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The map label for this gene is pheT [H]

Identifier: 150398673

GI number: 150398673

Start: 3693533

End: 3695959

Strand: Direct

Name: pheT [H]

Synonym: Smed_3487

Alternate gene names: 150398673

Gene position: 3693533-3695959 (Clockwise)

Preceding gene: 150398672

Following gene: 150398676

Centisome position: 97.66

GC content: 64.48

Gene sequence:

>2427_bases
ATGAAGTTCACGCTCTCCTGGTTGAAGGACCATCTGGAAACCGAAGCCTCGCTCGAGGAAATCTGCGCCCGCCTGACGAT
GATCGGGCTGGAAGTCGAAGATGTCGACGACAAGGCGGCCTTCAAGCCGTTCGTCATTGCAAAAGTGATCTCCGCCGAGC
AGCACCCGAATGCCGACAAGCTCAGAGTGCTGATGGTCGATACCGGTTCCGGCGAGCCGGTGCAGGTGGTCTGCGGCGCA
CCGAACGCGCGCAAAGGCCTCGTCGGCGCCTTTGCCGCGCCCGGTACCTATGTGCCCGGCATCGGTGTGACACTCTCCGT
CGGCAACATTCGCGGCGTCGAAAGCCGCGGCATGATGTGCTCGGAAAAGGAGCTGGAGATCTCCGACGACCATAACGGCA
TCATCGATCTGCCCGAGGATGCGCCTGTAGGCGCCAGCTATGCGGATTTTGCCGGCCTCGACGATCCCGTCATCGCGATC
AACCTGACACCGAACCGGCCGGATTGCACCAGCGTCCACGGCATCGCCCGCGATCTCGCGGCATCCGGTCTCGGCACCTT
GAAGACGCGGCCGATGCCGTCCTTCGCCGTGGAAGGCGAGACGCCGGTCAAGGTCCGCCTCGATCTCGGCGAAGACGGGC
ATCTGTGCCCCGGCTTCGCGCTCCGCCTTGTGCGCGGCGTCAAGAACGGTCCGAGTCCCGCATGGATGCGCCGGCGGCTG
ATGGCCATCGGCTTGAGGCCGATCAATGCGCTGGTCGACATCACCAATTACCTGACCTTCGATCAGGGCCGGCCGCTTCA
CGTCTTCGACGCGGCAAAGGTTGCCGGCAACCTGACGGTTCGCCGTGCCAGGGAAGGTGAAAAGGTGTTGGCGCTCGATA
CGCGCGAATACATGCTGTCGCCCGCCAATGTGGTGATTGCCGATGAGGAAGGCGTTGAATCGATCGGCGGCATCATGGGC
GGTGAGCATTCCGGCTGCGACGAGACGACGACTGACGTGCTGATCGAATCGGCGCTTTGGGATCCGATGAACATCGCCAG
GACCGGCCGCACGCTCAGCATTATCACCGATGCGCGCTACCGCTTCGAACGCGGGGTCGATCCGGAATACATGGTGCCGG
GCCTCGAACGCGCCACCGAACTGGTACTGGAACTCTGCGGCGGGACCGCGGCGAGGATGGACGTCGTCGGTTATGACGGC
CACACGCCCAAGCGTGCAGATTTCCCCGTCAGCGAGGTGAAGCGCCTCACCGGCCTTGAGGTTTCCTCCGAGGAGAGCGT
CTCCATTCTCCGGAAGCTCGGCTTCGGCGTCGAGGGCTCCGGCGAACGCCTGACGGTGAAGGTGCCTTCCTGGCGGCCGG
ACATCGATGGCAAGGCGGACCTCGTCGAGGAAGTGATGCGCATCCATGGAGTCGACAATATCGTTGCCGCCCCGCTTGCG
AGCCATAATGCGGTCAACGGCAGGATCCTGACGGTGCTGCAGATCCGCACACGCCAGGCGAGGCGTGCTCTCGCGAGCCG
CGGCATGCTCGAGGCGGTTACATGGTCCTTCATCCCCCAAGAGCATGCGAAGCTCTTCGGGGGCGGCCAGCCGGAACTCA
AGCTTGCCAATCCGATCGCCGCCGACATGTCGGACATGCGGCCGTCGTTGCTGCCGGGTCTTCTGGCCGCGGCGCAGCGC
AACGCCGACAAAGGCCATGGGGATGTTGCGATCTTCGAGGTATCCGGTACCTATGAGGGCGACACGCCCGGGGCGCAGCG
CCGCGTCGCCGGCGGCGTGCGTCGCGGCACAGCCTCGCTCAACGGCGCCGGCCGGCTCTGGTCCAATTCGATGAAGGGCG
GCGGCAAGCCGGTCGATGTCTTCGATGCGAAGGCCGATGCGATCGCCGTGCTCGAAGCCTGCGGCGTGCCGATGGCTAAT
GTCCAGTTCGAAGCCGGTGGCCCGGCCTGGTACCATCCCGGCCGCTCGGCGACGATCAAGCTCGGACCGAAAATCGTCCT
CGGCGCTTTCGGAGAATTCCATCCGAAGACCCTCGACGTCCTTGATGTCTCCGGCCCGCTTTCAGGCTTCGAAGTCTATA
TCGATGCCATGCCCGAGCCGAAAAGGAAGGCGACCCGCACCAAGCAAGCGCTGGAGCTCTCAGCCTTCCAGGCAGTCAAG
CGCGACTTCGCTTTCGTGGTTGACCGGGACGTCGAAGCCGCCGCGATCGTCCGCGCCGCCACCGGCGCCGACCGGAAGCT
GGTGACCTCCGTCAACGTCTTCGACGTCTTCGAAGGCGCATCCCTCGGCGAGGGCAAGAAATCGGTGGCGATCGAGGTGA
CGATTCAGCCGGTCGAGCGCACGCTGACGGATGAGGACTTCGAGGCGTTGACCGCGCGCATCGTGGCCAATGTCGCGAAG
AGCACCGGCGGCGTACTGCGCGCTTGA

Upstream 100 bases:

>100_bases
CTGGATGACCCATTACGGCTTCCGCCCGCTCGACATGCCGACGCTCTTCGGCGGCCTCTCGGCGTAACCGGCGCGGAAAA
TCAAGGACACAGGTGACAAC

Downstream 100 bases:

>100_bases
TTAGCAAGGCCCTGGCCGCCTCGCGATGCGATGCCTCTCAGCCCTTTGCCCTTCTCTTCGGGGGGCAGAGGCGGCGGGGC
ACAGCCCAGCAGGCGGGGAG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 808; Mature: 808

Protein sequence:

>808_residues
MKFTLSWLKDHLETEASLEEICARLTMIGLEVEDVDDKAAFKPFVIAKVISAEQHPNADKLRVLMVDTGSGEPVQVVCGA
PNARKGLVGAFAAPGTYVPGIGVTLSVGNIRGVESRGMMCSEKELEISDDHNGIIDLPEDAPVGASYADFAGLDDPVIAI
NLTPNRPDCTSVHGIARDLAASGLGTLKTRPMPSFAVEGETPVKVRLDLGEDGHLCPGFALRLVRGVKNGPSPAWMRRRL
MAIGLRPINALVDITNYLTFDQGRPLHVFDAAKVAGNLTVRRAREGEKVLALDTREYMLSPANVVIADEEGVESIGGIMG
GEHSGCDETTTDVLIESALWDPMNIARTGRTLSIITDARYRFERGVDPEYMVPGLERATELVLELCGGTAARMDVVGYDG
HTPKRADFPVSEVKRLTGLEVSSEESVSILRKLGFGVEGSGERLTVKVPSWRPDIDGKADLVEEVMRIHGVDNIVAAPLA
SHNAVNGRILTVLQIRTRQARRALASRGMLEAVTWSFIPQEHAKLFGGGQPELKLANPIAADMSDMRPSLLPGLLAAAQR
NADKGHGDVAIFEVSGTYEGDTPGAQRRVAGGVRRGTASLNGAGRLWSNSMKGGGKPVDVFDAKADAIAVLEACGVPMAN
VQFEAGGPAWYHPGRSATIKLGPKIVLGAFGEFHPKTLDVLDVSGPLSGFEVYIDAMPEPKRKATRTKQALELSAFQAVK
RDFAFVVDRDVEAAAIVRAATGADRKLVTSVNVFDVFEGASLGEGKKSVAIEVTIQPVERTLTDEDFEALTARIVANVAK
STGGVLRA

Sequences:

>Translated_808_residues
MKFTLSWLKDHLETEASLEEICARLTMIGLEVEDVDDKAAFKPFVIAKVISAEQHPNADKLRVLMVDTGSGEPVQVVCGA
PNARKGLVGAFAAPGTYVPGIGVTLSVGNIRGVESRGMMCSEKELEISDDHNGIIDLPEDAPVGASYADFAGLDDPVIAI
NLTPNRPDCTSVHGIARDLAASGLGTLKTRPMPSFAVEGETPVKVRLDLGEDGHLCPGFALRLVRGVKNGPSPAWMRRRL
MAIGLRPINALVDITNYLTFDQGRPLHVFDAAKVAGNLTVRRAREGEKVLALDTREYMLSPANVVIADEEGVESIGGIMG
GEHSGCDETTTDVLIESALWDPMNIARTGRTLSIITDARYRFERGVDPEYMVPGLERATELVLELCGGTAARMDVVGYDG
HTPKRADFPVSEVKRLTGLEVSSEESVSILRKLGFGVEGSGERLTVKVPSWRPDIDGKADLVEEVMRIHGVDNIVAAPLA
SHNAVNGRILTVLQIRTRQARRALASRGMLEAVTWSFIPQEHAKLFGGGQPELKLANPIAADMSDMRPSLLPGLLAAAQR
NADKGHGDVAIFEVSGTYEGDTPGAQRRVAGGVRRGTASLNGAGRLWSNSMKGGGKPVDVFDAKADAIAVLEACGVPMAN
VQFEAGGPAWYHPGRSATIKLGPKIVLGAFGEFHPKTLDVLDVSGPLSGFEVYIDAMPEPKRKATRTKQALELSAFQAVK
RDFAFVVDRDVEAAAIVRAATGADRKLVTSVNVFDVFEGASLGEGKKSVAIEVTIQPVERTLTDEDFEALTARIVANVAK
STGGVLRA
>Mature_808_residues
MKFTLSWLKDHLETEASLEEICARLTMIGLEVEDVDDKAAFKPFVIAKVISAEQHPNADKLRVLMVDTGSGEPVQVVCGA
PNARKGLVGAFAAPGTYVPGIGVTLSVGNIRGVESRGMMCSEKELEISDDHNGIIDLPEDAPVGASYADFAGLDDPVIAI
NLTPNRPDCTSVHGIARDLAASGLGTLKTRPMPSFAVEGETPVKVRLDLGEDGHLCPGFALRLVRGVKNGPSPAWMRRRL
MAIGLRPINALVDITNYLTFDQGRPLHVFDAAKVAGNLTVRRAREGEKVLALDTREYMLSPANVVIADEEGVESIGGIMG
GEHSGCDETTTDVLIESALWDPMNIARTGRTLSIITDARYRFERGVDPEYMVPGLERATELVLELCGGTAARMDVVGYDG
HTPKRADFPVSEVKRLTGLEVSSEESVSILRKLGFGVEGSGERLTVKVPSWRPDIDGKADLVEEVMRIHGVDNIVAAPLA
SHNAVNGRILTVLQIRTRQARRALASRGMLEAVTWSFIPQEHAKLFGGGQPELKLANPIAADMSDMRPSLLPGLLAAAQR
NADKGHGDVAIFEVSGTYEGDTPGAQRRVAGGVRRGTASLNGAGRLWSNSMKGGGKPVDVFDAKADAIAVLEACGVPMAN
VQFEAGGPAWYHPGRSATIKLGPKIVLGAFGEFHPKTLDVLDVSGPLSGFEVYIDAMPEPKRKATRTKQALELSAFQAVK
RDFAFVVDRDVEAAAIVRAATGADRKLVTSVNVFDVFEGASLGEGKKSVAIEVTIQPVERTLTDEDFEALTARIVANVAK
STGGVLRA

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=817, Percent_Identity=36.4749082007344, Blast_Score=456, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21355211, Length=406, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86307; Mature: 86307

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKDHLETEASLEEICARLTMIGLEVEDVDDKAAFKPFVIAKVISAEQHPNADK
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHCCHHHHHHHHHHCCCCCCCC
LRVLMVDTGSGEPVQVVCGAPNARKGLVGAFAAPGTYVPGIGVTLSVGNIRGVESRGMMC
EEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEE
SEKELEISDDHNGIIDLPEDAPVGASYADFAGLDDPVIAINLTPNRPDCTSVHGIARDLA
CCCCCEECCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ASGLGTLKTRPMPSFAVEGETPVKVRLDLGEDGHLCPGFALRLVRGVKNGPSPAWMRRRL
HCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
MAIGLRPINALVDITNYLTFDQGRPLHVFDAAKVAGNLTVRRAREGEKVLALDTREYMLS
HHHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHHCCEEEEECCCCCEEEEEECHHHHCC
PANVVIADEEGVESIGGIMGGEHSGCDETTTDVLIESALWDPMNIARTGRTLSIITDARY
CCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEEECCHH
RFERGVDPEYMVPGLERATELVLELCGGTAARMDVVGYDGHTPKRADFPVSEVKRLTGLE
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCC
VSSEESVSILRKLGFGVEGSGERLTVKVPSWRPDIDGKADLVEEVMRIHGVDNIVAAPLA
CCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
SHNAVNGRILTVLQIRTRQARRALASRGMLEAVTWSFIPQEHAKLFGGGQPELKLANPIA
CCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEECCCCH
ADMSDMRPSLLPGLLAAAQRNADKGHGDVAIFEVSGTYEGDTPGAQRRVAGGVRRGTASL
HCHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCHHCCCCCC
NGAGRLWSNSMKGGGKPVDVFDAKADAIAVLEACGVPMANVQFEAGGPAWYHPGRSATIK
CCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCEECCCCCEEEE
LGPKIVLGAFGEFHPKTLDVLDVSGPLSGFEVYIDAMPEPKRKATRTKQALELSAFQAVK
ECCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RDFAFVVDRDVEAAAIVRAATGADRKLVTSVNVFDVFEGASLGEGKKSVAIEVTIQPVER
HHHEEEEECCCCHHHHEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEEEEEECHHH
TLTDEDFEALTARIVANVAKSTGGVLRA
HCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLSWLKDHLETEASLEEICARLTMIGLEVEDVDDKAAFKPFVIAKVISAEQHPNADK
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHCCHHHHHHHHHHCCCCCCCC
LRVLMVDTGSGEPVQVVCGAPNARKGLVGAFAAPGTYVPGIGVTLSVGNIRGVESRGMMC
EEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCEE
SEKELEISDDHNGIIDLPEDAPVGASYADFAGLDDPVIAINLTPNRPDCTSVHGIARDLA
CCCCCEECCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ASGLGTLKTRPMPSFAVEGETPVKVRLDLGEDGHLCPGFALRLVRGVKNGPSPAWMRRRL
HCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
MAIGLRPINALVDITNYLTFDQGRPLHVFDAAKVAGNLTVRRAREGEKVLALDTREYMLS
HHHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHHHCCEEEEECCCCCEEEEEECHHHHCC
PANVVIADEEGVESIGGIMGGEHSGCDETTTDVLIESALWDPMNIARTGRTLSIITDARY
CCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEEECCHH
RFERGVDPEYMVPGLERATELVLELCGGTAARMDVVGYDGHTPKRADFPVSEVKRLTGLE
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCC
VSSEESVSILRKLGFGVEGSGERLTVKVPSWRPDIDGKADLVEEVMRIHGVDNIVAAPLA
CCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
SHNAVNGRILTVLQIRTRQARRALASRGMLEAVTWSFIPQEHAKLFGGGQPELKLANPIA
CCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEECCCCH
ADMSDMRPSLLPGLLAAAQRNADKGHGDVAIFEVSGTYEGDTPGAQRRVAGGVRRGTASL
HCHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCHHCCCCCC
NGAGRLWSNSMKGGGKPVDVFDAKADAIAVLEACGVPMANVQFEAGGPAWYHPGRSATIK
CCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCEECCCCCEEEE
LGPKIVLGAFGEFHPKTLDVLDVSGPLSGFEVYIDAMPEPKRKATRTKQALELSAFQAVK
ECCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RDFAFVVDRDVEAAAIVRAATGADRKLVTSVNVFDVFEGASLGEGKKSVAIEVTIQPVER
HHHEEEEECCCCHHHHEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEEEEEECHHH
TLTDEDFEALTARIVANVAKSTGGVLRA
HCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]