAAKVAFDLRKSGLSVEKDYLNRKMKAQFKSADRLNAKYVAVLGEDELDKGVINLKD
MATGEQEEVALDVFASYIAEKLI

Sequences:

>Translated_423_residues
MSIQIPRGTQDILPGSVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYTFQDRGDRSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDPASRQAHREALIAHFKPRIGEFCSDCQSRLEKNPLRILDCKRDRDHELMNTAPSITDYLNEESTAYYEKVQ
ELLTMMGVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIENTIDCYVVALGDKAKDHAAKVAFDLRKSGLSVEKDYLNRKMKAQFKSADRLNAKYVAVLGEDELDKGVINLKD
MATGEQEEVALDVFASYIAEKLI
>Mature_422_residues
SIQIPRGTQDILPGSVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYTFQDRGDRSLTLRPE
GTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIK
VVLNSLGDPASRQAHREALIAHFKPRIGEFCSDCQSRLEKNPLRILDCKRDRDHELMNTAPSITDYLNEESTAYYEKVQE
LLTMMGVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAEN
IELPIENTIDCYVVALGDKAKDHAAKVAFDLRKSGLSVEKDYLNRKMKAQFKSADRLNAKYVAVLGEDELDKGVINLKDM
ATGEQEEVALDVFASYIAEKLI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=472, Percent_Identity=24.364406779661, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI6996014, Length=454, Percent_Identity=24.2290748898678, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=42.3809523809524, Blast_Score=347, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI71993693, Length=476, Percent_Identity=24.3697478991597, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=476, Percent_Identity=24.3697478991597, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=475, Percent_Identity=22.5263157894737, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643061, Length=480, Percent_Identity=24.375, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643059, Length=499, Percent_Identity=23.4468937875752, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47826; Mature: 47695

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIPRGTQDILPGSVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
CEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYTFQDRGDRSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDPASRQAHREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
IAHFKPRIGEFCSDCQSRLEKNPLRILDCKRDRDHELMNTAPSITDYLNEESTAYYEKVQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHH
ELLTMMGVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIENTIDCYVVALGDKAKDHAAKVAFDLRKSGLSVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
KDYLNRKMKAQFKSADRLNAKYVAVLGEDELDKGVINLKDMATGEQEEVALDVFASYIAE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC
>Mature Secondary Structure 
SIQIPRGTQDILPGSVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
EECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYTFQDRGDRSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDPASRQAHREAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
IAHFKPRIGEFCSDCQSRLEKNPLRILDCKRDRDHELMNTAPSITDYLNEESTAYYEKVQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHH
ELLTMMGVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIENTIDCYVVALGDKAKDHAAKVAFDLRKSGLSVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
KDYLNRKMKAQFKSADRLNAKYVAVLGEDELDKGVINLKDMATGEQEEVALDVFASYIAE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]