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The map label for this gene is purR [H]

Identifier: 152969411

GI number: 152969411

Start: 953849

End: 954922

Strand: Direct

Name: purR [H]

Synonym: KPN_00854

Alternate gene names: 152969411

Gene position: 953849-954922 (Clockwise)

Preceding gene: 152969408

Following gene: 152969418

Centisome position: 17.95

GC content: 64.34

Gene sequence:

>1074_bases
ATGGATAAAAAGCTGCGCGTCGCCGAAATTGCCGCCCGCACCGGGCTATCGCCCAGCACCGTTTCACGCGTCCTGGCCGG
GAAAGCGAATACCAGCGACAAGGCGCGCCGGGCGGTGCTGGAGTGCGCCCGCGAGCTGGGTGTCATGCAGGGGTTGGCCG
CCGGGCGTCTGCTGCTCAATCATCTGCTGGTGTTTGCCCCGCAGCGCGCATTCGATGAGCGATCGGATATCTTCTACTAC
CGGGTGATCCAGAGCATCAATAACGCGCTGGCGGCGCATGAAGTGCGCCTGCGCTACTGCGCGCTGGATGAGAACGACAG
CGACGCCAACCTGTTTCTTGCCCGCATGAATGAACCTGAGACCCAGGCCGCGGTGCTGCTGGGGATCGACGATCCGCATA
TTCATGGCCTGGCGGCGGATCTGGGCAAACCCTGCGTATTGATCAACTGTCGGGATGAAGGGATGCGGCTGCCGTCCATC
GCTCCCGATCATCGGCTGATCGGCCAGTGCGCGGCCCGCTATCTGTTTGATATGGGGCATCGGGCGGTGATGAACGTGAT
GTGCCTGCGCCGCTATACCATGGAGCTGCGGCTGGGGGGGATTCAGGAGGCGTGGCGCGCGCGTAATCTGCGCTTTACCG
CCAGCCGGGATCTTATCAGCGTCGCCAACTTTAGCGCCAGAGAGACGGAAGAGCAGGTGGGCGCCTGGCTTGACGGCTTA
CAGGGCAGGCCGTTGCCCACCGCCTTCCTGGTGGGCGGCGATTTTATGGCCGCCGGCACCGTCAGCGCATTGCAAAAGCG
TGGCCTGCGGGTGCCGCAGGATGTCTCGGTGATGAGCATTGACGGATTTAATCTGGCGGCGATTCAGGAGGTGCCGCTGA
CCGCGGTGCATGTGCCGCGGGATGAGCTGGGCAGCGAGGCGGTGCATCTCCTGCAGCAGCGCCTGCTGCGCCCGGAGGCG
CCGCACGGCTCGCTACTGCTGCATGGCACGCTGGTGGTACGCGATTCGGTACGGCGGATCCGGCCGGGCAAAGGCCATAC
CGCCGTCGAGCCGCAGGGGCTGTATGACGATTAG

Upstream 100 bases:

>100_bases
AATGAGCGAGATCGCATATTTGACAGCGGAATTTGCTTGTAACGTGTGCTTTGGTGATTATGAAAATTCTTTCATTTTTC
AATCGGAGCTGGATCACAAA

Downstream 100 bases:

>100_bases
ACCGGCATACCCAGCGCCCGTTTGCCGTGGATATTCAGATCCTCCAGGGTGAAGCGCCCCTGCCAGCTGGCTTCATAGTC
CTCGCGCGGGAAATCCCCCG

Product: putative periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MDKKLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLECARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYY
RVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSI
APDHRLIGQCAARYLFDMGHRAVMNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGL
QGRPLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEA
PHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD

Sequences:

>Translated_357_residues
MDKKLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLECARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYY
RVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSI
APDHRLIGQCAARYLFDMGHRAVMNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGL
QGRPLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEA
PHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD
>Mature_357_residues
MDKKLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLECARELGVMQGLAAGRLLLNHLLVFAPQRAFDERSDIFYY
RVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPETQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSI
APDHRLIGQCAARYLFDMGHRAVMNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGL
QGRPLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPRDELGSEAVHLLQQRLLRPEA
PHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=25.6716417910448, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39302; Mature: 39302

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLECARELGVMQGLAAGRLLLN
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLLVFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPE
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
TQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGH
CCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
RAVMNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHCC
QGRPLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPR
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCH
DELGSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDKKLRVAEIAARTGLSPSTVSRVLAGKANTSDKARRAVLECARELGVMQGLAAGRLLLN
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLLVFAPQRAFDERSDIFYYRVIQSINNALAAHEVRLRYCALDENDSDANLFLARMNEPE
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
TQAAVLLGIDDPHIHGLAADLGKPCVLINCRDEGMRLPSIAPDHRLIGQCAARYLFDMGH
CCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
RAVMNVMCLRRYTMELRLGGIQEAWRARNLRFTASRDLISVANFSARETEEQVGAWLDGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHCC
QGRPLPTAFLVGGDFMAAGTVSALQKRGLRVPQDVSVMSIDGFNLAAIQEVPLTAVHVPR
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCH
DELGSEAVHLLQQRLLRPEAPHGSLLLHGTLVVRDSVRRIRPGKGHTAVEPQGLYDD
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA