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The map label for this gene is pheS

Identifier: 150398672

GI number: 150398672

Start: 3692417

End: 3693499

Strand: Direct

Name: pheS

Synonym: Smed_3486

Alternate gene names: 150398672

Gene position: 3692417-3693499 (Clockwise)

Preceding gene: 150398671

Following gene: 150398673

Centisome position: 97.63

GC content: 62.23

Gene sequence:

>1083_bases
ATGAGCGAACTGGAAACATTGGAACGTACCTTGCTGGCGGAGATCGAAGCCGCCGCCGACGAGGGAGCGATCGAGGCGGT
ACGCGTTGGCGCACTCGGCAAAAAGGGATCAATCTCGGAACTCCTGAAGACGCTCGGTTCGATGAGTGCGGAGGAGCGCC
AGACCCGCGGCGCCCGCATCAACGCGCTGAAGAACACGGTGAGCGAGGCGATCTCCGCCCGGAAGGCGGAGCTCAAGGAC
GCGGCGATCGCTGAGCGCCTGGCGCGCGAGACGGTGGACATCAGTCTTCCCGTCCGGTCCTCGCCGGCGGAGCGCGGCCG
CATCCATCCGATCAGCCAGATCGTCGATGAAATCACCGCGATTTTTGGCGATATGGGTTTCTCGCTCGCCGAGGGGCCGG
ATATCGAGACGGACTACTACAATTTCACGGCGCTGAATTTCCCGGAAGGCCACCCGGCCCGCGAGATGCACGACACCTTC
TTCTTCGCGCCGGACGAGAAGGGTGAGCGGAAGGTGCTGCGCACGCACACCTCTCCGGTGCAGATACGCACGATGGAAGC
CGAGCAGCCGCCGATCCGCATCATCATTCCCGGCAAGACCTATCGTCAGGATTCGGACGCGACCCACTCGCCGATGTTCC
ATCAGGTGGAAGGCCTCGTCATCGACAGGACGGCGAATGTCGCCAACATGCGGTGGGTACTCGAGGAATTCTGCAAGGCC
TTCTTCGAAGTGGATCAGGTGACGATGCGCTTCCGTCCGTCCTTCTTCCCCTTCACGGAACCCTCCTTCGAGGTGGATAT
CCAGTGCGACCGTTCCGGCCCGATCGTAAAGTTCGGCGAAGGCAAGGACTGGATGGAGATTCTCGGTTGCGGCATGGTGC
ATCCGAACGTGCTTCGGGCTGGCGGCCTCGACCCGGACGAATTTCAAGGCTTCGCCTGGGGCATGGGCCTCGATCGTATC
GCCATGCTGAAATACGGCATGCCGGACCTGCGCGACTTCTTCAATGCCGATGTCCGCTGGATGACCCATTACGGCTTCCG
CCCGCTCGACATGCCGACGCTCTTCGGCGGCCTCTCGGCGTAA

Upstream 100 bases:

>100_bases
GTTTTTTCTTGTTCGGCGCGCGGCGCGATCCTCTCGCTGACGTGTCGTGCCGATACACGGACCGCATCGAGGCAGGACAG
CCCGCATTGAGGCAGATGAG

Downstream 100 bases:

>100_bases
CCGGCGCGGAAAATCAAGGACACAGGTGACAACATGAAGTTCACGCTCTCCTGGTTGAAGGACCATCTGGAAACCGAAGC
CTCGCTCGAGGAAATCTGCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSELETLERTLLAEIEAAADEGAIEAVRVGALGKKGSISELLKTLGSMSAEERQTRGARINALKNTVSEAISARKAELKD
AAIAERLARETVDISLPVRSSPAERGRIHPISQIVDEITAIFGDMGFSLAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFAPDEKGERKVLRTHTSPVQIRTMEAEQPPIRIIIPGKTYRQDSDATHSPMFHQVEGLVIDRTANVANMRWVLEEFCKA
FFEVDQVTMRFRPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEFQGFAWGMGLDRI
AMLKYGMPDLRDFFNADVRWMTHYGFRPLDMPTLFGGLSA

Sequences:

>Translated_360_residues
MSELETLERTLLAEIEAAADEGAIEAVRVGALGKKGSISELLKTLGSMSAEERQTRGARINALKNTVSEAISARKAELKD
AAIAERLARETVDISLPVRSSPAERGRIHPISQIVDEITAIFGDMGFSLAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFAPDEKGERKVLRTHTSPVQIRTMEAEQPPIRIIIPGKTYRQDSDATHSPMFHQVEGLVIDRTANVANMRWVLEEFCKA
FFEVDQVTMRFRPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEFQGFAWGMGLDRI
AMLKYGMPDLRDFFNADVRWMTHYGFRPLDMPTLFGGLSA
>Mature_359_residues
SELETLERTLLAEIEAAADEGAIEAVRVGALGKKGSISELLKTLGSMSAEERQTRGARINALKNTVSEAISARKAELKDA
AIAERLARETVDISLPVRSSPAERGRIHPISQIVDEITAIFGDMGFSLAEGPDIETDYYNFTALNFPEGHPAREMHDTFF
FAPDEKGERKVLRTHTSPVQIRTMEAEQPPIRIIIPGKTYRQDSDATHSPMFHQVEGLVIDRTANVANMRWVLEEFCKAF
FEVDQVTMRFRPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEFQGFAWGMGLDRIA
MLKYGMPDLRDFFNADVRWMTHYGFRPLDMPTLFGGLSA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=283, Percent_Identity=30.0353356890459, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI5729820, Length=293, Percent_Identity=26.6211604095563, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=50.2906976744186, Blast_Score=351, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=31.021897810219, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=31.021897810219, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=281, Percent_Identity=29.1814946619217, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=31.2727272727273, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=36.1702127659575, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=280, Percent_Identity=31.0714285714286, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=299, Percent_Identity=23.7458193979933, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_SINMW (A6UF74)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001329139.1
- ProteinModelPortal:   A6UF74
- SMR:   A6UF74
- STRING:   A6UF74
- GeneID:   5324374
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3486
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   SMED366394:SMED_3486-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40283; Mature: 40152

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELETLERTLLAEIEAAADEGAIEAVRVGALGKKGSISELLKTLGSMSAEERQTRGARI
CCHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCHHHHHHHHHCCCHHHHHHCCCHH
NALKNTVSEAISARKAELKDAAIAERLARETVDISLPVRSSPAERGRIHPISQIVDEITA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IFGDMGFSLAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFAPDEKGERKVLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCEEECCCCCCCHHHHHCCCCCE
QIRTMEAEQPPIRIIIPGKTYRQDSDATHSPMFHQVEGLVIDRTANVANMRWVLEEFCKA
EEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFEVDQVTMRFRPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRA
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCHHHC
GGLDPDEFQGFAWGMGLDRIAMLKYGMPDLRDFFNADVRWMTHYGFRPLDMPTLFGGLSA
CCCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SELETLERTLLAEIEAAADEGAIEAVRVGALGKKGSISELLKTLGSMSAEERQTRGARI
CHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCHHHHHHHHHCCCHHHHHHCCCHH
NALKNTVSEAISARKAELKDAAIAERLARETVDISLPVRSSPAERGRIHPISQIVDEITA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IFGDMGFSLAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFAPDEKGERKVLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCEEECCCCCCCHHHHHCCCCCE
QIRTMEAEQPPIRIIIPGKTYRQDSDATHSPMFHQVEGLVIDRTANVANMRWVLEEFCKA
EEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFEVDQVTMRFRPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRA
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCHHHC
GGLDPDEFQGFAWGMGLDRIAMLKYGMPDLRDFFNADVRWMTHYGFRPLDMPTLFGGLSA
CCCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA