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The map label for this gene is ispF [H]

Identifier: 152973934

GI number: 152973934

Start: 103009

End: 103485

Strand: Direct

Name: ispF [H]

Synonym: Bcer98_0081

Alternate gene names: 152973934

Gene position: 103009-103485 (Clockwise)

Preceding gene: 152973933

Following gene: 152973935

Centisome position: 2.52

GC content: 37.74

Gene sequence:

>477_bases
ATGTTTCGAATTGGACAAGGTTTTGATGTGCATGAATTTGCAGAAGGAAGACCGTTAATTATCGGAGGGATTACGATTCC
ATATGAGAAAGGTTTATTAGGTCATTCAGATGCAGATGTGCTTTTACATACAATTGCGGATGCTTGTTTAGGAGCGATTG
CTGCTGGAGATATCGGAAAACATTTTCCAGACACAGACCCGGCTTTTAAAGATGCGGATTCGGCAGTGTTATTACAAAAG
GTTTGGGAGTTTGTACGTGAACAAGGATATGAATTAGGGAATTTAGATTGTACAATTATTGCTCAGAAACCAAAGATGGC
GCCACATATTGAAAGCATGCGGAAACGTATTAGTGAGCTTTTAGAAACATCGATTGATAATATTAATGTAAAAGCGACAA
CGACAGAAAAGTTGGGATTTACAGGTAGAGAAGAAGGAATTGCTTCTCAAGCGGTTGTATTATTACAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGTTGAAAAGCTCGGTTCGTACCGGGCTTTTCTTTATACACATAGCGATATAAGATAGAATCATAAAATTTAGTAGTTTA
GCTTGAGGAGGATGTATAGA

Downstream 100 bases:

>100_bases
TGGTTTTTTGTTTGCAAGATGGATAATCAGATTTTTTTGGTGTACAATTATAGAATGTGTTGACATTAAGAATGGAAGGT
GTACCAATTATGGAAAAGCA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Sequences:

>Translated_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
>Mature_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=65.3846153846154, Blast_Score=191, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17245; Mature: 17245

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPYEKGLLGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWEFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA