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The map label for this gene is murF [H]

Identifier: 152974077

GI number: 152974077

Start: 244403

End: 245779

Strand: Direct

Name: murF [H]

Synonym: Bcer98_0233

Alternate gene names: 152974077

Gene position: 244403-245779 (Clockwise)

Preceding gene: 152974076

Following gene: 152974078

Centisome position: 5.98

GC content: 36.6

Gene sequence:

>1377_bases
ATGATAAAAAGAACGTTAAAGCAAATCGAGAAAATGGTAAATGGAACAGGCTTAGCAGAGAAATATTTTGATATGACTGC
GCAAGGTGTTTCAATTGATACACGGAAAATGGAAAAAGGGAATGTATATGTGCCAATTCAAGGTGAACGTTTTGATGGTC
ATTCATTTGTAGAGAAAGCAATAGAGAGCGGTGCTGTAGCTACACTTTGGAAAAGAGATGTACAAAACCCACCTGCGAAT
ATGCCAGTTATCTTTGTGGAAGATACATTGGAAGCATTGCAAATGTTAGCTAAAAACTATCGTGATCAATTGAATGTAAA
AGTTGTTGGGGTAACAGGAAGTAATGGTAAGACATCTACAAAAGATATTGTGACGAGTCTTCTTGCAACTAAATTTAAAG
TCCAAAAAACAGAAGGTAATTTTAACAATCATATCGGCCTACCTCTTACGATTTTAAATTTGGAAGAGGATACAGAAATT
GCTGTTTTAGAAATGGGAATGTCTAGTAGAGGAGAAATTGAATTTTTATCTAAGTTAGCTCGTCCGAATGCAGCGATTAT
TACAAACATTGGCGAAGCTCATTTGATGGATTTAGGTTCTCGAGATGCAATTGCAGAAGCGAAGCTTGAGATTGTTACCG
GATTACAAGAAGGTGGAGTATTTGTATATAACGGGGATGAGCCTTTATTAACAAATCGCGTACCGAAGATGGATTTAGCG
GTAGAGACGATTACATTTGGAGATGCTAGAGCGAATGATTATTATCCAACTTCTGTGACATTACAAGCAACTGGGACGCA
TTTTACAATGAACCGAGATGAAAATGTATTGTTCTATTTGCCTGTTCTTGGGAAGCATAATGTATATAATGCACTTGCAT
CGATGGCAATTGCGAAATACTTTGGGGTGACATGGGAAGAAATGAAACAAGGTCTAGTGACGCTCCAAATGACAGGAATG
CGTATGGAAGTTGTAAAAACAAATAGCGGATTAACAATTATTAATGATGCGTACAATGCAAGTCCAACTGCAATGGAAGC
TGCTTTTCATTTGATGAATGGTTTAGATGGGTTTGCTAAAAAGATTGTTGTTCTGGGTGATATGTTAGAACTTGGAGATC
AAGAAGTACAATTTCATTATGAGGTAGGTAAGCTCATTGATCCTGCACGAATTTCTTATGTATTCACATATGGTAGATTA
GGAGCTCAAATTGCTGAAGGAGCAAAAATTAATTTCCCAAATGAACGTGTGAAGGCGTATGACAATAAGGAAGAATTAGT
GAAAAATCTAAAAGATATTGTAGATATGGAAGATGTCGTGTTGGTGAAAGCATCTCGCGGGATGAAAATGGAAGAAATCA
TTACAATGTTGAAATAA

Upstream 100 bases:

>100_bases
GTCATAAGGAAAAACAAAAAATTAAATATACAATCTAACAAAAAAAGGGGGAAGCACTATTCACAAGAATAGTGCTTCCC
CTATATAAGGAGTGTTTCAT

Downstream 100 bases:

>100_bases
TTGGGGTATATAAATAGGAATGTTCATCACGAAAATAGCAGAAACTTATAATAAGCGCTTACAGTGAAAGTTGAAAAGAG
AAAGTGAAGAAAAAAGCCCT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MIKRTLKQIEKMVNGTGLAEKYFDMTAQGVSIDTRKMEKGNVYVPIQGERFDGHSFVEKAIESGAVATLWKRDVQNPPAN
MPVIFVEDTLEALQMLAKNYRDQLNVKVVGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEEDTEI
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSRDAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPKMDLA
VETITFGDARANDYYPTSVTLQATGTHFTMNRDENVLFYLPVLGKHNVYNALASMAIAKYFGVTWEEMKQGLVTLQMTGM
RMEVVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFAKKIVVLGDMLELGDQEVQFHYEVGKLIDPARISYVFTYGRL
GAQIAEGAKINFPNERVKAYDNKEELVKNLKDIVDMEDVVLVKASRGMKMEEIITMLK

Sequences:

>Translated_458_residues
MIKRTLKQIEKMVNGTGLAEKYFDMTAQGVSIDTRKMEKGNVYVPIQGERFDGHSFVEKAIESGAVATLWKRDVQNPPAN
MPVIFVEDTLEALQMLAKNYRDQLNVKVVGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEEDTEI
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSRDAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPKMDLA
VETITFGDARANDYYPTSVTLQATGTHFTMNRDENVLFYLPVLGKHNVYNALASMAIAKYFGVTWEEMKQGLVTLQMTGM
RMEVVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFAKKIVVLGDMLELGDQEVQFHYEVGKLIDPARISYVFTYGRL
GAQIAEGAKINFPNERVKAYDNKEELVKNLKDIVDMEDVVLVKASRGMKMEEIITMLK
>Mature_458_residues
MIKRTLKQIEKMVNGTGLAEKYFDMTAQGVSIDTRKMEKGNVYVPIQGERFDGHSFVEKAIESGAVATLWKRDVQNPPAN
MPVIFVEDTLEALQMLAKNYRDQLNVKVVGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEEDTEI
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSRDAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPKMDLA
VETITFGDARANDYYPTSVTLQATGTHFTMNRDENVLFYLPVLGKHNVYNALASMAIAKYFGVTWEEMKQGLVTLQMTGM
RMEVVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFAKKIVVLGDMLELGDQEVQFHYEVGKLIDPARISYVFTYGRL
GAQIAEGAKINFPNERVKAYDNKEELVKNLKDIVDMEDVVLVKASRGMKMEEIITMLK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50744; Mature: 50744

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRTLKQIEKMVNGTGLAEKYFDMTAQGVSIDTRKMEKGNVYVPIQGERFDGHSFVEKA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHEECCCCCEEEEEECCCCCCHHHHHHH
IESGAVATLWKRDVQNPPANMPVIFVEDTLEALQMLAKNYRDQLNVKVVGVTGSNGKTST
HHCCCEEHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
KDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEEDTEIAVLEMGMSSRGEIEFLSKLA
HHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAIITNIGEAHLMDLGSRDAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPKMDLA
CCCEEEEECCCCHHHHCCCCCCHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCCCCEE
VETITFGDARANDYYPTSVTLQATGTHFTMNRDENVLFYLPVLGKHNVYNALASMAIAKY
EEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHH
FGVTWEEMKQGLVTLQMTGMRMEVVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFAK
HCCCHHHHHCCEEEEEECCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
KIVVLGDMLELGDQEVQFHYEVGKLIDPARISYVFTYGRLGAQIAEGAKINFPNERVKAY
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEHHHCHHHHCCCEECCCHHHHHCC
DNKEELVKNLKDIVDMEDVVLVKASRGMKMEEIITMLK
CCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MIKRTLKQIEKMVNGTGLAEKYFDMTAQGVSIDTRKMEKGNVYVPIQGERFDGHSFVEKA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEHEECCCCCEEEEEECCCCCCHHHHHHH
IESGAVATLWKRDVQNPPANMPVIFVEDTLEALQMLAKNYRDQLNVKVVGVTGSNGKTST
HHCCCEEHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
KDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEEDTEIAVLEMGMSSRGEIEFLSKLA
HHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAIITNIGEAHLMDLGSRDAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPKMDLA
CCCEEEEECCCCHHHHCCCCCCHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCCCCEE
VETITFGDARANDYYPTSVTLQATGTHFTMNRDENVLFYLPVLGKHNVYNALASMAIAKY
EEEEEECCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHH
FGVTWEEMKQGLVTLQMTGMRMEVVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFAK
HCCCHHHHHCCEEEEEECCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
KIVVLGDMLELGDQEVQFHYEVGKLIDPARISYVFTYGRLGAQIAEGAKINFPNERVKAY
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEEHHHCHHHHCCCEECCCHHHHHCC
DNKEELVKNLKDIVDMEDVVLVKASRGMKMEEIITMLK
CCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]