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The map label for this gene is ddl

Identifier: 152974076

GI number: 152974076

Start: 243252

End: 244340

Strand: Direct

Name: ddl

Synonym: Bcer98_0232

Alternate gene names: 152974076

Gene position: 243252-244340 (Clockwise)

Preceding gene: 152974075

Following gene: 152974077

Centisome position: 5.95

GC content: 36.27

Gene sequence:

>1089_bases
TTGACGAAAATTAAATTAGGTTTATTATATGGTGGGAAATCAGCAGAGCATCAAGTTTCATTACAAACAGCTCTAGCTGC
TATTAAAGCATTAAATCAAGAAAAATTCGAGATTCATCCAATTTATATTACAGAGCAAGGCCAATGGATGCGTGGGGAAC
GCATTGAAGGCGAAGTAACAAGTGTGCAAGCTTTACAAATGAGTGGAGAAGCGAATGCCATTTCTCCGGTATCACTAAGT
ACAGAAATTATCCCTGCACCTGCTTCTAAGCAAGAGGAAGCAATTGATGTTATTTTCCCATTGCTACACGGGCCAAACGG
TGAAGATGGAACGGTACAAGGACTATTAGAATTAATGAATATACCATATGTTGGGAATGGTGTGTTAGCATCAGCTGCTG
GTATGGATAAAGTTGTGATGAAAAATATTTTTGCAGAGGCTGGATTAAACCAGGCAAAATATACATCTTTCGTTCGCAGT
GTATGGGAGAAAAATCGTGAAGAAGCTTATGAAAAAGTAGAAGAAGCGCTAGGATATCCATGTTTTGTAAAACCAGCAAA
CCTTGGATCAAGCGTTGGTATTAATAAATGTAAAGATCGTGAAGAATTAGAGAAAGCATTTGAGGAAGCTTTCCAATTTG
ACCGTAAAATTATTGTAGAAGAAAATATTATCGGTCGTGAAGTGGAAGTGGGCGTTTTAGGAAATGATGATCCGAAATGT
TCTGTTGTAGGTGAAATCGTACCTAAGAAAGAATTCTATGATTATAAATCGAAATATATTGATGGTGATACAGCATTAAT
TATCCCTGCTGAAATTACAGAAGAAGAAACAGCTACAATTCAACGAGATGCAATTCGTGCATTCCAAGCTTTGGATGGTG
CGGGTTTAACAAGAGCTGACTTCTTTTTAACAAAAGATGGCGAAGTTTATATTAATGAAGTAAATACAATGCCAGGATTT
ACACCATTTAGCATGTTCCCGTTATTATGGCAACATACGGGGTTGTCATATCCAGAGTTAATTGAAGAATTAATTCGTTT
AGCAATTGAGCGTCATAAGGAAAAACAAAAAATTAAATATACAATCTAA

Upstream 100 bases:

>100_bases
TATTATTTGTGGAAGTTTCATTTTATTTCAAATAGAACTACCTATTTTTATAAAAAGTGCTAAAATAGAAAAATGAAAAC
AATTGGTTAGGGTGATTTCA

Downstream 100 bases:

>100_bases
CAAAAAAAGGGGGAAGCACTATTCACAAGAATAGTGCTTCCCCTATATAAGGAGTGTTTCATATGATAAAAAGAACGTTA
AAGCAAATCGAGAAAATGGT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVTSVQALQMSGEANAISPVSLS
TEIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRS
VWEKNREEAYEKVEEALGYPCFVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKC
SVVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRADFFLTKDGEVYINEVNTMPGF
TPFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKYTI

Sequences:

>Translated_362_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVTSVQALQMSGEANAISPVSLS
TEIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRS
VWEKNREEAYEKVEEALGYPCFVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKC
SVVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRADFFLTKDGEVYINEVNTMPGF
TPFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKYTI
>Mature_361_residues
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVTSVQALQMSGEANAISPVSLST
EIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRSV
WEKNREEAYEKVEEALGYPCFVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKCS
VVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRADFFLTKDGEVYINEVNTMPGFT
PFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKYTI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=368, Percent_Identity=44.8369565217391, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1786280, Length=345, Percent_Identity=32.1739130434783, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BACCN (A7GKE0)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373593.1
- ProteinModelPortal:   A7GKE0
- SMR:   A7GKE0
- STRING:   A7GKE0
- EnsemblBacteria:   EBBACT00000039175
- GeneID:   5344614
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0232
- eggNOG:   COG1181
- GeneTree:   EBGT00050000001406
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   BCER315749:BCER98_0232-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40270; Mature: 40139

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVT
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHH
SVQALQMSGEANAISPVSLSTEIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMN
HEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
IPYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRSVWEKNREEAYEKVEEALGYP
CCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
CFVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKC
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCE
SVVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRAD
EEHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHE
FFLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKY
EEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEC
TI
CC
>Mature Secondary Structure 
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVT
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCHH
SVQALQMSGEANAISPVSLSTEIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMN
HEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
IPYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRSVWEKNREEAYEKVEEALGYP
CCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
CFVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKC
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCE
SVVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRAD
EEHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHE
FFLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKY
EEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEC
TI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA