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The map label for this gene is gltX [H]

Identifier: 152973935

GI number: 152973935

Start: 103575

End: 105032

Strand: Direct

Name: gltX [H]

Synonym: Bcer98_0082

Alternate gene names: 152973935

Gene position: 103575-105032 (Clockwise)

Preceding gene: 152973934

Following gene: 152973936

Centisome position: 2.53

GC content: 37.52

Gene sequence:

>1458_bases
ATGGAAAAGCAAGTGAGAGTGCGCTATGCGCCAAGTCCAACAGGACACTTACATATCGGAAATGCGCGTACGGCATTATT
TAATTATTTATTTGCTCGTCATCAAGATGGTAAGTTTATTATTCGCATTGAAGATACTGATGTGAAACGTAACGTTGCTG
GCGGTGAAGAAAGCCAATTAAAATACTTGAAATGGCTCGGTATGGACTGGGATGAAGGTGTTGACGTTGGTGGTAAATAT
GGACCATATCGTCAAACAGAGCGTTTAGATATTTATAAAGAGTTATATGAAGATTTACTAGAGCGTGGTTTAGCTTATAA
ATGCTATATGACTGAGGAAGAATTAGAAGCAGAACGTGAAGGGCAAATCGCTCGTGGTGAGACACCTCGTTACGCAGGTA
ATCACCGCAATTTAACAGAGGAACAAATTGCGCAATTTGAGGCGGAAGGCCGTGTTCCAAGTATTCGCTTCCGTGTACCA
GATAACCGTGAGTACAAATTTAATGATATTGTAAAAGGTGATGTGACATTCCATTCTAACGACTTCGGTGACTTTGTTAT
CGTGAAAAAAGATGGTATTCCAACATATAACTTTGCGGTAGCGGTAGATGATCATTTAATGGAAATTACACATGTTCTTC
GTGGAGATGATCATATTTCGAATACGCCAAAACAAATGATGATTTATGAAGCATTTGGCTGGGATACTCCTCAGTTTGGT
CATATGACACTCATCGTAAATGAAAGTCGTAAAAAATTAAGTAAACGAGATGAATCTATTATTCAATTTATTGAACAATA
TGAAGCACTTGGATATCTTCCAGAAGCAATTTTTAACTTCATTGCATTACTAGGTTGGTCACCAGTAGGAGAAGAGGAAA
TCTTCTCAAAAGATGAGTTTATTAAGATGTTTGATGCAGCTCGTTTATCAAAATCACCTGCGTTATTCGATTCCCAAAAA
TTAAAATGGATGAATAACCAATATATGAAAAAACAAGATTTAGATACTGTTGTTGCATTAAGCTTACCGCATTTAGTGAA
AGCTGGTCGTGTAAGTGAAGATTTAAGTGAGCAAGAAGCAGCTTGGGTTCGGGACGTAATTGCTTTATATCATGAACAAA
TGAGCTATGGGGCTGAAATTGTGGAGCTTTCTGAAATGTTCTTCAAAGATCATGTAGACTTTGAAGAAGAAGGAAAAGAA
GTATTAAAAGGAGAACAAGTACCTGAAGTGCTTCGTGCGTTTGCAGTAGAATTAGAGGCTCTAGAGGAAGTAGAACCAGC
TACAATTAAGAAAGCAATTAAAGCTGTTCAAAAGGAAACAGGACATAAGGGTAAAAACTTATTTATGCCAATTCGTGTTG
CAACAACTGGGCAAACACATGGTCCAGAGCTTCCAAATGCGATTGCGCTTCTTGGAAAAGAGAAAGTCTTAAAACGTATT
CAAAAAGTAATTGGTTAA

Upstream 100 bases:

>100_bases
AGAAAAAATAATGGTTTTTTGTTTGCAAGATGGATAATCAGATTTTTTTGGTGTACAATTATAGAATGTGTTGACATTAA
GAATGGAAGGTGTACCAATT

Downstream 100 bases:

>100_bases
CATTTAACGAATTTCGAAATATAATAAGAATACTCAAAAGTGAATAAGGAAAAGCGACGAGAAGGAGAAGTAGAAAAACA
GGCTTTTTACAGAGAGAACC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGVDVGGKY
GPYRQTERLDIYKELYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRNLTEEQIAQFEAEGRVPSIRFRVP
DNREYKFNDIVKGDVTFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGWDTPQFG
HMTLIVNESRKKLSKRDESIIQFIEQYEALGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPALFDSQK
LKWMNNQYMKKQDLDTVVALSLPHLVKAGRVSEDLSEQEAAWVRDVIALYHEQMSYGAEIVELSEMFFKDHVDFEEEGKE
VLKGEQVPEVLRAFAVELEALEEVEPATIKKAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKEKVLKRI
QKVIG

Sequences:

>Translated_485_residues
MEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGVDVGGKY
GPYRQTERLDIYKELYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRNLTEEQIAQFEAEGRVPSIRFRVP
DNREYKFNDIVKGDVTFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGWDTPQFG
HMTLIVNESRKKLSKRDESIIQFIEQYEALGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPALFDSQK
LKWMNNQYMKKQDLDTVVALSLPHLVKAGRVSEDLSEQEAAWVRDVIALYHEQMSYGAEIVELSEMFFKDHVDFEEEGKE
VLKGEQVPEVLRAFAVELEALEEVEPATIKKAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKEKVLKRI
QKVIG
>Mature_485_residues
MEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMDWDEGVDVGGKY
GPYRQTERLDIYKELYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRNLTEEQIAQFEAEGRVPSIRFRVP
DNREYKFNDIVKGDVTFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGWDTPQFG
HMTLIVNESRKKLSKRDESIIQFIEQYEALGYLPEAIFNFIALLGWSPVGEEEIFSKDEFIKMFDAARLSKSPALFDSQK
LKWMNNQYMKKQDLDTVVALSLPHLVKAGRVSEDLSEQEAAWVRDVIALYHEQMSYGAEIVELSEMFFKDHVDFEEEGKE
VLKGEQVPEVLRAFAVELEALEEVEPATIKKAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKEKVLKRI
QKVIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=32.9959514170041, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4826960, Length=320, Percent_Identity=25, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=484, Percent_Identity=36.9834710743802, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=34.5098039215686, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=34.020618556701, Blast_Score=252, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=33.6653386454183, Blast_Score=249, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=323, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=27.6497695852535, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=33.7373737373737, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI17864488, Length=306, Percent_Identity=25.4901960784314, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55662; Mature: 55662

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCHHHH
KYLKWLGMDWDEGVDVGGKYGPYRQTERLDIYKELYEDLLERGLAYKCYMTEEELEAERE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHC
GQIARGETPRYAGNHRNLTEEQIAQFEAEGRVPSIRFRVPDNREYKFNDIVKGDVTFHSN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCEEECCEEEECC
DFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGWDTPQFG
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCCC
HMTLIVNESRKKLSKRDESIIQFIEQYEALGYLPEAIFNFIALLGWSPVGEEEIFSKDEF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCHHHH
IKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVALSLPHLVKAGRVSEDLSEQEA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AWVRDVIALYHEQMSYGAEIVELSEMFFKDHVDFEEEGKEVLKGEQVPEVLRAFAVELEA
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEEVEPATIKKAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKEKVLKRI
HHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCHHHHHHHH
QKVIG
HHHHC
>Mature Secondary Structure
MEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCHHHH
KYLKWLGMDWDEGVDVGGKYGPYRQTERLDIYKELYEDLLERGLAYKCYMTEEELEAERE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHC
GQIARGETPRYAGNHRNLTEEQIAQFEAEGRVPSIRFRVPDNREYKFNDIVKGDVTFHSN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEECCEEECCEEEECC
DFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIYEAFGWDTPQFG
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCCC
HMTLIVNESRKKLSKRDESIIQFIEQYEALGYLPEAIFNFIALLGWSPVGEEEIFSKDEF
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCHHHH
IKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVALSLPHLVKAGRVSEDLSEQEA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AWVRDVIALYHEQMSYGAEIVELSEMFFKDHVDFEEEGKEVLKGEQVPEVLRAFAVELEA
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEEVEPATIKKAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIALLGKEKVLKRI
HHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCHHHHHHHH
QKVIG
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA