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The map label for this gene is ispD

Identifier: 152973933

GI number: 152973933

Start: 102219

End: 102899

Strand: Direct

Name: ispD

Synonym: Bcer98_0080

Alternate gene names: 152973933

Gene position: 102219-102899 (Clockwise)

Preceding gene: 152973932

Following gene: 152973934

Centisome position: 2.5

GC content: 38.62

Gene sequence:

>681_bases
ATGTATACATTAATTATTCCAGCAGCAGGTCAAGGGAAACGAATGGGCGCTGGTAAAAACAAATTGTTTTTGCTTATTAA
TGAGGTGCCTATTATCGTTCATACATTACGTGCTTTTGAGAAAGACGAAGCATGTGAAAAAATTATTATGGCAATTAACG
AACAAGAGCGTTCTGATTTTGAGGCATTAATACAAAAGTACGGTATTCGAAAGAATGTGCAGTTTATTCAAGGTGGAGCC
GAAAGACAAGATAGTGTGTATCATGCACTTCAGTATGTGAAAGAAACTGAATATGTTCTTGTTCATGATGGTGCCCGCCC
TTTTGTAACGAATAAGATGATTCATGAAGTATTAATTGTGGCAAAAGAGAAGGGTGCTTCCATTTGCGCTGTACCAGTGA
AGGATACGATAAAGAAAGTAGTAAATGATGCAGTTTTTGAGACAGTTGAACGTTCTCAACTTAGAGCGGTTCAAACACCG
CAAGGCTTTTCTGTTGCACTTTTATTAGAGGCTCATCAAAGCGCGAAGCAAAGTGGTTTTCTTGGTACGGATGATGCAAG
TCTCGTAGAGCGTATCGGAAAGGAAGTCGGCGTAGTAGAAGGTAGCTATTATAATATTAAGGTGACAACACCAGAAGATT
TATTAATTGCTGAAAGCTTTTTGCACGTTCAGAGAAGATAG

Upstream 100 bases:

>100_bases
GAAGTACTTGTTACAAGTGTACTGCAAACATCGGCTGGTCGTATGATTTTTGCAAAGCGTAAATTATTAGAAAAAGCATT
ATAAGCAGAGGATTCGTAAT

Downstream 100 bases:

>100_bases
CGGTAGTATGGTTGAAAAGCTCGGTTCGTACCGGGCTTTTCTTTATACACATAGCGATATAAGATAGAATCATAAAATTT
AGTAGTTTAGCTTGAGGAGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDEACEKIIMAINEQERSDFEALIQKYGIRKNVQFIQGGA
ERQDSVYHALQYVKETEYVLVHDGARPFVTNKMIHEVLIVAKEKGASICAVPVKDTIKKVVNDAVFETVERSQLRAVQTP
QGFSVALLLEAHQSAKQSGFLGTDDASLVERIGKEVGVVEGSYYNIKVTTPEDLLIAESFLHVQRR

Sequences:

>Translated_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDEACEKIIMAINEQERSDFEALIQKYGIRKNVQFIQGGA
ERQDSVYHALQYVKETEYVLVHDGARPFVTNKMIHEVLIVAKEKGASICAVPVKDTIKKVVNDAVFETVERSQLRAVQTP
QGFSVALLLEAHQSAKQSGFLGTDDASLVERIGKEVGVVEGSYYNIKVTTPEDLLIAESFLHVQRR
>Mature_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDEACEKIIMAINEQERSDFEALIQKYGIRKNVQFIQGGA
ERQDSVYHALQYVKETEYVLVHDGARPFVTNKMIHEVLIVAKEKGASICAVPVKDTIKKVVNDAVFETVERSQLRAVQTP
QGFSVALLLEAHQSAKQSGFLGTDDASLVERIGKEVGVVEGSYYNIKVTTPEDLLIAESFLHVQRR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=229, Percent_Identity=31.0043668122271, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI157671913, Length=132, Percent_Identity=32.5757575757576, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1789104, Length=220, Percent_Identity=34.5454545454545, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_BACCN (A7GJZ7)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373450.1
- ProteinModelPortal:   A7GJZ7
- SMR:   A7GJZ7
- STRING:   A7GJZ7
- EnsemblBacteria:   EBBACT00000039462
- GeneID:   5344347
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0080
- eggNOG:   COG1211
- GeneTree:   EBGT00050000001852
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   BCER315749:BCER98_0080-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25213; Mature: 25213

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDEACEKIIMAINEQERSDF
CEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EALIQKYGIRKNVQFIQGGAERQDSVYHALQYVKETEYVLVHDGARPFVTNKMIHEVLIV
HHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
AKEKGASICAVPVKDTIKKVVNDAVFETVERSQLRAVQTPQGFSVALLLEAHQSAKQSGF
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCC
LGTDDASLVERIGKEVGVVEGSYYNIKVTTPEDLLIAESFLHVQRR
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLINEVPIIVHTLRAFEKDEACEKIIMAINEQERSDF
CEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EALIQKYGIRKNVQFIQGGAERQDSVYHALQYVKETEYVLVHDGARPFVTNKMIHEVLIV
HHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
AKEKGASICAVPVKDTIKKVVNDAVFETVERSQLRAVQTPQGFSVALLLEAHQSAKQSGF
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCC
LGTDDASLVERIGKEVGVVEGSYYNIKVTTPEDLLIAESFLHVQRR
CCCCCHHHHHHHHHHHCEECCCEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA