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The map label for this gene is puuP [H]

Identifier: 152969578

GI number: 152969578

Start: 1172445

End: 1173836

Strand: Direct

Name: puuP [H]

Synonym: KPN_01022

Alternate gene names: 152969578

Gene position: 1172445-1173836 (Clockwise)

Preceding gene: 152969577

Following gene: 152969580

Centisome position: 22.06

GC content: 57.97

Gene sequence:

>1392_bases
ATGGCTATTAATCCTCCGGCGCATAACGCCGCTCAGGCGGGCAGACCGCGTCTGCGTAAATCCCTCAAACTCTGGCAAGT
GGTGATGATGGGGCTGGCCTATCTGACGCCAATGACGGTGTTTGATACCTTCGGCATCGTCTCCGGCATCAGCAATGGCC
ACGTTCCGGCGTCTTATCTGCTGGCGCTGGCTGGCGTGCTGTTCACCGCCATCAGCTACGGCAAACTGGTGCGTCAGTTT
CCGGAAGCCGGTTCCGCCTATACCTACGCGCAAAAATCCATCAGTCCGCACGTCGGCTTTATGGTTGGCTGGTCGTCGCT
GCTGGATTATCTGTTTCTGCCGATGATCAACGTCCTGTTGGCGAAGATCTATCTCTCGGCCTTGTTCCCCGAAGTGCCGC
CGTGGGTGTGGGTAGTGACCTTTGTCGCTATTCTGACCGCCGCGAACCTGAAAAGCGTTAACCTGGTGGCCAACTTCAAT
ACCCTGTTCGTGCTGGTGCAGATTTCGATCATGGTGGTGTTTGTCATTCTGGTGGTGCAGGGGCTGCATAAAGGCGAGGG
GGTGGGTACCGTCTGGTCGCTGCAGCCGTTTATCAGCCAGAATGCGCATTTGATCCCGATTATTACCGGCGCGACCATTG
TCTGCTTCTCGTTTCTCGGTTTCGACGCGGTGACCACCCTGTCGGAAGAGACCCCTGACGCCGCGCGGGTGATCCCGAAG
GCAATTTTTCTCACCGCGATGTACGGCGGCATTATCTTTATCGTCGCGTCGTTCTTTATGCAGCTGTTCTTCCCGGATAT
TCATCGCTTTAAAGACCCCGATGCCGCGCTGCCGGAGATTGCGCTGTACGTGGGCGGCAAGCTGTTCCAGTCGATTTTCC
TGTGCACCACCTTTGTTAACACCCTGGCGTCCGGCCTGGCCTCCCATGCCAGCGTCTCCCGTCTGCTGTACGTGATGGGC
CGCGACAACGTCTTCCCGGAGCGCATTTTTGGCTATGTGCATCCGAAATGGCGTACCCCGGCGCTGAACGTGATTATGGT
GGGCATCGTTGCGCTGTCGGCGCTGTTCTTCGACCTGGTGACCGCCACCGCGTTGATTAATTTCGGCGCGCTGGTGGCCT
TCACCTTCGTTAACCTGTCGGTGTTCAACCACTTCTGGCGGCGCAAGGGGTACAACAAAACCTGGAAGGATCGACTGCAT
TATCTGCTGCTGCCGATGGTCGGGGCGTTGACGGTCGGGGTATTGTGGATCAATCTCGAGGCCACCTCCCTGACGCTCGG
CCTGGTGTGGGCGGCGCTTGGTCTGCTGTACCTGACTTATCTGACCCGACGCTTCCGCAAACCGCCGCCGCAGTTTGATG
CCGCTAAAGCGGAGCAGGCCTGGGAGTCATAG

Upstream 100 bases:

>100_bases
TCCGTCTTACCCTTTTATCTATGCCTTACCCCTTTGCTAAGCAGGTTTGTCACGCGGACGTTGGGCCCGCAAACTGTTAA
CAGCAAGGGGGGGATGGGCG

Downstream 100 bases:

>100_bases
GTTCTGAGGCATAAACTGAACGAAGGCAGGCCCGCAGGCCTGCTTTCGTTTTGGCTTCGGTAAGGGACAGTACAGGACGG
TAAAGAAATCAGCAGAAAAC

Product: putative amino acid/amine transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAINPPAHNAAQAGRPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFVILVVQGLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAMYGGIIFIVASFFMQLFFPDIHRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGYNKTWKDRLH
YLLLPMVGALTVGVLWINLEATSLTLGLVWAALGLLYLTYLTRRFRKPPPQFDAAKAEQAWES

Sequences:

>Translated_463_residues
MAINPPAHNAAQAGRPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFVILVVQGLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAMYGGIIFIVASFFMQLFFPDIHRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGYNKTWKDRLH
YLLLPMVGALTVGVLWINLEATSLTLGLVWAALGLLYLTYLTRRFRKPPPQFDAAKAEQAWES
>Mature_462_residues
AINPPAHNAAQAGRPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYLLALAGVLFTAISYGKLVRQFP
EAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNT
LFVLVQISIMVVFVILVVQGLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPKA
IFLTAMYGGIIFIVASFFMQLFFPDIHRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGR
DNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGYNKTWKDRLHY
LLLPMVGALTVGVLWINLEATSLTLGLVWAALGLLYLTYLTRRFRKPPPQFDAAKAEQAWES

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI114326544, Length=423, Percent_Identity=22.2222222222222, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI114326550, Length=423, Percent_Identity=22.2222222222222, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4507047, Length=408, Percent_Identity=21.8137254901961, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081869, Length=449, Percent_Identity=92.8730512249443, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=448, Percent_Identity=64.5089285714286, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1788480, Length=399, Percent_Identity=25.062656641604, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786602, Length=198, Percent_Identity=24.7474747474747, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786789, Length=174, Percent_Identity=28.1609195402299, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081708, Length=455, Percent_Identity=24.6153846153846, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082250, Length=409, Percent_Identity=21.2713936430318, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786302, Length=173, Percent_Identity=26.0115606936416, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=406, Percent_Identity=23.6453201970443, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=404, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=401, Percent_Identity=25.1870324189526, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24667468, Length=397, Percent_Identity=25.1889168765743, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24668806, Length=411, Percent_Identity=25.5474452554745, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356285, Length=411, Percent_Identity=25.5474452554745, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24668802, Length=411, Percent_Identity=25.5474452554745, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281366235, Length=405, Percent_Identity=23.9506172839506, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI116007820, Length=405, Percent_Identity=23.9506172839506, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI116007818, Length=406, Percent_Identity=24.384236453202, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85725152, Length=406, Percent_Identity=24.384236453202, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221512776, Length=399, Percent_Identity=21.5538847117794, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24666159, Length=399, Percent_Identity=21.5538847117794, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51126; Mature: 50995

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINPPAHNAAQAGRPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYL
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFVILVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HCCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAMYGGIIFIVASFFMQLFFPDIHRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGYNKTWKDRLHYLLLPMVGALTVGVLWINLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
ATSLTLGLVWAALGLLYLTYLTRRFRKPPPQFDAAKAEQAWES
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
AINPPAHNAAQAGRPRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISNGHVPASYL
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSISPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFVILVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISQNAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HCCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAMYGGIIFIVASFFMQLFFPDIHRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERIFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGYNKTWKDRLHYLLLPMVGALTVGVLWINLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
ATSLTLGLVWAALGLLYLTYLTRRFRKPPPQFDAAKAEQAWES
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]